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Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma

BACKGROUND: It is well-known that the treatment and monitoring methods are limited for advanced stage of endometrial carcinoma. Biological molecules with expression changes during tumor progression become potential therapeutic targets for advanced stage endometrial carcinoma. Annexin A2 (ANXA2) has...

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Autores principales: Deng, Lu, Gao, Yiping, Li, Xiao, Cai, Mingbo, Wang, Huimin, Zhuang, Huiyu, Tan, Mingzi, Liu, Shuice, Hao, Yingying, Lin, Bei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567805/
https://www.ncbi.nlm.nih.gov/pubmed/26362938
http://dx.doi.org/10.1186/s13046-015-0208-8
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author Deng, Lu
Gao, Yiping
Li, Xiao
Cai, Mingbo
Wang, Huimin
Zhuang, Huiyu
Tan, Mingzi
Liu, Shuice
Hao, Yingying
Lin, Bei
author_facet Deng, Lu
Gao, Yiping
Li, Xiao
Cai, Mingbo
Wang, Huimin
Zhuang, Huiyu
Tan, Mingzi
Liu, Shuice
Hao, Yingying
Lin, Bei
author_sort Deng, Lu
collection PubMed
description BACKGROUND: It is well-known that the treatment and monitoring methods are limited for advanced stage of endometrial carcinoma. Biological molecules with expression changes during tumor progression become potential therapeutic targets for advanced stage endometrial carcinoma. Annexin A2 (ANXA2) has been reported to be overexpressed in recurrent endometrial carcinoma, and the expression of human epididymis protein 4 (HE4) is upregulated in endometrial carcinoma. What’s more, ANXA2 and HE4 interacted in ovarian cancer and promoted the malignant biological behavior. We speculated that their interaction may exist in endometrial carcinoma as well. We evaluated the expression and the correlation relationship of ANXA2 and HE4 in endometrial carcinoma. METHODS: The expression of ANXA2 and HE4 protein in 84 endometrial carcinoma, 30 endometrial atypical hyperplasia, and 18 normal endometrial tissue samples were then measured using an immunohistochemical assay in paraffin embedded endometrial tissues. The structural relationship between ANXA2 and HE4 was explored by immunoprecipitation and double immunofluorescent staining. RESULTS: ANXA2 and HE4 co-localized in both endometrial tissues and endometrial carcinoma cells. ANXA2 and HE4 were expressed in 95.2 % and 85.7 % of the the endometrial carcinoma, respectively, which were significantly higher than normal endometrium (55.6 % and 16.7 %, both p < 0.05). The expression of ANXA2 and HE4 was significantly correlated with FIGO stage, degree of differentiation, myometrial invasion, and lymph node metastasis. ANXA2 was an independent risk factor for the prognosis of endometrial carcinoma (p < 0.05, hazard ratio [HR] = 8.004). The expression of ANXA2 and HE4 was positively correlated (Spearman correlation coefficient = 0.228, p < 0.05). HE4 was an independent factor for ANXA2 in multivariate linear regression model (p < 0.05). CONCLUSION: We revealed the co-localization of ANXA2 and HE4 in endometrial carcinoma. Expression levels of ANXA2 and HE4 were closely related to the malignant biological behavior of endometrial carcinoma, and ANXA2 was an independent risk factor for poor prognosis. The expression of ANXA2 and HE4 can affect each other. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13046-015-0208-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-45678052015-09-13 Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma Deng, Lu Gao, Yiping Li, Xiao Cai, Mingbo Wang, Huimin Zhuang, Huiyu Tan, Mingzi Liu, Shuice Hao, Yingying Lin, Bei J Exp Clin Cancer Res Research BACKGROUND: It is well-known that the treatment and monitoring methods are limited for advanced stage of endometrial carcinoma. Biological molecules with expression changes during tumor progression become potential therapeutic targets for advanced stage endometrial carcinoma. Annexin A2 (ANXA2) has been reported to be overexpressed in recurrent endometrial carcinoma, and the expression of human epididymis protein 4 (HE4) is upregulated in endometrial carcinoma. What’s more, ANXA2 and HE4 interacted in ovarian cancer and promoted the malignant biological behavior. We speculated that their interaction may exist in endometrial carcinoma as well. We evaluated the expression and the correlation relationship of ANXA2 and HE4 in endometrial carcinoma. METHODS: The expression of ANXA2 and HE4 protein in 84 endometrial carcinoma, 30 endometrial atypical hyperplasia, and 18 normal endometrial tissue samples were then measured using an immunohistochemical assay in paraffin embedded endometrial tissues. The structural relationship between ANXA2 and HE4 was explored by immunoprecipitation and double immunofluorescent staining. RESULTS: ANXA2 and HE4 co-localized in both endometrial tissues and endometrial carcinoma cells. ANXA2 and HE4 were expressed in 95.2 % and 85.7 % of the the endometrial carcinoma, respectively, which were significantly higher than normal endometrium (55.6 % and 16.7 %, both p < 0.05). The expression of ANXA2 and HE4 was significantly correlated with FIGO stage, degree of differentiation, myometrial invasion, and lymph node metastasis. ANXA2 was an independent risk factor for the prognosis of endometrial carcinoma (p < 0.05, hazard ratio [HR] = 8.004). The expression of ANXA2 and HE4 was positively correlated (Spearman correlation coefficient = 0.228, p < 0.05). HE4 was an independent factor for ANXA2 in multivariate linear regression model (p < 0.05). CONCLUSION: We revealed the co-localization of ANXA2 and HE4 in endometrial carcinoma. Expression levels of ANXA2 and HE4 were closely related to the malignant biological behavior of endometrial carcinoma, and ANXA2 was an independent risk factor for poor prognosis. The expression of ANXA2 and HE4 can affect each other. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13046-015-0208-8) contains supplementary material, which is available to authorized users. BioMed Central 2015-09-11 /pmc/articles/PMC4567805/ /pubmed/26362938 http://dx.doi.org/10.1186/s13046-015-0208-8 Text en © Deng et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Deng, Lu
Gao, Yiping
Li, Xiao
Cai, Mingbo
Wang, Huimin
Zhuang, Huiyu
Tan, Mingzi
Liu, Shuice
Hao, Yingying
Lin, Bei
Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma
title Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma
title_full Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma
title_fullStr Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma
title_full_unstemmed Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma
title_short Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma
title_sort expression and clinical significance of annexin a2 and human epididymis protein 4 in endometrial carcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567805/
https://www.ncbi.nlm.nih.gov/pubmed/26362938
http://dx.doi.org/10.1186/s13046-015-0208-8
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