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Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay
BACKGROUND: In studies of perfluoroalkyl acids, the validity and comparability of measured concentrations may be affected by differences in the handling of biospecimens. We aimed to investigate whether measured plasma levels of perfluoroalkyl acids differed between blood samples subjected to delay a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565678/ https://www.ncbi.nlm.nih.gov/pubmed/26356420 http://dx.doi.org/10.1371/journal.pone.0137768 |
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author | Bach, Cathrine Carlsen Henriksen, Tine Brink Bossi, Rossana Bech, Bodil Hammer Fuglsang, Jens Olsen, Jørn Nohr, Ellen Aagaard |
author_facet | Bach, Cathrine Carlsen Henriksen, Tine Brink Bossi, Rossana Bech, Bodil Hammer Fuglsang, Jens Olsen, Jørn Nohr, Ellen Aagaard |
author_sort | Bach, Cathrine Carlsen |
collection | PubMed |
description | BACKGROUND: In studies of perfluoroalkyl acids, the validity and comparability of measured concentrations may be affected by differences in the handling of biospecimens. We aimed to investigate whether measured plasma levels of perfluoroalkyl acids differed between blood samples subjected to delay and transportation prior to processing and samples with immediate processing and freezing. METHODS: Pregnant women recruited at Aarhus University Hospital, Denmark, (n = 88) provided paired blood samples. For each pair of samples, one was immediately processed and plasma was frozen, and the other was delayed and transported as whole blood before processing and freezing of plasma (similar to the Danish National Birth Cohort). We measured 12 perfluoroalkyl acids and present results for compounds with more than 50% of samples above the lower limit of quantification. RESULTS: For samples taken in the winter, relative differences between the paired samples ranged between -77 and +38% for individual perfluoroalkyl acids. In most cases concentrations were lower in the delayed and transported samples, e.g. the relative difference was -29% (95% confidence interval -30; -27) for perfluorooctane sulfonate. For perfluorooctanoate there was no difference between the two setups [corresponding estimate 1% (0, 3)]. Differences were negligible in the summer for all compounds. CONCLUSIONS: Transport of blood samples and processing delay, similar to conditions applied in some large, population-based studies, may affect measured perfluoroalkyl acid concentrations, mainly when outdoor temperatures are low. Attention to processing conditions is needed in studies of perfluoroalkyl acid exposure in humans. |
format | Online Article Text |
id | pubmed-4565678 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45656782015-09-18 Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay Bach, Cathrine Carlsen Henriksen, Tine Brink Bossi, Rossana Bech, Bodil Hammer Fuglsang, Jens Olsen, Jørn Nohr, Ellen Aagaard PLoS One Research Article BACKGROUND: In studies of perfluoroalkyl acids, the validity and comparability of measured concentrations may be affected by differences in the handling of biospecimens. We aimed to investigate whether measured plasma levels of perfluoroalkyl acids differed between blood samples subjected to delay and transportation prior to processing and samples with immediate processing and freezing. METHODS: Pregnant women recruited at Aarhus University Hospital, Denmark, (n = 88) provided paired blood samples. For each pair of samples, one was immediately processed and plasma was frozen, and the other was delayed and transported as whole blood before processing and freezing of plasma (similar to the Danish National Birth Cohort). We measured 12 perfluoroalkyl acids and present results for compounds with more than 50% of samples above the lower limit of quantification. RESULTS: For samples taken in the winter, relative differences between the paired samples ranged between -77 and +38% for individual perfluoroalkyl acids. In most cases concentrations were lower in the delayed and transported samples, e.g. the relative difference was -29% (95% confidence interval -30; -27) for perfluorooctane sulfonate. For perfluorooctanoate there was no difference between the two setups [corresponding estimate 1% (0, 3)]. Differences were negligible in the summer for all compounds. CONCLUSIONS: Transport of blood samples and processing delay, similar to conditions applied in some large, population-based studies, may affect measured perfluoroalkyl acid concentrations, mainly when outdoor temperatures are low. Attention to processing conditions is needed in studies of perfluoroalkyl acid exposure in humans. Public Library of Science 2015-09-10 /pmc/articles/PMC4565678/ /pubmed/26356420 http://dx.doi.org/10.1371/journal.pone.0137768 Text en © 2015 Bach et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bach, Cathrine Carlsen Henriksen, Tine Brink Bossi, Rossana Bech, Bodil Hammer Fuglsang, Jens Olsen, Jørn Nohr, Ellen Aagaard Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay |
title | Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay |
title_full | Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay |
title_fullStr | Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay |
title_full_unstemmed | Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay |
title_short | Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay |
title_sort | perfluoroalkyl acid concentrations in blood samples subjected to transportation and processing delay |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565678/ https://www.ncbi.nlm.nih.gov/pubmed/26356420 http://dx.doi.org/10.1371/journal.pone.0137768 |
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