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An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments

During metastasis, cancerous cells leave the primary tumour, pass into the circulatory system, and invade into new tissues. To migrate through the wide variety of environments they encounter, the cells must be able to remodel their cell shape efficiently to squeeze through small gaps in the extracel...

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Detalles Bibliográficos
Autores principales: Malboubi, Majid, Jayo, Asier, Parsons, Maddy, Charras, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567073/
https://www.ncbi.nlm.nih.gov/pubmed/26412914
http://dx.doi.org/10.1016/j.mee.2015.02.022
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author Malboubi, Majid
Jayo, Asier
Parsons, Maddy
Charras, Guillaume
author_facet Malboubi, Majid
Jayo, Asier
Parsons, Maddy
Charras, Guillaume
author_sort Malboubi, Majid
collection PubMed
description During metastasis, cancerous cells leave the primary tumour, pass into the circulatory system, and invade into new tissues. To migrate through the wide variety of environments they encounter, the cells must be able to remodel their cell shape efficiently to squeeze through small gaps in the extracellular matrix or extravasate into the blood stream or lymphatic system. Several studies have shown that the nucleus is the main limiting factor to migration through small gaps (Wolf et al., 2013; Harada et al., 2014; Mak et al., 2013). To understand the physical limits of cancer cell translocation in confined environments, we have fabricated a microfluidic device to study their ability to adapt their nuclear and cellular shape when passing through small gaps. The device is open access for ease of use and enables examination of the effect of different levels of spatial confinement on cell behaviour and morphology simultaneously. The results show that increasing cell confinement decreases the ability of cells to translocate into small gaps and that cells cannot penetrate into the microchannels below a threshold cross-section.
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spelling pubmed-45670732015-09-25 An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments Malboubi, Majid Jayo, Asier Parsons, Maddy Charras, Guillaume Microelectron Eng Article During metastasis, cancerous cells leave the primary tumour, pass into the circulatory system, and invade into new tissues. To migrate through the wide variety of environments they encounter, the cells must be able to remodel their cell shape efficiently to squeeze through small gaps in the extracellular matrix or extravasate into the blood stream or lymphatic system. Several studies have shown that the nucleus is the main limiting factor to migration through small gaps (Wolf et al., 2013; Harada et al., 2014; Mak et al., 2013). To understand the physical limits of cancer cell translocation in confined environments, we have fabricated a microfluidic device to study their ability to adapt their nuclear and cellular shape when passing through small gaps. The device is open access for ease of use and enables examination of the effect of different levels of spatial confinement on cell behaviour and morphology simultaneously. The results show that increasing cell confinement decreases the ability of cells to translocate into small gaps and that cells cannot penetrate into the microchannels below a threshold cross-section. Elsevier 2015-08-16 /pmc/articles/PMC4567073/ /pubmed/26412914 http://dx.doi.org/10.1016/j.mee.2015.02.022 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malboubi, Majid
Jayo, Asier
Parsons, Maddy
Charras, Guillaume
An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments
title An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments
title_full An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments
title_fullStr An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments
title_full_unstemmed An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments
title_short An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments
title_sort open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567073/
https://www.ncbi.nlm.nih.gov/pubmed/26412914
http://dx.doi.org/10.1016/j.mee.2015.02.022
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