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The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans

Orchestration of cellular growth and development occurs during the life cycle of Aspergillus nidulans. A multi-copy genetic screen intended to unveil novel regulators of development identified the AN6578 locus predicted to encode a protein with the WOPR domain, which is a broadly present fungi-speci...

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Autores principales: Alkahyyat, Fahad, Ni, Min, Kim, Sun Chang, Yu, Jae-Hyuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567300/
https://www.ncbi.nlm.nih.gov/pubmed/26359867
http://dx.doi.org/10.1371/journal.pone.0137554
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author Alkahyyat, Fahad
Ni, Min
Kim, Sun Chang
Yu, Jae-Hyuk
author_facet Alkahyyat, Fahad
Ni, Min
Kim, Sun Chang
Yu, Jae-Hyuk
author_sort Alkahyyat, Fahad
collection PubMed
description Orchestration of cellular growth and development occurs during the life cycle of Aspergillus nidulans. A multi-copy genetic screen intended to unveil novel regulators of development identified the AN6578 locus predicted to encode a protein with the WOPR domain, which is a broadly present fungi-specific DNA-binding motif. Multi-copy of AN6578 disrupted the normal life cycle of the fungus leading to enhanced proliferation of vegetative cells, whereas the deletion resulted in hyper-active sexual fruiting with reduced asexual development (conidiation), thus named as osaA (Orchestrator of Sex and Asex). Further genetic studies indicate that OsaA balances development mainly by repressing sexual development downstream of the velvet regulator VeA. The absence of osaA is sufficient to suppress the veA1 allele leading to the sporulation levels comparable to veA (+) wild type (WT). Genome-wide transcriptomic analyses of WT, veA1, and ΔosaA veA1 strains by RNA-Seq further corroborate that OsaA functions in repressing sexual development downstream of VeA. However, OsaA also plays additional roles in controlling development, as the ΔosaA veA1 mutant exhibits precocious and enhanced formation of Hülle cells compared to WT. The OsaA orthologue of Aspergillus flavus is able to complement the osaA null phenotype in A. nidulans, suggesting a conserved role of this group of WOPR domain proteins. In summary, OsaA is an upstream orchestrator of morphological and chemical development in Aspergillus that functions downstream of VeA.
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spelling pubmed-45673002015-09-18 The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans Alkahyyat, Fahad Ni, Min Kim, Sun Chang Yu, Jae-Hyuk PLoS One Research Article Orchestration of cellular growth and development occurs during the life cycle of Aspergillus nidulans. A multi-copy genetic screen intended to unveil novel regulators of development identified the AN6578 locus predicted to encode a protein with the WOPR domain, which is a broadly present fungi-specific DNA-binding motif. Multi-copy of AN6578 disrupted the normal life cycle of the fungus leading to enhanced proliferation of vegetative cells, whereas the deletion resulted in hyper-active sexual fruiting with reduced asexual development (conidiation), thus named as osaA (Orchestrator of Sex and Asex). Further genetic studies indicate that OsaA balances development mainly by repressing sexual development downstream of the velvet regulator VeA. The absence of osaA is sufficient to suppress the veA1 allele leading to the sporulation levels comparable to veA (+) wild type (WT). Genome-wide transcriptomic analyses of WT, veA1, and ΔosaA veA1 strains by RNA-Seq further corroborate that OsaA functions in repressing sexual development downstream of VeA. However, OsaA also plays additional roles in controlling development, as the ΔosaA veA1 mutant exhibits precocious and enhanced formation of Hülle cells compared to WT. The OsaA orthologue of Aspergillus flavus is able to complement the osaA null phenotype in A. nidulans, suggesting a conserved role of this group of WOPR domain proteins. In summary, OsaA is an upstream orchestrator of morphological and chemical development in Aspergillus that functions downstream of VeA. Public Library of Science 2015-09-11 /pmc/articles/PMC4567300/ /pubmed/26359867 http://dx.doi.org/10.1371/journal.pone.0137554 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Alkahyyat, Fahad
Ni, Min
Kim, Sun Chang
Yu, Jae-Hyuk
The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans
title The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans
title_full The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans
title_fullStr The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans
title_full_unstemmed The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans
title_short The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans
title_sort wopr domain protein osaa orchestrates development in aspergillus nidulans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567300/
https://www.ncbi.nlm.nih.gov/pubmed/26359867
http://dx.doi.org/10.1371/journal.pone.0137554
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