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Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences

In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of singl...

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Autores principales: Back, Chang-Gi, Lee, Seung-Yeol, Lee, Boo-Ja, Yea, Mi-Chi, Kim, Sang-Mok, Kang, In-Kyu, Cha, Jae-Soon, Jung, Hee-Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564146/
https://www.ncbi.nlm.nih.gov/pubmed/26361469
http://dx.doi.org/10.5423/PPJ.OA.04.2015.0049
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author Back, Chang-Gi
Lee, Seung-Yeol
Lee, Boo-Ja
Yea, Mi-Chi
Kim, Sang-Mok
Kang, In-Kyu
Cha, Jae-Soon
Jung, Hee-Young
author_facet Back, Chang-Gi
Lee, Seung-Yeol
Lee, Boo-Ja
Yea, Mi-Chi
Kim, Sang-Mok
Kang, In-Kyu
Cha, Jae-Soon
Jung, Hee-Young
author_sort Back, Chang-Gi
collection PubMed
description In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the host DNA and the microbial community of the host surface. In addition, a detection limit of 1 pg/μl per PCR reaction was detected when these primer sets were used to amplify corresponding bacterial DNAs. Therefore, these primer sets and the developed species-specific PCR assay represent a valuable, sensitive, and rapid diagnostic tool that can be used to detect three specific pathogens at early stages of infection and may help control diseases.
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spelling pubmed-45641462015-09-10 Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences Back, Chang-Gi Lee, Seung-Yeol Lee, Boo-Ja Yea, Mi-Chi Kim, Sang-Mok Kang, In-Kyu Cha, Jae-Soon Jung, Hee-Young Plant Pathol J Articles In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the host DNA and the microbial community of the host surface. In addition, a detection limit of 1 pg/μl per PCR reaction was detected when these primer sets were used to amplify corresponding bacterial DNAs. Therefore, these primer sets and the developed species-specific PCR assay represent a valuable, sensitive, and rapid diagnostic tool that can be used to detect three specific pathogens at early stages of infection and may help control diseases. Korean Society of Plant Pathology 2015-09 2015-09-30 /pmc/articles/PMC4564146/ /pubmed/26361469 http://dx.doi.org/10.5423/PPJ.OA.04.2015.0049 Text en © The Korean Society of Plant Pathology
spellingShingle Articles
Back, Chang-Gi
Lee, Seung-Yeol
Lee, Boo-Ja
Yea, Mi-Chi
Kim, Sang-Mok
Kang, In-Kyu
Cha, Jae-Soon
Jung, Hee-Young
Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
title Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
title_full Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
title_fullStr Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
title_full_unstemmed Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
title_short Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
title_sort development of a species-specific pcr assay for three xanthomonas species, causing bulb and flower diseases, based on their genome sequences
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564146/
https://www.ncbi.nlm.nih.gov/pubmed/26361469
http://dx.doi.org/10.5423/PPJ.OA.04.2015.0049
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