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Ischemic Postconditioning and Subanesthetic S(+)-Ketamine Infusion: Effects on Renal Function and Histology in Rats

Background. Ischemic postconditioning (IP) in renal Ischemia reperfusion injury (IRI) models improves renal function after IRI. Ketamine affords significant benefits against IRI-induced acute kidney injury (AKI). The present study investigated the effects of IP and IP associated with subanesthetic S...

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Autores principales: de Resende, Marco A. C., Pantoja, Alberto V., Barcellos, Bruno M., Reis, Eduardo P., Consolo, Thays D., Módolo, Renata P., Domingues, Maria A. C., Assad, Alexandra R., Cavalcanti, Ismar L., Castiglia, Yara M. M., Módolo, Norma S. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564631/
https://www.ncbi.nlm.nih.gov/pubmed/26413552
http://dx.doi.org/10.1155/2015/864902
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author de Resende, Marco A. C.
Pantoja, Alberto V.
Barcellos, Bruno M.
Reis, Eduardo P.
Consolo, Thays D.
Módolo, Renata P.
Domingues, Maria A. C.
Assad, Alexandra R.
Cavalcanti, Ismar L.
Castiglia, Yara M. M.
Módolo, Norma S. P.
author_facet de Resende, Marco A. C.
Pantoja, Alberto V.
Barcellos, Bruno M.
Reis, Eduardo P.
Consolo, Thays D.
Módolo, Renata P.
Domingues, Maria A. C.
Assad, Alexandra R.
Cavalcanti, Ismar L.
Castiglia, Yara M. M.
Módolo, Norma S. P.
author_sort de Resende, Marco A. C.
collection PubMed
description Background. Ischemic postconditioning (IP) in renal Ischemia reperfusion injury (IRI) models improves renal function after IRI. Ketamine affords significant benefits against IRI-induced acute kidney injury (AKI). The present study investigated the effects of IP and IP associated with subanesthetic S(+)-ketamine in ischemia-reperfusion-induced AKI. Methods. Forty-one Wistar rats were randomized into four groups: CG (10), control; KG (10), S(+)-ketamine infusion; IPG (10), IP; and KIPG (11), S(+)-ketamine infusion + IP. All rats underwent right nephrectomy. IRI and IP were induced only in IPG and KIPG by left kidney arterial occlusion for 30 min followed by reperfusion for 24 h. Complete reperfusion was preceded by three cycles of 2 min of reocclusion followed by 2 min of reperfusion. Renal function was assessed by measuring serum neutrophil gelatinase-associated lipocalin (NGAL), creatinine, and blood urea nitrogen (BUN). Tubular damage was evaluated by renal histology. Results. Creatinine and BUN were significantly increased. Severe tubular injury was only observed in the groups with IRI (IPG and KIPG), whereas no injury was observed in CG or KG. No significant differences were detected between IPG and KIPG. Conclusions. No synergic effect of the use of subanesthetic S(+)-ketamine and IP on AKI was observed in this rat model.
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spelling pubmed-45646312015-09-27 Ischemic Postconditioning and Subanesthetic S(+)-Ketamine Infusion: Effects on Renal Function and Histology in Rats de Resende, Marco A. C. Pantoja, Alberto V. Barcellos, Bruno M. Reis, Eduardo P. Consolo, Thays D. Módolo, Renata P. Domingues, Maria A. C. Assad, Alexandra R. Cavalcanti, Ismar L. Castiglia, Yara M. M. Módolo, Norma S. P. Biomed Res Int Research Article Background. Ischemic postconditioning (IP) in renal Ischemia reperfusion injury (IRI) models improves renal function after IRI. Ketamine affords significant benefits against IRI-induced acute kidney injury (AKI). The present study investigated the effects of IP and IP associated with subanesthetic S(+)-ketamine in ischemia-reperfusion-induced AKI. Methods. Forty-one Wistar rats were randomized into four groups: CG (10), control; KG (10), S(+)-ketamine infusion; IPG (10), IP; and KIPG (11), S(+)-ketamine infusion + IP. All rats underwent right nephrectomy. IRI and IP were induced only in IPG and KIPG by left kidney arterial occlusion for 30 min followed by reperfusion for 24 h. Complete reperfusion was preceded by three cycles of 2 min of reocclusion followed by 2 min of reperfusion. Renal function was assessed by measuring serum neutrophil gelatinase-associated lipocalin (NGAL), creatinine, and blood urea nitrogen (BUN). Tubular damage was evaluated by renal histology. Results. Creatinine and BUN were significantly increased. Severe tubular injury was only observed in the groups with IRI (IPG and KIPG), whereas no injury was observed in CG or KG. No significant differences were detected between IPG and KIPG. Conclusions. No synergic effect of the use of subanesthetic S(+)-ketamine and IP on AKI was observed in this rat model. Hindawi Publishing Corporation 2015 2015-08-27 /pmc/articles/PMC4564631/ /pubmed/26413552 http://dx.doi.org/10.1155/2015/864902 Text en Copyright © 2015 Marco A. C. de Resende et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
de Resende, Marco A. C.
Pantoja, Alberto V.
Barcellos, Bruno M.
Reis, Eduardo P.
Consolo, Thays D.
Módolo, Renata P.
Domingues, Maria A. C.
Assad, Alexandra R.
Cavalcanti, Ismar L.
Castiglia, Yara M. M.
Módolo, Norma S. P.
Ischemic Postconditioning and Subanesthetic S(+)-Ketamine Infusion: Effects on Renal Function and Histology in Rats
title Ischemic Postconditioning and Subanesthetic S(+)-Ketamine Infusion: Effects on Renal Function and Histology in Rats
title_full Ischemic Postconditioning and Subanesthetic S(+)-Ketamine Infusion: Effects on Renal Function and Histology in Rats
title_fullStr Ischemic Postconditioning and Subanesthetic S(+)-Ketamine Infusion: Effects on Renal Function and Histology in Rats
title_full_unstemmed Ischemic Postconditioning and Subanesthetic S(+)-Ketamine Infusion: Effects on Renal Function and Histology in Rats
title_short Ischemic Postconditioning and Subanesthetic S(+)-Ketamine Infusion: Effects on Renal Function and Histology in Rats
title_sort ischemic postconditioning and subanesthetic s(+)-ketamine infusion: effects on renal function and histology in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564631/
https://www.ncbi.nlm.nih.gov/pubmed/26413552
http://dx.doi.org/10.1155/2015/864902
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