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Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry
Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60–80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it pr...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4563557/ https://www.ncbi.nlm.nih.gov/pubmed/26350205 http://dx.doi.org/10.1038/srep13944 |
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| author | Charretier, Yannick Dauwalder, Olivier Franceschi, Christine Degout-Charmette, Elodie Zambardi, Gilles Cecchini, Tiphaine Bardet, Chloe Lacoux, Xavier Dufour, Philippe Veron, Laurent Rostaing, Hervé Lanet, Veronique Fortin, Tanguy Beaulieu, Corinne Perrot, Nadine Dechaume, Dominique Pons, Sylvie Girard, Victoria Salvador, Arnaud Durand, Géraldine Mallard, Frédéric Theretz, Alain Broyer, Patrick Chatellier, Sonia Gervasi, Gaspard Van Nuenen, Marc Ann Roitsch, Carolyn Van Belkum, Alex Lemoine, Jérôme Vandenesch, François Charrier, Jean-Philippe |
| author_facet | Charretier, Yannick Dauwalder, Olivier Franceschi, Christine Degout-Charmette, Elodie Zambardi, Gilles Cecchini, Tiphaine Bardet, Chloe Lacoux, Xavier Dufour, Philippe Veron, Laurent Rostaing, Hervé Lanet, Veronique Fortin, Tanguy Beaulieu, Corinne Perrot, Nadine Dechaume, Dominique Pons, Sylvie Girard, Victoria Salvador, Arnaud Durand, Géraldine Mallard, Frédéric Theretz, Alain Broyer, Patrick Chatellier, Sonia Gervasi, Gaspard Van Nuenen, Marc Ann Roitsch, Carolyn Van Belkum, Alex Lemoine, Jérôme Vandenesch, François Charrier, Jean-Philippe |
| author_sort | Charretier, Yannick |
| collection | PubMed |
| description | Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60–80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients. |
| format | Online Article Text |
| id | pubmed-4563557 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-45635572015-09-15 Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry Charretier, Yannick Dauwalder, Olivier Franceschi, Christine Degout-Charmette, Elodie Zambardi, Gilles Cecchini, Tiphaine Bardet, Chloe Lacoux, Xavier Dufour, Philippe Veron, Laurent Rostaing, Hervé Lanet, Veronique Fortin, Tanguy Beaulieu, Corinne Perrot, Nadine Dechaume, Dominique Pons, Sylvie Girard, Victoria Salvador, Arnaud Durand, Géraldine Mallard, Frédéric Theretz, Alain Broyer, Patrick Chatellier, Sonia Gervasi, Gaspard Van Nuenen, Marc Ann Roitsch, Carolyn Van Belkum, Alex Lemoine, Jérôme Vandenesch, François Charrier, Jean-Philippe Sci Rep Article Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60–80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients. Nature Publishing Group 2015-09-09 /pmc/articles/PMC4563557/ /pubmed/26350205 http://dx.doi.org/10.1038/srep13944 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Article Charretier, Yannick Dauwalder, Olivier Franceschi, Christine Degout-Charmette, Elodie Zambardi, Gilles Cecchini, Tiphaine Bardet, Chloe Lacoux, Xavier Dufour, Philippe Veron, Laurent Rostaing, Hervé Lanet, Veronique Fortin, Tanguy Beaulieu, Corinne Perrot, Nadine Dechaume, Dominique Pons, Sylvie Girard, Victoria Salvador, Arnaud Durand, Géraldine Mallard, Frédéric Theretz, Alain Broyer, Patrick Chatellier, Sonia Gervasi, Gaspard Van Nuenen, Marc Ann Roitsch, Carolyn Van Belkum, Alex Lemoine, Jérôme Vandenesch, François Charrier, Jean-Philippe Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry |
| title | Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry |
| title_full | Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry |
| title_fullStr | Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry |
| title_full_unstemmed | Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry |
| title_short | Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry |
| title_sort | rapid bacterial identification, resistance, virulence and type profiling using selected reaction monitoring mass spectrometry |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4563557/ https://www.ncbi.nlm.nih.gov/pubmed/26350205 http://dx.doi.org/10.1038/srep13944 |
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