Cargando…
Staurosporine Induced Apoptosis May Activate Cancer Stem-Like Cells (CD44(+)/CD24(-)) in MCF-7 by Upregulating Mucin1 and EpCAM
Malignant tumors recur after chemotherapy. A small population of cancer stem-like cells within tumors is now generally considered the prime source of the recurrence. To better understand how cancer stem-like cells induce relapse after fractionated chemotherapy, we examined changes in the CD44(+)/CD2...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565855/ https://www.ncbi.nlm.nih.gov/pubmed/26366219 http://dx.doi.org/10.7150/jca.12501 |
_version_ | 1782389633366097920 |
---|---|
author | Zhou, Na Wang, Rong Zhang, Yizhuang Lei, Zhen Zhang, Xuehui Hu, Ruobi Li, Hui Mao, Yiqing Wang, Xi Irwin, David M. Niu, Gang Tan, Huanran |
author_facet | Zhou, Na Wang, Rong Zhang, Yizhuang Lei, Zhen Zhang, Xuehui Hu, Ruobi Li, Hui Mao, Yiqing Wang, Xi Irwin, David M. Niu, Gang Tan, Huanran |
author_sort | Zhou, Na |
collection | PubMed |
description | Malignant tumors recur after chemotherapy. A small population of cancer stem-like cells within tumors is now generally considered the prime source of the recurrence. To better understand how cancer stem-like cells induce relapse after fractionated chemotherapy, we examined changes in the CD44(+)/CD24(-) cancer stem-like cells population and behavior using the breast cancer cell line MCF-7. Our results show that apart from an increase in the CD44(+)/CD24(-) population, proliferation and clone formation, but not migration, were enhanced after recovery from apoptosis induced by two pulses of staurosporine (STS). The distribution of cells in the cell cycle differed between acutely induced apoptosis and fractionated chemotherapy. Sorted CD44(+)/CD24(-) stem-like cells from MCF-7 cells recovered from STS treatment possessed greater proliferation abilities. We also observed that mucin1 (MUC1) and Epithelial cell adhesion molecule (EpCAM) were up-regulated in abundance coincidently with proliferation and clone formation enhancement. Our findings suggest that fractionated chemotherapy induced apoptosis could stimulate cancer stem-like cell to behave with a stronger malignant property than cancer cells themselves and MUC1 and EpCAM are important factors involving in this process. By demonstrating changes in cancer stem cell during chemotherapy and identifying the crucial factors, we potentially can target them, to eradicate tumors and overcome cancer relapse. |
format | Online Article Text |
id | pubmed-4565855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-45658552015-09-11 Staurosporine Induced Apoptosis May Activate Cancer Stem-Like Cells (CD44(+)/CD24(-)) in MCF-7 by Upregulating Mucin1 and EpCAM Zhou, Na Wang, Rong Zhang, Yizhuang Lei, Zhen Zhang, Xuehui Hu, Ruobi Li, Hui Mao, Yiqing Wang, Xi Irwin, David M. Niu, Gang Tan, Huanran J Cancer Research Paper Malignant tumors recur after chemotherapy. A small population of cancer stem-like cells within tumors is now generally considered the prime source of the recurrence. To better understand how cancer stem-like cells induce relapse after fractionated chemotherapy, we examined changes in the CD44(+)/CD24(-) cancer stem-like cells population and behavior using the breast cancer cell line MCF-7. Our results show that apart from an increase in the CD44(+)/CD24(-) population, proliferation and clone formation, but not migration, were enhanced after recovery from apoptosis induced by two pulses of staurosporine (STS). The distribution of cells in the cell cycle differed between acutely induced apoptosis and fractionated chemotherapy. Sorted CD44(+)/CD24(-) stem-like cells from MCF-7 cells recovered from STS treatment possessed greater proliferation abilities. We also observed that mucin1 (MUC1) and Epithelial cell adhesion molecule (EpCAM) were up-regulated in abundance coincidently with proliferation and clone formation enhancement. Our findings suggest that fractionated chemotherapy induced apoptosis could stimulate cancer stem-like cell to behave with a stronger malignant property than cancer cells themselves and MUC1 and EpCAM are important factors involving in this process. By demonstrating changes in cancer stem cell during chemotherapy and identifying the crucial factors, we potentially can target them, to eradicate tumors and overcome cancer relapse. Ivyspring International Publisher 2015-09-01 /pmc/articles/PMC4565855/ /pubmed/26366219 http://dx.doi.org/10.7150/jca.12501 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Research Paper Zhou, Na Wang, Rong Zhang, Yizhuang Lei, Zhen Zhang, Xuehui Hu, Ruobi Li, Hui Mao, Yiqing Wang, Xi Irwin, David M. Niu, Gang Tan, Huanran Staurosporine Induced Apoptosis May Activate Cancer Stem-Like Cells (CD44(+)/CD24(-)) in MCF-7 by Upregulating Mucin1 and EpCAM |
title | Staurosporine Induced Apoptosis May Activate Cancer Stem-Like Cells (CD44(+)/CD24(-)) in MCF-7 by Upregulating Mucin1 and EpCAM |
title_full | Staurosporine Induced Apoptosis May Activate Cancer Stem-Like Cells (CD44(+)/CD24(-)) in MCF-7 by Upregulating Mucin1 and EpCAM |
title_fullStr | Staurosporine Induced Apoptosis May Activate Cancer Stem-Like Cells (CD44(+)/CD24(-)) in MCF-7 by Upregulating Mucin1 and EpCAM |
title_full_unstemmed | Staurosporine Induced Apoptosis May Activate Cancer Stem-Like Cells (CD44(+)/CD24(-)) in MCF-7 by Upregulating Mucin1 and EpCAM |
title_short | Staurosporine Induced Apoptosis May Activate Cancer Stem-Like Cells (CD44(+)/CD24(-)) in MCF-7 by Upregulating Mucin1 and EpCAM |
title_sort | staurosporine induced apoptosis may activate cancer stem-like cells (cd44(+)/cd24(-)) in mcf-7 by upregulating mucin1 and epcam |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565855/ https://www.ncbi.nlm.nih.gov/pubmed/26366219 http://dx.doi.org/10.7150/jca.12501 |
work_keys_str_mv | AT zhouna staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT wangrong staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT zhangyizhuang staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT leizhen staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT zhangxuehui staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT huruobi staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT lihui staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT maoyiqing staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT wangxi staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT irwindavidm staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT niugang staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam AT tanhuanran staurosporineinducedapoptosismayactivatecancerstemlikecellscd44cd24inmcf7byupregulatingmucin1andepcam |