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High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes

Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based expression of Cas9 and the gene-specific guide RNAs. Here we report that direct injection of in vitro–ass...

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Detalles Bibliográficos
Autores principales:	Paix, Alexandre, Folkmann, Andrew, Rasoloson, Dominique, Seydoux, Geraldine
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2015
Materias:	Investigations
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4566275/ https://www.ncbi.nlm.nih.gov/pubmed/26187122 http://dx.doi.org/10.1534/genetics.115.179382

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