Cargando…

T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects

OBJECTIVE: The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects. METHODS: ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Liang-fu, Chen, Ou, Chu, Ting-gang, Ding, Jian, Yu, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Neurosurgical Society 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564740/
https://www.ncbi.nlm.nih.gov/pubmed/26361524
http://dx.doi.org/10.3340/jkns.2015.58.2.101
_version_ 1782389489935581184
author Jiang, Liang-fu
Chen, Ou
Chu, Ting-gang
Ding, Jian
Yu, Qing
author_facet Jiang, Liang-fu
Chen, Ou
Chu, Ting-gang
Ding, Jian
Yu, Qing
author_sort Jiang, Liang-fu
collection PubMed
description OBJECTIVE: The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects. METHODS: ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the following methods : Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft. RESULTS: At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of CD3(+), CD4(+), and CD8(+) subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of CD3(+), CD4(+), and CD8(+) subsets and the serum concentration of IL-2, TNF-α, and IFN-γ in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of CD3(+), CD4(+), and CD8(+) subsets and the serum concentration of IL-2, TNF-α, and IFN-γ were significantly reduced in group C (p<0.05). CONCLUSION: The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects.
format Online
Article
Text
id pubmed-4564740
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Korean Neurosurgical Society
record_format MEDLINE/PubMed
spelling pubmed-45647402015-09-10 T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects Jiang, Liang-fu Chen, Ou Chu, Ting-gang Ding, Jian Yu, Qing J Korean Neurosurg Soc Laboratory Investigation OBJECTIVE: The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects. METHODS: ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the following methods : Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft. RESULTS: At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of CD3(+), CD4(+), and CD8(+) subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of CD3(+), CD4(+), and CD8(+) subsets and the serum concentration of IL-2, TNF-α, and IFN-γ in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of CD3(+), CD4(+), and CD8(+) subsets and the serum concentration of IL-2, TNF-α, and IFN-γ were significantly reduced in group C (p<0.05). CONCLUSION: The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects. The Korean Neurosurgical Society 2015-08 2015-08-28 /pmc/articles/PMC4564740/ /pubmed/26361524 http://dx.doi.org/10.3340/jkns.2015.58.2.101 Text en Copyright © 2015 The Korean Neurosurgical Society http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Laboratory Investigation
Jiang, Liang-fu
Chen, Ou
Chu, Ting-gang
Ding, Jian
Yu, Qing
T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects
title T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects
title_full T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects
title_fullStr T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects
title_full_unstemmed T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects
title_short T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects
title_sort t lymphocyte subsets and cytokines in rats transplanted with adipose-derived mesenchymal stem cells and acellular nerve for repairing the nerve defects
topic Laboratory Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564740/
https://www.ncbi.nlm.nih.gov/pubmed/26361524
http://dx.doi.org/10.3340/jkns.2015.58.2.101
work_keys_str_mv AT jiangliangfu tlymphocytesubsetsandcytokinesinratstransplantedwithadiposederivedmesenchymalstemcellsandacellularnerveforrepairingthenervedefects
AT chenou tlymphocytesubsetsandcytokinesinratstransplantedwithadiposederivedmesenchymalstemcellsandacellularnerveforrepairingthenervedefects
AT chutinggang tlymphocytesubsetsandcytokinesinratstransplantedwithadiposederivedmesenchymalstemcellsandacellularnerveforrepairingthenervedefects
AT dingjian tlymphocytesubsetsandcytokinesinratstransplantedwithadiposederivedmesenchymalstemcellsandacellularnerveforrepairingthenervedefects
AT yuqing tlymphocytesubsetsandcytokinesinratstransplantedwithadiposederivedmesenchymalstemcellsandacellularnerveforrepairingthenervedefects