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Detection of circulating IgG antibodies to apolipoprotein B(100) in acute myocardial infarction
A number of studies have reported an association between increased levels of antibodies against oxidized low-density lipoprotein (oxLDL) and cardiovascular disease, but the anti-oxLDL antibody has not been confirmed to serve as an effective biomarker for prediction of acute myocardial infarction (AM...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564368/ https://www.ncbi.nlm.nih.gov/pubmed/26425439 http://dx.doi.org/10.1016/j.fob.2015.08.006 |
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author | Zhang, Xueying Zhang, Xiaohong Lei, Mingming Lin, Yingzi Megson, Ian L. Wei, Jun Yu, Bo Jin, Yuanzhe |
author_facet | Zhang, Xueying Zhang, Xiaohong Lei, Mingming Lin, Yingzi Megson, Ian L. Wei, Jun Yu, Bo Jin, Yuanzhe |
author_sort | Zhang, Xueying |
collection | PubMed |
description | A number of studies have reported an association between increased levels of antibodies against oxidized low-density lipoprotein (oxLDL) and cardiovascular disease, but the anti-oxLDL antibody has not been confirmed to serve as an effective biomarker for prediction of acute myocardial infarction (AMI). Apolipoprotein B(100) (ApoB(100))-derived peptide fragments generated by proteolytic degradation and aldehyde modification are the major antigens in oxLDL, and so the present work was undertaken to detect circulating IgG for Apo-B(100)-derived peptide antigens. An in-house enzyme-linked immunosorbent assay (ELISA) was developed with eight ApoB(100)-derived peptide antigens (Ag1–Ag8) to detect circulating anti-ApoB(100) IgG levels in 267 patients with AMI and 201 control subjects. Binary logistic regression analysis revealed that circulating IgG for Ag1 was significantly higher in the patient group than the control group (P < 0.001) after adjustment for age, gender, smoking, hypertension, diabetes and circulating levels of cholesterol, HDL, LDL, ApoA and ApoB(100). None of the other seven antigens detected an increase in IgG levels in AMI patients compared with control subjects. Spearman correlation analysis showed no correlation between IgG antibody for Ag1 and clinical characteristics. In conclusion, the linear peptide antigens derived from ApoB(100) may be suitable for the development of an ELISA antibody test for prediction of AMI, although further confirmation is still needed in large-scale clinical studies. |
format | Online Article Text |
id | pubmed-4564368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-45643682015-09-30 Detection of circulating IgG antibodies to apolipoprotein B(100) in acute myocardial infarction Zhang, Xueying Zhang, Xiaohong Lei, Mingming Lin, Yingzi Megson, Ian L. Wei, Jun Yu, Bo Jin, Yuanzhe FEBS Open Bio Research article A number of studies have reported an association between increased levels of antibodies against oxidized low-density lipoprotein (oxLDL) and cardiovascular disease, but the anti-oxLDL antibody has not been confirmed to serve as an effective biomarker for prediction of acute myocardial infarction (AMI). Apolipoprotein B(100) (ApoB(100))-derived peptide fragments generated by proteolytic degradation and aldehyde modification are the major antigens in oxLDL, and so the present work was undertaken to detect circulating IgG for Apo-B(100)-derived peptide antigens. An in-house enzyme-linked immunosorbent assay (ELISA) was developed with eight ApoB(100)-derived peptide antigens (Ag1–Ag8) to detect circulating anti-ApoB(100) IgG levels in 267 patients with AMI and 201 control subjects. Binary logistic regression analysis revealed that circulating IgG for Ag1 was significantly higher in the patient group than the control group (P < 0.001) after adjustment for age, gender, smoking, hypertension, diabetes and circulating levels of cholesterol, HDL, LDL, ApoA and ApoB(100). None of the other seven antigens detected an increase in IgG levels in AMI patients compared with control subjects. Spearman correlation analysis showed no correlation between IgG antibody for Ag1 and clinical characteristics. In conclusion, the linear peptide antigens derived from ApoB(100) may be suitable for the development of an ELISA antibody test for prediction of AMI, although further confirmation is still needed in large-scale clinical studies. Elsevier 2015-08-11 /pmc/articles/PMC4564368/ /pubmed/26425439 http://dx.doi.org/10.1016/j.fob.2015.08.006 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research article Zhang, Xueying Zhang, Xiaohong Lei, Mingming Lin, Yingzi Megson, Ian L. Wei, Jun Yu, Bo Jin, Yuanzhe Detection of circulating IgG antibodies to apolipoprotein B(100) in acute myocardial infarction |
title | Detection of circulating IgG antibodies to apolipoprotein B(100) in acute myocardial infarction |
title_full | Detection of circulating IgG antibodies to apolipoprotein B(100) in acute myocardial infarction |
title_fullStr | Detection of circulating IgG antibodies to apolipoprotein B(100) in acute myocardial infarction |
title_full_unstemmed | Detection of circulating IgG antibodies to apolipoprotein B(100) in acute myocardial infarction |
title_short | Detection of circulating IgG antibodies to apolipoprotein B(100) in acute myocardial infarction |
title_sort | detection of circulating igg antibodies to apolipoprotein b(100) in acute myocardial infarction |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564368/ https://www.ncbi.nlm.nih.gov/pubmed/26425439 http://dx.doi.org/10.1016/j.fob.2015.08.006 |
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