Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry

Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60–80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Charretier, Yannick, Dauwalder, Olivier, Franceschi, Christine, Degout-Charmette, Elodie, Zambardi, Gilles, Cecchini, Tiphaine, Bardet, Chloe, Lacoux, Xavier, Dufour, Philippe, Veron, Laurent, Rostaing, Hervé, Lanet, Veronique, Fortin, Tanguy, Beaulieu, Corinne, Perrot, Nadine, Dechaume, Dominique, Pons, Sylvie, Girard, Victoria, Salvador, Arnaud, Durand, Géraldine, Mallard, Frédéric, Theretz, Alain, Broyer, Patrick, Chatellier, Sonia, Gervasi, Gaspard, Van Nuenen, Marc, Ann Roitsch, Carolyn, Van Belkum, Alex, Lemoine, Jérôme, Vandenesch, François, Charrier, Jean-Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4563557/
https://www.ncbi.nlm.nih.gov/pubmed/26350205
http://dx.doi.org/10.1038/srep13944
Descripción
Sumario:Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60–80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients.

Ejemplares similares