Cargando…
Effects of prostaglandin E1 on callus formation in rabbits
BACKGROUND: Recent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model. METHODS: Data were collected on callus formation in 14 male rabbits. These were divided in...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4566397/ https://www.ncbi.nlm.nih.gov/pubmed/26359236 http://dx.doi.org/10.1186/s12891-015-0695-y |
_version_ | 1782389713100865536 |
---|---|
author | Lipinsky, Pawel V. Sirotin, Ivan V. Skoroglyadov, Alexandr V. Ivkov, Alexey V. Oettinger, Alexandr P. Krynetskiy, Evgeny E. But-Gusaim, Alexandr B. Roth, Andreas J. |
author_facet | Lipinsky, Pawel V. Sirotin, Ivan V. Skoroglyadov, Alexandr V. Ivkov, Alexey V. Oettinger, Alexandr P. Krynetskiy, Evgeny E. But-Gusaim, Alexandr B. Roth, Andreas J. |
author_sort | Lipinsky, Pawel V. |
collection | PubMed |
description | BACKGROUND: Recent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model. METHODS: Data were collected on callus formation in 14 male rabbits. These were divided into two groups (control and treatment) with 7 animals in each group. In all animals, the right tibia was fractured using a standardized protocol and stabilized by an intramedullary nail. Treatment group received a 5 μg/kg subcutaneous injection of PGE1/day during 10 postoperative days. Visual and radiological evaluation of callus formation was prospectively collected. After 30 days, all animals were killed and the tibia specimens were examined histologically. RESULTS: In all the treatment group animals, fractures were consolidated radiologically by day 30. No treatment group animals and two control group animals were excluded form the experiment. In the control group, 4 animals demonstrated slower callus formation than the main group. Two control group animals were excluded from the experiment on the 20th day due to wound infection; one developed a nonunion. The mean coefficient of bone callus thickening in the main group was 2.08 (±0, 16) and 1.77 (±0.05) (p < 0.05) in the control group. Calculation of mean quantity of neogenic vessels in 10 random visual fields of the bone callus revealed 78 (±9.82) in the main group and 40 (±4.68) in the control group (p < 0.05). CONCLUSIONS: Our study demonstrates an increased rate and amount of bone callus formation in the group treated with prostaglandin E1 compared to the control group. Prospective radiological analysis was corroborated by histologic evaluation. |
format | Online Article Text |
id | pubmed-4566397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45663972015-09-12 Effects of prostaglandin E1 on callus formation in rabbits Lipinsky, Pawel V. Sirotin, Ivan V. Skoroglyadov, Alexandr V. Ivkov, Alexey V. Oettinger, Alexandr P. Krynetskiy, Evgeny E. But-Gusaim, Alexandr B. Roth, Andreas J. BMC Musculoskelet Disord Research Article BACKGROUND: Recent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model. METHODS: Data were collected on callus formation in 14 male rabbits. These were divided into two groups (control and treatment) with 7 animals in each group. In all animals, the right tibia was fractured using a standardized protocol and stabilized by an intramedullary nail. Treatment group received a 5 μg/kg subcutaneous injection of PGE1/day during 10 postoperative days. Visual and radiological evaluation of callus formation was prospectively collected. After 30 days, all animals were killed and the tibia specimens were examined histologically. RESULTS: In all the treatment group animals, fractures were consolidated radiologically by day 30. No treatment group animals and two control group animals were excluded form the experiment. In the control group, 4 animals demonstrated slower callus formation than the main group. Two control group animals were excluded from the experiment on the 20th day due to wound infection; one developed a nonunion. The mean coefficient of bone callus thickening in the main group was 2.08 (±0, 16) and 1.77 (±0.05) (p < 0.05) in the control group. Calculation of mean quantity of neogenic vessels in 10 random visual fields of the bone callus revealed 78 (±9.82) in the main group and 40 (±4.68) in the control group (p < 0.05). CONCLUSIONS: Our study demonstrates an increased rate and amount of bone callus formation in the group treated with prostaglandin E1 compared to the control group. Prospective radiological analysis was corroborated by histologic evaluation. BioMed Central 2015-09-10 /pmc/articles/PMC4566397/ /pubmed/26359236 http://dx.doi.org/10.1186/s12891-015-0695-y Text en © Lipinsky et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Lipinsky, Pawel V. Sirotin, Ivan V. Skoroglyadov, Alexandr V. Ivkov, Alexey V. Oettinger, Alexandr P. Krynetskiy, Evgeny E. But-Gusaim, Alexandr B. Roth, Andreas J. Effects of prostaglandin E1 on callus formation in rabbits |
title | Effects of prostaglandin E1 on callus formation in rabbits |
title_full | Effects of prostaglandin E1 on callus formation in rabbits |
title_fullStr | Effects of prostaglandin E1 on callus formation in rabbits |
title_full_unstemmed | Effects of prostaglandin E1 on callus formation in rabbits |
title_short | Effects of prostaglandin E1 on callus formation in rabbits |
title_sort | effects of prostaglandin e1 on callus formation in rabbits |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4566397/ https://www.ncbi.nlm.nih.gov/pubmed/26359236 http://dx.doi.org/10.1186/s12891-015-0695-y |
work_keys_str_mv | AT lipinskypawelv effectsofprostaglandine1oncallusformationinrabbits AT sirotinivanv effectsofprostaglandine1oncallusformationinrabbits AT skoroglyadovalexandrv effectsofprostaglandine1oncallusformationinrabbits AT ivkovalexeyv effectsofprostaglandine1oncallusformationinrabbits AT oettingeralexandrp effectsofprostaglandine1oncallusformationinrabbits AT krynetskiyevgenye effectsofprostaglandine1oncallusformationinrabbits AT butgusaimalexandrb effectsofprostaglandine1oncallusformationinrabbits AT rothandreasj effectsofprostaglandine1oncallusformationinrabbits |