Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers

We designed a primer pair (BtubNomF/BtubNomR) specifically for amplifying Aspergillus nomius DNA. In vitro assays confirmed BtubNomF/BtubNomR specificity, corroborating its usefulness in detecting and identifying A. nomius. We then investigated the occurrence of A. nomius in floral visitors of Berth...

Descripción completa

Detalles Bibliográficos
Autores principales: Massi, Fernanda Pelisson, Penha, Rafael Elias Silva, Cavalcante, Marcelo Casimiro, Viaro, Helena Paula, da Silva, Josué José, de Souza Ferranti, Larissa, Fungaro, Maria Helena Pelegrinelli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567567/
https://www.ncbi.nlm.nih.gov/pubmed/26063353
http://dx.doi.org/10.1264/jsme2.ME14146
_version_ 1782389825498775552
author Massi, Fernanda Pelisson
Penha, Rafael Elias Silva
Cavalcante, Marcelo Casimiro
Viaro, Helena Paula
da Silva, Josué José
de Souza Ferranti, Larissa
Fungaro, Maria Helena Pelegrinelli
author_facet Massi, Fernanda Pelisson
Penha, Rafael Elias Silva
Cavalcante, Marcelo Casimiro
Viaro, Helena Paula
da Silva, Josué José
de Souza Ferranti, Larissa
Fungaro, Maria Helena Pelegrinelli
author_sort Massi, Fernanda Pelisson
collection PubMed
description We designed a primer pair (BtubNomF/BtubNomR) specifically for amplifying Aspergillus nomius DNA. In vitro assays confirmed BtubNomF/BtubNomR specificity, corroborating its usefulness in detecting and identifying A. nomius. We then investigated the occurrence of A. nomius in floral visitors of Bertholletia excelsa trees by means of PCR, and A. nomius was detected in the following bees: Xylocopa frontalis, Bombus transversalis, Centris denudans, C. ferruginea, and Epicharis flava. The presence of A. nomius in bees visiting Brazil nuts opens up new avenues for obtaining novel insights into the process whereby Brazil nuts are contaminated by aflatoxin-producing fungi.
format Online
Article
Text
id pubmed-4567567
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI)
record_format MEDLINE/PubMed
spelling pubmed-45675672015-09-15 Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers Massi, Fernanda Pelisson Penha, Rafael Elias Silva Cavalcante, Marcelo Casimiro Viaro, Helena Paula da Silva, Josué José de Souza Ferranti, Larissa Fungaro, Maria Helena Pelegrinelli Microbes Environ Short Communication We designed a primer pair (BtubNomF/BtubNomR) specifically for amplifying Aspergillus nomius DNA. In vitro assays confirmed BtubNomF/BtubNomR specificity, corroborating its usefulness in detecting and identifying A. nomius. We then investigated the occurrence of A. nomius in floral visitors of Bertholletia excelsa trees by means of PCR, and A. nomius was detected in the following bees: Xylocopa frontalis, Bombus transversalis, Centris denudans, C. ferruginea, and Epicharis flava. The presence of A. nomius in bees visiting Brazil nuts opens up new avenues for obtaining novel insights into the process whereby Brazil nuts are contaminated by aflatoxin-producing fungi. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2015-09 2015-06-09 /pmc/articles/PMC4567567/ /pubmed/26063353 http://dx.doi.org/10.1264/jsme2.ME14146 Text en Copyright © 2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Massi, Fernanda Pelisson
Penha, Rafael Elias Silva
Cavalcante, Marcelo Casimiro
Viaro, Helena Paula
da Silva, Josué José
de Souza Ferranti, Larissa
Fungaro, Maria Helena Pelegrinelli
Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers
title Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers
title_full Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers
title_fullStr Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers
title_full_unstemmed Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers
title_short Identification of Aspergillus nomius in Bees Visiting Brazil Nut Flowers
title_sort identification of aspergillus nomius in bees visiting brazil nut flowers
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567567/
https://www.ncbi.nlm.nih.gov/pubmed/26063353
http://dx.doi.org/10.1264/jsme2.ME14146
work_keys_str_mv AT massifernandapelisson identificationofaspergillusnomiusinbeesvisitingbrazilnutflowers
AT penharafaeleliassilva identificationofaspergillusnomiusinbeesvisitingbrazilnutflowers
AT cavalcantemarcelocasimiro identificationofaspergillusnomiusinbeesvisitingbrazilnutflowers
AT viarohelenapaula identificationofaspergillusnomiusinbeesvisitingbrazilnutflowers
AT dasilvajosuejose identificationofaspergillusnomiusinbeesvisitingbrazilnutflowers
AT desouzaferrantilarissa identificationofaspergillusnomiusinbeesvisitingbrazilnutflowers
AT fungaromariahelenapelegrinelli identificationofaspergillusnomiusinbeesvisitingbrazilnutflowers

Ejemplares similares