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Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus

Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrop...

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Autores principales: AIHARA, Naoyuki, HORIUCHI, Noriyuki, HIKICHI, Nanase, OCHIAI, Mariko, HOSODA, Yuko, ISHIKAWA, Yoko, SHIMAZAKI, Yoko, OISHI, Koji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565812/
https://www.ncbi.nlm.nih.gov/pubmed/25866403
http://dx.doi.org/10.1292/jvms.14-0599
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author AIHARA, Naoyuki
HORIUCHI, Noriyuki
HIKICHI, Nanase
OCHIAI, Mariko
HOSODA, Yuko
ISHIKAWA, Yoko
SHIMAZAKI, Yoko
OISHI, Koji
author_facet AIHARA, Naoyuki
HORIUCHI, Noriyuki
HIKICHI, Nanase
OCHIAI, Mariko
HOSODA, Yuko
ISHIKAWA, Yoko
SHIMAZAKI, Yoko
OISHI, Koji
author_sort AIHARA, Naoyuki
collection PubMed
description Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine strain-administered groups (vaccine groups), and the level of severity was the same as that in the wild-type strain-administered group (wild-type group). However, these chickens retained humoral antibody responses to NDV and were revealed to possess a higher number of bursal follicles than those of the wild-type group. These results indicated that macroscopic evaluation dose not accurately reflect the immunoreactivity and degree of bursal damage in IBDV-administered chickens. We also found non-immunosuppressed chickens in the wild-type group. These non-immunosuppressed chickens retained a significantly higher number of normal follicles and total follicles according to our statistical analysis. Furthermore, a high correlation coefficient between the NDV-HI titer and the number of normal follicles was found in the wild-type group. These results implied that the retained number of normal follicles is important for the immunoreactivity of chickens infected with IBDV.
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spelling pubmed-45658122015-09-11 Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus AIHARA, Naoyuki HORIUCHI, Noriyuki HIKICHI, Nanase OCHIAI, Mariko HOSODA, Yuko ISHIKAWA, Yoko SHIMAZAKI, Yoko OISHI, Koji J Vet Med Sci Avian Pathology Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine strain-administered groups (vaccine groups), and the level of severity was the same as that in the wild-type strain-administered group (wild-type group). However, these chickens retained humoral antibody responses to NDV and were revealed to possess a higher number of bursal follicles than those of the wild-type group. These results indicated that macroscopic evaluation dose not accurately reflect the immunoreactivity and degree of bursal damage in IBDV-administered chickens. We also found non-immunosuppressed chickens in the wild-type group. These non-immunosuppressed chickens retained a significantly higher number of normal follicles and total follicles according to our statistical analysis. Furthermore, a high correlation coefficient between the NDV-HI titer and the number of normal follicles was found in the wild-type group. These results implied that the retained number of normal follicles is important for the immunoreactivity of chickens infected with IBDV. The Japanese Society of Veterinary Science 2015-04-10 2015-08 /pmc/articles/PMC4565812/ /pubmed/25866403 http://dx.doi.org/10.1292/jvms.14-0599 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Avian Pathology
AIHARA, Naoyuki
HORIUCHI, Noriyuki
HIKICHI, Nanase
OCHIAI, Mariko
HOSODA, Yuko
ISHIKAWA, Yoko
SHIMAZAKI, Yoko
OISHI, Koji
Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus
title Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus
title_full Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus
title_fullStr Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus
title_full_unstemmed Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus
title_short Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus
title_sort immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus
topic Avian Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565812/
https://www.ncbi.nlm.nih.gov/pubmed/25866403
http://dx.doi.org/10.1292/jvms.14-0599
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