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Symbiotic potential and survival of native rhizobia kept on different carriers

Native rhizobia are ideal for use as commercial legume inoculants. The characteristics of the carrier used to store the inoculants are important for the survival and symbiotic potential of the rhizobia. The objective of this study was to investigate the effects of peat (PEAT), perlite sugarcane baga...

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Autores principales: Ruíz-Valdiviezo, Víctor Manuel, Canseco, Lucía María Cristina Ventura, Suárez, Luis Antonio Castillo, Gutiérrez-Miceli, Federico Antonio, Dendooven, Luc, Rincón-Rosales, Reiner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568871/
https://www.ncbi.nlm.nih.gov/pubmed/26413054
http://dx.doi.org/10.1590/S1517-838246320140541
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author Ruíz-Valdiviezo, Víctor Manuel
Canseco, Lucía María Cristina Ventura
Suárez, Luis Antonio Castillo
Gutiérrez-Miceli, Federico Antonio
Dendooven, Luc
Rincón-Rosales, Reiner
author_facet Ruíz-Valdiviezo, Víctor Manuel
Canseco, Lucía María Cristina Ventura
Suárez, Luis Antonio Castillo
Gutiérrez-Miceli, Federico Antonio
Dendooven, Luc
Rincón-Rosales, Reiner
author_sort Ruíz-Valdiviezo, Víctor Manuel
collection PubMed
description Native rhizobia are ideal for use as commercial legume inoculants. The characteristics of the carrier used to store the inoculants are important for the survival and symbiotic potential of the rhizobia. The objective of this study was to investigate the effects of peat (PEAT), perlite sugarcane bagasse (PSB), carboxymethyl cellulose plus starch (CMCS), and yeast extract mannitol supplemented with mannitol (YEMM) on the survival, nodulation potential and N(2) fixation capacity of the native strains Sinorhizobium mexicanum ITTG R7(T) and Rhizobium calliandrae LBP2-1(T) and of the reference strain Rhizobium etli CFN42(T). A factorial design (4 × 3) with four repetitions was used to determine the symbiotic potential of the rhizobial strains. The survival of the strains was higher for PEAT (46% for strain LBP2-1(T), 167% for strain CFN42(T) and 219% for strain ITTG R7(T)) than for the other carriers after 240 days, except for CFN42(T) kept on CMCS (225%). All the strains kept on the different carriers effectively nodulated common bean, with the lowest number of nodules found (5 nodules) when CFN42(T) was kept on CMCS and with the highest number of nodules found (28 nodules) when ITTG R7(T) was kept on PSB. The nitrogenase activity was the highest for ITTG R7(T) kept on PEAT (4911 μmol C(2)H(4) per fresh weight nodule h(−1)); however, no activity was found when the strains were kept on YEMM. Thus, the survival and symbiotic potential of the rhizobia depended on the carrier used to store them.
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spelling pubmed-45688712015-09-25 Symbiotic potential and survival of native rhizobia kept on different carriers Ruíz-Valdiviezo, Víctor Manuel Canseco, Lucía María Cristina Ventura Suárez, Luis Antonio Castillo Gutiérrez-Miceli, Federico Antonio Dendooven, Luc Rincón-Rosales, Reiner Braz J Microbiol Environmental Microbiology Native rhizobia are ideal for use as commercial legume inoculants. The characteristics of the carrier used to store the inoculants are important for the survival and symbiotic potential of the rhizobia. The objective of this study was to investigate the effects of peat (PEAT), perlite sugarcane bagasse (PSB), carboxymethyl cellulose plus starch (CMCS), and yeast extract mannitol supplemented with mannitol (YEMM) on the survival, nodulation potential and N(2) fixation capacity of the native strains Sinorhizobium mexicanum ITTG R7(T) and Rhizobium calliandrae LBP2-1(T) and of the reference strain Rhizobium etli CFN42(T). A factorial design (4 × 3) with four repetitions was used to determine the symbiotic potential of the rhizobial strains. The survival of the strains was higher for PEAT (46% for strain LBP2-1(T), 167% for strain CFN42(T) and 219% for strain ITTG R7(T)) than for the other carriers after 240 days, except for CFN42(T) kept on CMCS (225%). All the strains kept on the different carriers effectively nodulated common bean, with the lowest number of nodules found (5 nodules) when CFN42(T) was kept on CMCS and with the highest number of nodules found (28 nodules) when ITTG R7(T) was kept on PSB. The nitrogenase activity was the highest for ITTG R7(T) kept on PEAT (4911 μmol C(2)H(4) per fresh weight nodule h(−1)); however, no activity was found when the strains were kept on YEMM. Thus, the survival and symbiotic potential of the rhizobia depended on the carrier used to store them. Sociedade Brasileira de Microbiologia 2015-07-01 /pmc/articles/PMC4568871/ /pubmed/26413054 http://dx.doi.org/10.1590/S1517-838246320140541 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Environmental Microbiology
Ruíz-Valdiviezo, Víctor Manuel
Canseco, Lucía María Cristina Ventura
Suárez, Luis Antonio Castillo
Gutiérrez-Miceli, Federico Antonio
Dendooven, Luc
Rincón-Rosales, Reiner
Symbiotic potential and survival of native rhizobia kept on different carriers
title Symbiotic potential and survival of native rhizobia kept on different carriers
title_full Symbiotic potential and survival of native rhizobia kept on different carriers
title_fullStr Symbiotic potential and survival of native rhizobia kept on different carriers
title_full_unstemmed Symbiotic potential and survival of native rhizobia kept on different carriers
title_short Symbiotic potential and survival of native rhizobia kept on different carriers
title_sort symbiotic potential and survival of native rhizobia kept on different carriers
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568871/
https://www.ncbi.nlm.nih.gov/pubmed/26413054
http://dx.doi.org/10.1590/S1517-838246320140541
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