Photo-activatable Cre recombinase regulates gene expression in vivo

Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modify gene expression in the mouse brain. Blue light illumination for 12 hours via optical fibe...

Descripción completa

Detalles Bibliográficos
Autores principales: Schindler, Suzanne E., McCall, Jordan G., Yan, Ping, Hyrc, Krzystof L., Li, Mingjie, Tucker, Chandra L., Lee, Jin-Moo, Bruchas, Michael R., Diamond, Marc I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4563371/
https://www.ncbi.nlm.nih.gov/pubmed/26350769
http://dx.doi.org/10.1038/srep13627
Descripción
Sumario:Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modify gene expression in the mouse brain. Blue light illumination for 12 hours via optical fibers activated PA-Cre in the hippocampus, a deep brain structure. Two-photon illumination through a thinned skull window for 100 minutes activated PA-Cre within a sub-millimeter region of cortex. Light activation of PA-Cre may allow permanent gene modification with improved spatiotemporal precision compared to standard methods.

Ejemplares similares