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An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments
During metastasis, cancerous cells leave the primary tumour, pass into the circulatory system, and invade into new tissues. To migrate through the wide variety of environments they encounter, the cells must be able to remodel their cell shape efficiently to squeeze through small gaps in the extracel...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567073/ https://www.ncbi.nlm.nih.gov/pubmed/26412914 http://dx.doi.org/10.1016/j.mee.2015.02.022 |
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author | Malboubi, Majid Jayo, Asier Parsons, Maddy Charras, Guillaume |
author_facet | Malboubi, Majid Jayo, Asier Parsons, Maddy Charras, Guillaume |
author_sort | Malboubi, Majid |
collection | PubMed |
description | During metastasis, cancerous cells leave the primary tumour, pass into the circulatory system, and invade into new tissues. To migrate through the wide variety of environments they encounter, the cells must be able to remodel their cell shape efficiently to squeeze through small gaps in the extracellular matrix or extravasate into the blood stream or lymphatic system. Several studies have shown that the nucleus is the main limiting factor to migration through small gaps (Wolf et al., 2013; Harada et al., 2014; Mak et al., 2013). To understand the physical limits of cancer cell translocation in confined environments, we have fabricated a microfluidic device to study their ability to adapt their nuclear and cellular shape when passing through small gaps. The device is open access for ease of use and enables examination of the effect of different levels of spatial confinement on cell behaviour and morphology simultaneously. The results show that increasing cell confinement decreases the ability of cells to translocate into small gaps and that cells cannot penetrate into the microchannels below a threshold cross-section. |
format | Online Article Text |
id | pubmed-4567073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-45670732015-09-25 An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments Malboubi, Majid Jayo, Asier Parsons, Maddy Charras, Guillaume Microelectron Eng Article During metastasis, cancerous cells leave the primary tumour, pass into the circulatory system, and invade into new tissues. To migrate through the wide variety of environments they encounter, the cells must be able to remodel their cell shape efficiently to squeeze through small gaps in the extracellular matrix or extravasate into the blood stream or lymphatic system. Several studies have shown that the nucleus is the main limiting factor to migration through small gaps (Wolf et al., 2013; Harada et al., 2014; Mak et al., 2013). To understand the physical limits of cancer cell translocation in confined environments, we have fabricated a microfluidic device to study their ability to adapt their nuclear and cellular shape when passing through small gaps. The device is open access for ease of use and enables examination of the effect of different levels of spatial confinement on cell behaviour and morphology simultaneously. The results show that increasing cell confinement decreases the ability of cells to translocate into small gaps and that cells cannot penetrate into the microchannels below a threshold cross-section. Elsevier 2015-08-16 /pmc/articles/PMC4567073/ /pubmed/26412914 http://dx.doi.org/10.1016/j.mee.2015.02.022 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Malboubi, Majid Jayo, Asier Parsons, Maddy Charras, Guillaume An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments |
title | An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments |
title_full | An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments |
title_fullStr | An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments |
title_full_unstemmed | An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments |
title_short | An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments |
title_sort | open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567073/ https://www.ncbi.nlm.nih.gov/pubmed/26412914 http://dx.doi.org/10.1016/j.mee.2015.02.022 |
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