Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms

This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immun...

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Detalles Bibliográficos
Autores principales: Kian, Danielle, Lancheros, César Armando Contreras, Damiani, Igor Alexandre Campos, Fernandes, Tamiris Zanforlin Olmos, Pinge-Filho, Phileno, dos Santos, Márcia Regina Machado, da Silveira, José Franco, Nakamura, Celso Vataru, da Silva, João Santana, Yamada-Ogatta, Sueli Fumie, Yamauchi, Lucy Megumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2015
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4566500/
https://www.ncbi.nlm.nih.gov/pubmed/26323848
http://dx.doi.org/10.3347/kjp.2015.53.4.483
Descripción
Sumario:This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.

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