Cargando…

A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration

This paper presents a tubing-free microfluidic wound healing assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and wound generation. As the first systemic study to q...

Descripción completa

Detalles Bibliográficos
Autores principales: Wei, Yuanchen, Chen, Feng, Zhang, Tao, Chen, Deyong, Jia, Xin, Wang, Junbo, Guo, Wei, Chen, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568460/
https://www.ncbi.nlm.nih.gov/pubmed/26365412
http://dx.doi.org/10.1038/srep14049
_version_ 1782389917405413376
author Wei, Yuanchen
Chen, Feng
Zhang, Tao
Chen, Deyong
Jia, Xin
Wang, Junbo
Guo, Wei
Chen, Jian
author_facet Wei, Yuanchen
Chen, Feng
Zhang, Tao
Chen, Deyong
Jia, Xin
Wang, Junbo
Guo, Wei
Chen, Jian
author_sort Wei, Yuanchen
collection PubMed
description This paper presents a tubing-free microfluidic wound healing assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and wound generation. As the first systemic study to quantify the migration of VSMCs within microfluidic environments, the effects of channel geometries, surface modifications and chemokines on cellular migration were investigated, revealing that 1) height of the micro channels had a significant impact on cell migration; 2) the surface coating of collagen induced more migration of VSMCs than fibronectin coated surfaces and 3) platelet derived growth factor resulted in maximal cell migration compared to tumor necrosis factor alpha and fetal bovine serum. Furthermore, migrations of five types of VSMCs (e.g., the human vascular smooth muscle cell line, two types of primary vascular smooth cells, and VSMCs isolated from two human samples) were quantified, finding that VSMCs from the cell line and human samples demonstrated comparable migration distances, which were significantly lower than the migration distances of two primary cell types. As a platform technology, this wound healing assay may function as a new model to study migration of VSMCs within microfluidic environments.
format Online
Article
Text
id pubmed-4568460
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-45684602015-09-23 A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration Wei, Yuanchen Chen, Feng Zhang, Tao Chen, Deyong Jia, Xin Wang, Junbo Guo, Wei Chen, Jian Sci Rep Article This paper presents a tubing-free microfluidic wound healing assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and wound generation. As the first systemic study to quantify the migration of VSMCs within microfluidic environments, the effects of channel geometries, surface modifications and chemokines on cellular migration were investigated, revealing that 1) height of the micro channels had a significant impact on cell migration; 2) the surface coating of collagen induced more migration of VSMCs than fibronectin coated surfaces and 3) platelet derived growth factor resulted in maximal cell migration compared to tumor necrosis factor alpha and fetal bovine serum. Furthermore, migrations of five types of VSMCs (e.g., the human vascular smooth muscle cell line, two types of primary vascular smooth cells, and VSMCs isolated from two human samples) were quantified, finding that VSMCs from the cell line and human samples demonstrated comparable migration distances, which were significantly lower than the migration distances of two primary cell types. As a platform technology, this wound healing assay may function as a new model to study migration of VSMCs within microfluidic environments. Nature Publishing Group 2015-09-14 /pmc/articles/PMC4568460/ /pubmed/26365412 http://dx.doi.org/10.1038/srep14049 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wei, Yuanchen
Chen, Feng
Zhang, Tao
Chen, Deyong
Jia, Xin
Wang, Junbo
Guo, Wei
Chen, Jian
A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration
title A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration
title_full A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration
title_fullStr A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration
title_full_unstemmed A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration
title_short A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration
title_sort tubing-free microfluidic wound healing assay enabling the quantification of vascular smooth muscle cell migration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568460/
https://www.ncbi.nlm.nih.gov/pubmed/26365412
http://dx.doi.org/10.1038/srep14049
work_keys_str_mv AT weiyuanchen atubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT chenfeng atubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT zhangtao atubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT chendeyong atubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT jiaxin atubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT wangjunbo atubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT guowei atubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT chenjian atubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT weiyuanchen tubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT chenfeng tubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT zhangtao tubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT chendeyong tubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT jiaxin tubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT wangjunbo tubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT guowei tubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration
AT chenjian tubingfreemicrofluidicwoundhealingassayenablingthequantificationofvascularsmoothmusclecellmigration