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Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice

BACKGROUND: Abscisic acid (ABA) plays crucial roles in regulating plant growth and development, especially in responding to abiotic stress. The pyrabactin resistance-like (PYL) abscisic acid receptor family has been identified and widely characterized in Arabidopsis. However, PYL families in rice we...

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Autores principales: Tian, Xiaojie, Wang, Zhenyu, Li, Xiufeng, Lv, Tianxiao, Liu, Huazhao, Wang, Lizhi, Niu, Hongbin, Bu, Qingyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567572/
https://www.ncbi.nlm.nih.gov/pubmed/26362328
http://dx.doi.org/10.1186/s12284-015-0061-6
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author Tian, Xiaojie
Wang, Zhenyu
Li, Xiufeng
Lv, Tianxiao
Liu, Huazhao
Wang, Lizhi
Niu, Hongbin
Bu, Qingyun
author_facet Tian, Xiaojie
Wang, Zhenyu
Li, Xiufeng
Lv, Tianxiao
Liu, Huazhao
Wang, Lizhi
Niu, Hongbin
Bu, Qingyun
author_sort Tian, Xiaojie
collection PubMed
description BACKGROUND: Abscisic acid (ABA) plays crucial roles in regulating plant growth and development, especially in responding to abiotic stress. The pyrabactin resistance-like (PYL) abscisic acid receptor family has been identified and widely characterized in Arabidopsis. However, PYL families in rice were largely unknown. In the present study, 10 out of 13 PYL orthologs in rice (OsPYL) were isolated and investigated. RESULTS: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that expression of OsPYL genes is tissue-specific and display differential response to ABA treatment, implying their functional diversity. The interaction between 10 OsPYL members and 5 protein phosphatase 2C in rice (OsPP2C) members was investigated in yeast two-hybrid and tobacco transient expression assays, and an overall interaction map was generated, which was suggestive of the diversity and complexity of ABA-sensing signaling in rice. To study the biological function of OsPYLs, two OsPYL genes (OsPYL3 and OsPYL9) were overexpressed in rice. Phenotypic analysis of OsPYL3 and OsPYL9 transgenic rice showed that OsPYLs positively regulated the ABA response during the seed germination. More importantly, the overexpression of OsPYL3 and OsPYL9 substantially improved drought and cold stress tolerance in rice. CONCLUSION: Taken together, we comprehensively uncovered the properties of OsPYLs, which may be good candidates for the improvement of abiotic stress tolerance in rice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12284-015-0061-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-45675722015-09-15 Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice Tian, Xiaojie Wang, Zhenyu Li, Xiufeng Lv, Tianxiao Liu, Huazhao Wang, Lizhi Niu, Hongbin Bu, Qingyun Rice (N Y) Original Article BACKGROUND: Abscisic acid (ABA) plays crucial roles in regulating plant growth and development, especially in responding to abiotic stress. The pyrabactin resistance-like (PYL) abscisic acid receptor family has been identified and widely characterized in Arabidopsis. However, PYL families in rice were largely unknown. In the present study, 10 out of 13 PYL orthologs in rice (OsPYL) were isolated and investigated. RESULTS: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that expression of OsPYL genes is tissue-specific and display differential response to ABA treatment, implying their functional diversity. The interaction between 10 OsPYL members and 5 protein phosphatase 2C in rice (OsPP2C) members was investigated in yeast two-hybrid and tobacco transient expression assays, and an overall interaction map was generated, which was suggestive of the diversity and complexity of ABA-sensing signaling in rice. To study the biological function of OsPYLs, two OsPYL genes (OsPYL3 and OsPYL9) were overexpressed in rice. Phenotypic analysis of OsPYL3 and OsPYL9 transgenic rice showed that OsPYLs positively regulated the ABA response during the seed germination. More importantly, the overexpression of OsPYL3 and OsPYL9 substantially improved drought and cold stress tolerance in rice. CONCLUSION: Taken together, we comprehensively uncovered the properties of OsPYLs, which may be good candidates for the improvement of abiotic stress tolerance in rice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12284-015-0061-6) contains supplementary material, which is available to authorized users. Springer US 2015-09-11 /pmc/articles/PMC4567572/ /pubmed/26362328 http://dx.doi.org/10.1186/s12284-015-0061-6 Text en © Tian et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Tian, Xiaojie
Wang, Zhenyu
Li, Xiufeng
Lv, Tianxiao
Liu, Huazhao
Wang, Lizhi
Niu, Hongbin
Bu, Qingyun
Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice
title Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice
title_full Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice
title_fullStr Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice
title_full_unstemmed Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice
title_short Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice
title_sort characterization and functional analysis of pyrabactin resistance-like abscisic acid receptor family in rice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567572/
https://www.ncbi.nlm.nih.gov/pubmed/26362328
http://dx.doi.org/10.1186/s12284-015-0061-6
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