Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli

OBJECTIVES: To use the glucose–fructose oxidoreductase (GFOR) from Zymomonas mobilis and expressed in Escherichia coli for lactobionic acid production by conversion of lactose from whey. RESULTS: The highest concentrations of lactobionic acid (3.2 mg ml(−1)) during oxidation of whey-derived lactose...

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Autores principales: Goderska, Kamila, Juzwa, Wojciech, Szwengiel, Artur, Czarnecki, Zbigniew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2015
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565883/
https://www.ncbi.nlm.nih.gov/pubmed/26091863
http://dx.doi.org/10.1007/s10529-015-1887-0
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author Goderska, Kamila
Juzwa, Wojciech
Szwengiel, Artur
Czarnecki, Zbigniew
author_facet Goderska, Kamila
Juzwa, Wojciech
Szwengiel, Artur
Czarnecki, Zbigniew
author_sort Goderska, Kamila
collection PubMed
description OBJECTIVES: To use the glucose–fructose oxidoreductase (GFOR) from Zymomonas mobilis and expressed in Escherichia coli for lactobionic acid production by conversion of lactose from whey. RESULTS: The highest concentrations of lactobionic acid (3.2 mg ml(−1)) during oxidation of whey-derived lactose by E. coli was at 24 h. Introduction of GFOR gene from Z. mobilis, into E. coli improved enzyme yields compared to what is obtainable by fermentation of the donor strain. The production of lactobionic acid by E. coli was 2.6-times higher than by Z. mobilis. CONCLUSIONS: Recombinants of E. coli overexpressing the GFOR gene from Z. mobilis produced higher amount of lactobionoic acid from whey-derived lactose.
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spelling pubmed-45658832015-09-15 Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli Goderska, Kamila Juzwa, Wojciech Szwengiel, Artur Czarnecki, Zbigniew Biotechnol Lett Original Research Paper OBJECTIVES: To use the glucose–fructose oxidoreductase (GFOR) from Zymomonas mobilis and expressed in Escherichia coli for lactobionic acid production by conversion of lactose from whey. RESULTS: The highest concentrations of lactobionic acid (3.2 mg ml(−1)) during oxidation of whey-derived lactose by E. coli was at 24 h. Introduction of GFOR gene from Z. mobilis, into E. coli improved enzyme yields compared to what is obtainable by fermentation of the donor strain. The production of lactobionic acid by E. coli was 2.6-times higher than by Z. mobilis. CONCLUSIONS: Recombinants of E. coli overexpressing the GFOR gene from Z. mobilis produced higher amount of lactobionoic acid from whey-derived lactose. Springer Netherlands 2015-06-20 2015 /pmc/articles/PMC4565883/ /pubmed/26091863 http://dx.doi.org/10.1007/s10529-015-1887-0 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Research Paper
Goderska, Kamila
Juzwa, Wojciech
Szwengiel, Artur
Czarnecki, Zbigniew
Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
title Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
title_full Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
title_fullStr Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
title_full_unstemmed Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
title_short Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
title_sort lactobionic acid production by glucose–fructose oxidoreductase from zymomonas mobilis expressed in escherichia coli
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565883/
https://www.ncbi.nlm.nih.gov/pubmed/26091863
http://dx.doi.org/10.1007/s10529-015-1887-0
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AT szwengielartur lactobionicacidproductionbyglucosefructoseoxidoreductasefromzymomonasmobilisexpressedinescherichiacoli
AT czarneckizbigniew lactobionicacidproductionbyglucosefructoseoxidoreductasefromzymomonasmobilisexpressedinescherichiacoli

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