Cargando…

Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain

An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Jiang, Hu, Qiushi, Li, Yuquan, Xu, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568857/
https://www.ncbi.nlm.nih.gov/pubmed/26413048
http://dx.doi.org/10.1590/S1517-838246320131289
_version_ 1782389955167780864
author Li, Jiang
Hu, Qiushi
Li, Yuquan
Xu, Yuan
author_facet Li, Jiang
Hu, Qiushi
Li, Yuquan
Xu, Yuan
author_sort Li, Jiang
collection PubMed
description An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg(2+), Mn(2+), K(+); Ca(2+), Na(+), Ba(2+), Zn(2+), Cu(2+), Co(2+), Fe(2+), Sr(2+) and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.
format Online
Article
Text
id pubmed-4568857
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-45688572015-09-25 Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain Li, Jiang Hu, Qiushi Li, Yuquan Xu, Yuan Braz J Microbiol Environmental Microbiology An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg(2+), Mn(2+), K(+); Ca(2+), Na(+), Ba(2+), Zn(2+), Cu(2+), Co(2+), Fe(2+), Sr(2+) and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries. Sociedade Brasileira de Microbiologia 2015-07-01 /pmc/articles/PMC4568857/ /pubmed/26413048 http://dx.doi.org/10.1590/S1517-838246320131289 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Environmental Microbiology
Li, Jiang
Hu, Qiushi
Li, Yuquan
Xu, Yuan
Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
title Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
title_full Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
title_fullStr Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
title_full_unstemmed Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
title_short Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
title_sort purification and characterization of cold-adapted beta-agarase from an antarctic psychrophilic strain
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568857/
https://www.ncbi.nlm.nih.gov/pubmed/26413048
http://dx.doi.org/10.1590/S1517-838246320131289
work_keys_str_mv AT lijiang purificationandcharacterizationofcoldadaptedbetaagarasefromanantarcticpsychrophilicstrain
AT huqiushi purificationandcharacterizationofcoldadaptedbetaagarasefromanantarcticpsychrophilicstrain
AT liyuquan purificationandcharacterizationofcoldadaptedbetaagarasefromanantarcticpsychrophilicstrain
AT xuyuan purificationandcharacterizationofcoldadaptedbetaagarasefromanantarcticpsychrophilicstrain