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Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568857/ https://www.ncbi.nlm.nih.gov/pubmed/26413048 http://dx.doi.org/10.1590/S1517-838246320131289 |
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author | Li, Jiang Hu, Qiushi Li, Yuquan Xu, Yuan |
author_facet | Li, Jiang Hu, Qiushi Li, Yuquan Xu, Yuan |
author_sort | Li, Jiang |
collection | PubMed |
description | An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg(2+), Mn(2+), K(+); Ca(2+), Na(+), Ba(2+), Zn(2+), Cu(2+), Co(2+), Fe(2+), Sr(2+) and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries. |
format | Online Article Text |
id | pubmed-4568857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-45688572015-09-25 Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain Li, Jiang Hu, Qiushi Li, Yuquan Xu, Yuan Braz J Microbiol Environmental Microbiology An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg(2+), Mn(2+), K(+); Ca(2+), Na(+), Ba(2+), Zn(2+), Cu(2+), Co(2+), Fe(2+), Sr(2+) and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries. Sociedade Brasileira de Microbiologia 2015-07-01 /pmc/articles/PMC4568857/ /pubmed/26413048 http://dx.doi.org/10.1590/S1517-838246320131289 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Environmental Microbiology Li, Jiang Hu, Qiushi Li, Yuquan Xu, Yuan Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain |
title | Purification and characterization of cold-adapted beta-agarase from an
Antarctic psychrophilic strain |
title_full | Purification and characterization of cold-adapted beta-agarase from an
Antarctic psychrophilic strain |
title_fullStr | Purification and characterization of cold-adapted beta-agarase from an
Antarctic psychrophilic strain |
title_full_unstemmed | Purification and characterization of cold-adapted beta-agarase from an
Antarctic psychrophilic strain |
title_short | Purification and characterization of cold-adapted beta-agarase from an
Antarctic psychrophilic strain |
title_sort | purification and characterization of cold-adapted beta-agarase from an
antarctic psychrophilic strain |
topic | Environmental Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568857/ https://www.ncbi.nlm.nih.gov/pubmed/26413048 http://dx.doi.org/10.1590/S1517-838246320131289 |
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