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Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatogra...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562736/ https://www.ncbi.nlm.nih.gov/pubmed/26366080 http://dx.doi.org/10.2147/IJN.S82166 |
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author | Zhou, Xiaoyue Park, Shin-Hye Mao, Hongli Isoshima, Takashi Wang, Yi Ito, Yoshihiro |
author_facet | Zhou, Xiaoyue Park, Shin-Hye Mao, Hongli Isoshima, Takashi Wang, Yi Ito, Yoshihiro |
author_sort | Zhou, Xiaoyue |
collection | PubMed |
description | Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface. |
format | Online Article Text |
id | pubmed-4562736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45627362015-09-11 Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement Zhou, Xiaoyue Park, Shin-Hye Mao, Hongli Isoshima, Takashi Wang, Yi Ito, Yoshihiro Int J Nanomedicine Original Research Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface. Dove Medical Press 2015-09-02 /pmc/articles/PMC4562736/ /pubmed/26366080 http://dx.doi.org/10.2147/IJN.S82166 Text en © 2015 Zhou et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Zhou, Xiaoyue Park, Shin-Hye Mao, Hongli Isoshima, Takashi Wang, Yi Ito, Yoshihiro Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement |
title | Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement |
title_full | Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement |
title_fullStr | Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement |
title_full_unstemmed | Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement |
title_short | Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement |
title_sort | nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562736/ https://www.ncbi.nlm.nih.gov/pubmed/26366080 http://dx.doi.org/10.2147/IJN.S82166 |
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