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Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone
The design of hybrid poly-ε-caprolactone (PCL)-self-assembling peptides (SAPs) matrices represents a simple method for the surface functionalization of synthetic scaffolds, which is essential for cell compatibility. This study investigates the influence of increasing concentrations (2.5%, 5%, 10% an...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567138/ https://www.ncbi.nlm.nih.gov/pubmed/26361004 http://dx.doi.org/10.1371/journal.pone.0137505 |
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author | Dettin, Monica Zamuner, Annj Roso, Martina Gloria, Antonio Iucci, Giovanna Messina, Grazia M. L. D'Amora, Ugo Marletta, Giovanni Modesti, Michele Castagliuolo, Ignazio Brun, Paola |
author_facet | Dettin, Monica Zamuner, Annj Roso, Martina Gloria, Antonio Iucci, Giovanna Messina, Grazia M. L. D'Amora, Ugo Marletta, Giovanni Modesti, Michele Castagliuolo, Ignazio Brun, Paola |
author_sort | Dettin, Monica |
collection | PubMed |
description | The design of hybrid poly-ε-caprolactone (PCL)-self-assembling peptides (SAPs) matrices represents a simple method for the surface functionalization of synthetic scaffolds, which is essential for cell compatibility. This study investigates the influence of increasing concentrations (2.5%, 5%, 10% and 15% w/w SAP compared to PCL) of three different SAPs on the physico-chemical/mechanical and biological properties of PCL fibers. We demonstrated that physico-chemical surface characteristics were slightly improved at increasing SAP concentrations: the fiber diameter increased; surface wettability increased with the first SAP addition (2.5%) and slightly less for the following ones; SAP-surface density increased but no change in the conformation was registered. These results could allow engineering matrices with structural characteristics and desired wettability according to the needs and the cell system used. The biological and mechanical characteristics of these scaffolds showed a particular trend at increasing SAP concentrations suggesting a prevailing correlation between cell behavior and mechanical features of the matrices. As compared with bare PCL, SAP enrichment increased the number of metabolic active h-osteoblast cells, fostered the expression of specific osteoblast-related mRNA transcripts, and guided calcium deposition, revealing the potential application of PCL-SAP scaffolds for the maintenance of osteoblast phenotype. |
format | Online Article Text |
id | pubmed-4567138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45671382015-09-18 Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone Dettin, Monica Zamuner, Annj Roso, Martina Gloria, Antonio Iucci, Giovanna Messina, Grazia M. L. D'Amora, Ugo Marletta, Giovanni Modesti, Michele Castagliuolo, Ignazio Brun, Paola PLoS One Research Article The design of hybrid poly-ε-caprolactone (PCL)-self-assembling peptides (SAPs) matrices represents a simple method for the surface functionalization of synthetic scaffolds, which is essential for cell compatibility. This study investigates the influence of increasing concentrations (2.5%, 5%, 10% and 15% w/w SAP compared to PCL) of three different SAPs on the physico-chemical/mechanical and biological properties of PCL fibers. We demonstrated that physico-chemical surface characteristics were slightly improved at increasing SAP concentrations: the fiber diameter increased; surface wettability increased with the first SAP addition (2.5%) and slightly less for the following ones; SAP-surface density increased but no change in the conformation was registered. These results could allow engineering matrices with structural characteristics and desired wettability according to the needs and the cell system used. The biological and mechanical characteristics of these scaffolds showed a particular trend at increasing SAP concentrations suggesting a prevailing correlation between cell behavior and mechanical features of the matrices. As compared with bare PCL, SAP enrichment increased the number of metabolic active h-osteoblast cells, fostered the expression of specific osteoblast-related mRNA transcripts, and guided calcium deposition, revealing the potential application of PCL-SAP scaffolds for the maintenance of osteoblast phenotype. Public Library of Science 2015-09-11 /pmc/articles/PMC4567138/ /pubmed/26361004 http://dx.doi.org/10.1371/journal.pone.0137505 Text en © 2015 Dettin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Dettin, Monica Zamuner, Annj Roso, Martina Gloria, Antonio Iucci, Giovanna Messina, Grazia M. L. D'Amora, Ugo Marletta, Giovanni Modesti, Michele Castagliuolo, Ignazio Brun, Paola Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone |
title | Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone |
title_full | Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone |
title_fullStr | Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone |
title_full_unstemmed | Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone |
title_short | Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone |
title_sort | electrospun scaffolds for osteoblast cells: peptide-induced concentration-dependent improvements of polycaprolactone |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567138/ https://www.ncbi.nlm.nih.gov/pubmed/26361004 http://dx.doi.org/10.1371/journal.pone.0137505 |
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