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Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy

To improve the androst-1,4-diene-3,17-dione (ADD) production from phytosterol by Mycobacterium neoaurum JC-12, fructose was firstly found favorable as the initial carbon source to increase the biomass and eliminate the lag phase of M. neoaurum JC-12 in the phytosterol transformation process. Based o...

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Detalles Bibliográficos
Autores principales: Shao, Minglong, Zhang, Xian, Rao, Zhiming, Xu, Meijuan, Yang, Taowei, Li, Hui, Xu, Zhenghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564235/
https://www.ncbi.nlm.nih.gov/pubmed/26352898
http://dx.doi.org/10.1371/journal.pone.0137658
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author Shao, Minglong
Zhang, Xian
Rao, Zhiming
Xu, Meijuan
Yang, Taowei
Li, Hui
Xu, Zhenghong
author_facet Shao, Minglong
Zhang, Xian
Rao, Zhiming
Xu, Meijuan
Yang, Taowei
Li, Hui
Xu, Zhenghong
author_sort Shao, Minglong
collection PubMed
description To improve the androst-1,4-diene-3,17-dione (ADD) production from phytosterol by Mycobacterium neoaurum JC-12, fructose was firstly found favorable as the initial carbon source to increase the biomass and eliminate the lag phase of M. neoaurum JC-12 in the phytosterol transformation process. Based on this phenomenon, two-stage fermentation by using fructose as the initial carbon source and feeding glucose to maintain strain metabolism was designed. By applying this strategy, the fermentation duration was decreased from 168 h to 120 h with the ADD productivity increased from 0.071 g/(L·h) to 0.108 g/(L·h). Further, three-stage fermentation by adding phytosterol to improve ADD production at the end of the two-stage fermentation was carried out and the final ADD production reached 18.6 g/L, which is the highest reported ADD production using phytosterol as substrate. Thus, this strategy provides a possible way in enhancing the ADD production in pharmaceutical industry.
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spelling pubmed-45642352015-09-17 Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy Shao, Minglong Zhang, Xian Rao, Zhiming Xu, Meijuan Yang, Taowei Li, Hui Xu, Zhenghong PLoS One Research Article To improve the androst-1,4-diene-3,17-dione (ADD) production from phytosterol by Mycobacterium neoaurum JC-12, fructose was firstly found favorable as the initial carbon source to increase the biomass and eliminate the lag phase of M. neoaurum JC-12 in the phytosterol transformation process. Based on this phenomenon, two-stage fermentation by using fructose as the initial carbon source and feeding glucose to maintain strain metabolism was designed. By applying this strategy, the fermentation duration was decreased from 168 h to 120 h with the ADD productivity increased from 0.071 g/(L·h) to 0.108 g/(L·h). Further, three-stage fermentation by adding phytosterol to improve ADD production at the end of the two-stage fermentation was carried out and the final ADD production reached 18.6 g/L, which is the highest reported ADD production using phytosterol as substrate. Thus, this strategy provides a possible way in enhancing the ADD production in pharmaceutical industry. Public Library of Science 2015-09-09 /pmc/articles/PMC4564235/ /pubmed/26352898 http://dx.doi.org/10.1371/journal.pone.0137658 Text en © 2015 Shao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shao, Minglong
Zhang, Xian
Rao, Zhiming
Xu, Meijuan
Yang, Taowei
Li, Hui
Xu, Zhenghong
Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy
title Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy
title_full Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy
title_fullStr Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy
title_full_unstemmed Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy
title_short Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy
title_sort enhanced production of androst-1,4-diene-3,17-dione by mycobacterium neoaurum jc-12 using three-stage fermentation strategy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564235/
https://www.ncbi.nlm.nih.gov/pubmed/26352898
http://dx.doi.org/10.1371/journal.pone.0137658
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