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Screening for recombinants of Crambe abyssynica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration
The T-DNA region of pMF1 vector of marker-free system developed by Wageningen UR, has Recombinase R-LBD gene fusion and nptII and codA gene fusion between two recombination sites. After transformation applying dexamethasone (DEX) can activate the recombinase to remove the T-DNA fragment between reco...
Autores principales: | Qi, Weicong, Tinnenbroek-Capel, Iris E. M., Salentijn, Elma M. J., Schaart, Jan G., Cheng, Jihua, Denneboom, Christel, Zhang, Zhao, Zhang, Xiaolin, Zhao, Han, Visser, Richard G. F., Huang, Bangquan, Van Loo, Eibertus N., Krens, Frans A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4566089/ https://www.ncbi.nlm.nih.gov/pubmed/26358007 http://dx.doi.org/10.1038/srep14033 |
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