A globotetraosylceramide (Gb(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e

Currently, no simple assays are available for routine quantitative detection of Escherichia coli-produced Shiga toxin 2e (Stx2e) that causes porcine edema disease. Here, we present a novel quantitative detection method for Stx2e based on the measurement of Stx2e binding to the specific globotetraosy...

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Autores principales: TOGASHI, Katsuhiro, SASAKI, Shiho, SATO, Wataru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565821/
https://www.ncbi.nlm.nih.gov/pubmed/25787850
http://dx.doi.org/10.1292/jvms.14-0071
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author TOGASHI, Katsuhiro
SASAKI, Shiho
SATO, Wataru
author_facet TOGASHI, Katsuhiro
SASAKI, Shiho
SATO, Wataru
author_sort TOGASHI, Katsuhiro
collection PubMed
description Currently, no simple assays are available for routine quantitative detection of Escherichia coli-produced Shiga toxin 2e (Stx2e) that causes porcine edema disease. Here, we present a novel quantitative detection method for Stx2e based on the measurement of Stx2e binding to the specific globotetraosylceramide (Gb(4)) receptor by ELISA (Gb(4)-ELISA). No cross-reactivity was found with the other Shiga toxins Stx1 and Stx2, indicating high specificity. When the recombinant Stx2e B subunit (Stx2eB) was used, the absorbance measured by Gb(4)-ELISA increased linearly with Stx2eB concentration in the range of 20–2,500 ng/ml. The Gb(4)-ELISA method can be easily performed, suggesting that it would be a useful diagnostic tool for porcine edema disease.
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spelling pubmed-45658212015-09-11 A globotetraosylceramide (Gb(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e TOGASHI, Katsuhiro SASAKI, Shiho SATO, Wataru J Vet Med Sci Bacteriology Currently, no simple assays are available for routine quantitative detection of Escherichia coli-produced Shiga toxin 2e (Stx2e) that causes porcine edema disease. Here, we present a novel quantitative detection method for Stx2e based on the measurement of Stx2e binding to the specific globotetraosylceramide (Gb(4)) receptor by ELISA (Gb(4)-ELISA). No cross-reactivity was found with the other Shiga toxins Stx1 and Stx2, indicating high specificity. When the recombinant Stx2e B subunit (Stx2eB) was used, the absorbance measured by Gb(4)-ELISA increased linearly with Stx2eB concentration in the range of 20–2,500 ng/ml. The Gb(4)-ELISA method can be easily performed, suggesting that it would be a useful diagnostic tool for porcine edema disease. The Japanese Society of Veterinary Science 2015-03-14 2015-08 /pmc/articles/PMC4565821/ /pubmed/25787850 http://dx.doi.org/10.1292/jvms.14-0071 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Bacteriology
TOGASHI, Katsuhiro
SASAKI, Shiho
SATO, Wataru
A globotetraosylceramide (Gb(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e
title A globotetraosylceramide (Gb(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e
title_full A globotetraosylceramide (Gb(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e
title_fullStr A globotetraosylceramide (Gb(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e
title_full_unstemmed A globotetraosylceramide (Gb(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e
title_short A globotetraosylceramide (Gb(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e
title_sort globotetraosylceramide (gb(4)) receptor-based elisa for quantitative detection of shiga toxin 2e
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565821/
https://www.ncbi.nlm.nih.gov/pubmed/25787850
http://dx.doi.org/10.1292/jvms.14-0071
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