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Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay

BACKGROUND: In studies of perfluoroalkyl acids, the validity and comparability of measured concentrations may be affected by differences in the handling of biospecimens. We aimed to investigate whether measured plasma levels of perfluoroalkyl acids differed between blood samples subjected to delay a...

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Autores principales: Bach, Cathrine Carlsen, Henriksen, Tine Brink, Bossi, Rossana, Bech, Bodil Hammer, Fuglsang, Jens, Olsen, Jørn, Nohr, Ellen Aagaard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565678/
https://www.ncbi.nlm.nih.gov/pubmed/26356420
http://dx.doi.org/10.1371/journal.pone.0137768
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author Bach, Cathrine Carlsen
Henriksen, Tine Brink
Bossi, Rossana
Bech, Bodil Hammer
Fuglsang, Jens
Olsen, Jørn
Nohr, Ellen Aagaard
author_facet Bach, Cathrine Carlsen
Henriksen, Tine Brink
Bossi, Rossana
Bech, Bodil Hammer
Fuglsang, Jens
Olsen, Jørn
Nohr, Ellen Aagaard
author_sort Bach, Cathrine Carlsen
collection PubMed
description BACKGROUND: In studies of perfluoroalkyl acids, the validity and comparability of measured concentrations may be affected by differences in the handling of biospecimens. We aimed to investigate whether measured plasma levels of perfluoroalkyl acids differed between blood samples subjected to delay and transportation prior to processing and samples with immediate processing and freezing. METHODS: Pregnant women recruited at Aarhus University Hospital, Denmark, (n = 88) provided paired blood samples. For each pair of samples, one was immediately processed and plasma was frozen, and the other was delayed and transported as whole blood before processing and freezing of plasma (similar to the Danish National Birth Cohort). We measured 12 perfluoroalkyl acids and present results for compounds with more than 50% of samples above the lower limit of quantification. RESULTS: For samples taken in the winter, relative differences between the paired samples ranged between -77 and +38% for individual perfluoroalkyl acids. In most cases concentrations were lower in the delayed and transported samples, e.g. the relative difference was -29% (95% confidence interval -30; -27) for perfluorooctane sulfonate. For perfluorooctanoate there was no difference between the two setups [corresponding estimate 1% (0, 3)]. Differences were negligible in the summer for all compounds. CONCLUSIONS: Transport of blood samples and processing delay, similar to conditions applied in some large, population-based studies, may affect measured perfluoroalkyl acid concentrations, mainly when outdoor temperatures are low. Attention to processing conditions is needed in studies of perfluoroalkyl acid exposure in humans.
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spelling pubmed-45656782015-09-18 Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay Bach, Cathrine Carlsen Henriksen, Tine Brink Bossi, Rossana Bech, Bodil Hammer Fuglsang, Jens Olsen, Jørn Nohr, Ellen Aagaard PLoS One Research Article BACKGROUND: In studies of perfluoroalkyl acids, the validity and comparability of measured concentrations may be affected by differences in the handling of biospecimens. We aimed to investigate whether measured plasma levels of perfluoroalkyl acids differed between blood samples subjected to delay and transportation prior to processing and samples with immediate processing and freezing. METHODS: Pregnant women recruited at Aarhus University Hospital, Denmark, (n = 88) provided paired blood samples. For each pair of samples, one was immediately processed and plasma was frozen, and the other was delayed and transported as whole blood before processing and freezing of plasma (similar to the Danish National Birth Cohort). We measured 12 perfluoroalkyl acids and present results for compounds with more than 50% of samples above the lower limit of quantification. RESULTS: For samples taken in the winter, relative differences between the paired samples ranged between -77 and +38% for individual perfluoroalkyl acids. In most cases concentrations were lower in the delayed and transported samples, e.g. the relative difference was -29% (95% confidence interval -30; -27) for perfluorooctane sulfonate. For perfluorooctanoate there was no difference between the two setups [corresponding estimate 1% (0, 3)]. Differences were negligible in the summer for all compounds. CONCLUSIONS: Transport of blood samples and processing delay, similar to conditions applied in some large, population-based studies, may affect measured perfluoroalkyl acid concentrations, mainly when outdoor temperatures are low. Attention to processing conditions is needed in studies of perfluoroalkyl acid exposure in humans. Public Library of Science 2015-09-10 /pmc/articles/PMC4565678/ /pubmed/26356420 http://dx.doi.org/10.1371/journal.pone.0137768 Text en © 2015 Bach et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bach, Cathrine Carlsen
Henriksen, Tine Brink
Bossi, Rossana
Bech, Bodil Hammer
Fuglsang, Jens
Olsen, Jørn
Nohr, Ellen Aagaard
Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay
title Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay
title_full Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay
title_fullStr Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay
title_full_unstemmed Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay
title_short Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay
title_sort perfluoroalkyl acid concentrations in blood samples subjected to transportation and processing delay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565678/
https://www.ncbi.nlm.nih.gov/pubmed/26356420
http://dx.doi.org/10.1371/journal.pone.0137768
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