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Detection of antibiotic resistance in probiotics of dietary supplements

BACKGROUND: Probiotics are live microorganisms that confer nutrition- and health-promoting benefits if consumed in adequate amounts. Concomitant with the demand for natural approaches to maintaining health is an increase in inclusion of probiotics in food and health products. Since probiotic bacteri...

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Autores principales: Wong, Aloysius, Ngu, Davey Yueh Saint, Dan, Lydia Annabel, Ooi, Amanda, Lim, Renee Lay Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568587/
https://www.ncbi.nlm.nih.gov/pubmed/26370532
http://dx.doi.org/10.1186/s12937-015-0084-2
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author Wong, Aloysius
Ngu, Davey Yueh Saint
Dan, Lydia Annabel
Ooi, Amanda
Lim, Renee Lay Hong
author_facet Wong, Aloysius
Ngu, Davey Yueh Saint
Dan, Lydia Annabel
Ooi, Amanda
Lim, Renee Lay Hong
author_sort Wong, Aloysius
collection PubMed
description BACKGROUND: Probiotics are live microorganisms that confer nutrition- and health-promoting benefits if consumed in adequate amounts. Concomitant with the demand for natural approaches to maintaining health is an increase in inclusion of probiotics in food and health products. Since probiotic bacteria act as reservoir for antibiotic resistant determinants, the transfer of these genes to pathogens sharing the same intestinal habitat is thus conceivable considering the fact that dietary supplements contain high amounts of often heterogeneous populations of probiotics. Such events can confer pathogens protection against commonly-used drugs. Despite numerous reports of antibiotic resistant probiotics in food and biological sources, the antibiogram of probiotics from dietary supplements remained elusive. FINDINGS: Here, we screened five commercially available dietary supplements for resistance towards antibiotics of different classes. Probiotics of all batches of products were resistant towards vancomycin while batch-dependent resistance towards streptomycin, aztreonam, gentamycin and/or ciprofloxacin antibiotics was detected for probiotics of brands Bi and Bn, Bg, and L. Isolates of brand Cn was also resistant towards gentamycin, streptomycin and ciprofloxacin antibiotics. Additionally, we also report a discrepancy between the enumerated viable bacteria amounts and the claims of the manufacturers. CONCLUSIONS: This short report has highlighted the present of antibiotic resistance in probiotic bacteria from dietary supplements and therefore serves as a platform for further screenings and for in-depth characterization of the resistant determinants and the molecular machinery that confers the resistance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12937-015-0084-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-45685872015-09-15 Detection of antibiotic resistance in probiotics of dietary supplements Wong, Aloysius Ngu, Davey Yueh Saint Dan, Lydia Annabel Ooi, Amanda Lim, Renee Lay Hong Nutr J Short Report BACKGROUND: Probiotics are live microorganisms that confer nutrition- and health-promoting benefits if consumed in adequate amounts. Concomitant with the demand for natural approaches to maintaining health is an increase in inclusion of probiotics in food and health products. Since probiotic bacteria act as reservoir for antibiotic resistant determinants, the transfer of these genes to pathogens sharing the same intestinal habitat is thus conceivable considering the fact that dietary supplements contain high amounts of often heterogeneous populations of probiotics. Such events can confer pathogens protection against commonly-used drugs. Despite numerous reports of antibiotic resistant probiotics in food and biological sources, the antibiogram of probiotics from dietary supplements remained elusive. FINDINGS: Here, we screened five commercially available dietary supplements for resistance towards antibiotics of different classes. Probiotics of all batches of products were resistant towards vancomycin while batch-dependent resistance towards streptomycin, aztreonam, gentamycin and/or ciprofloxacin antibiotics was detected for probiotics of brands Bi and Bn, Bg, and L. Isolates of brand Cn was also resistant towards gentamycin, streptomycin and ciprofloxacin antibiotics. Additionally, we also report a discrepancy between the enumerated viable bacteria amounts and the claims of the manufacturers. CONCLUSIONS: This short report has highlighted the present of antibiotic resistance in probiotic bacteria from dietary supplements and therefore serves as a platform for further screenings and for in-depth characterization of the resistant determinants and the molecular machinery that confers the resistance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12937-015-0084-2) contains supplementary material, which is available to authorized users. BioMed Central 2015-09-14 /pmc/articles/PMC4568587/ /pubmed/26370532 http://dx.doi.org/10.1186/s12937-015-0084-2 Text en © Wong et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Wong, Aloysius
Ngu, Davey Yueh Saint
Dan, Lydia Annabel
Ooi, Amanda
Lim, Renee Lay Hong
Detection of antibiotic resistance in probiotics of dietary supplements
title Detection of antibiotic resistance in probiotics of dietary supplements
title_full Detection of antibiotic resistance in probiotics of dietary supplements
title_fullStr Detection of antibiotic resistance in probiotics of dietary supplements
title_full_unstemmed Detection of antibiotic resistance in probiotics of dietary supplements
title_short Detection of antibiotic resistance in probiotics of dietary supplements
title_sort detection of antibiotic resistance in probiotics of dietary supplements
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568587/
https://www.ncbi.nlm.nih.gov/pubmed/26370532
http://dx.doi.org/10.1186/s12937-015-0084-2
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