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Symbiotic potential and survival of native rhizobia kept on different carriers
Native rhizobia are ideal for use as commercial legume inoculants. The characteristics of the carrier used to store the inoculants are important for the survival and symbiotic potential of the rhizobia. The objective of this study was to investigate the effects of peat (PEAT), perlite sugarcane baga...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568871/ https://www.ncbi.nlm.nih.gov/pubmed/26413054 http://dx.doi.org/10.1590/S1517-838246320140541 |
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author | Ruíz-Valdiviezo, Víctor Manuel Canseco, Lucía María Cristina Ventura Suárez, Luis Antonio Castillo Gutiérrez-Miceli, Federico Antonio Dendooven, Luc Rincón-Rosales, Reiner |
author_facet | Ruíz-Valdiviezo, Víctor Manuel Canseco, Lucía María Cristina Ventura Suárez, Luis Antonio Castillo Gutiérrez-Miceli, Federico Antonio Dendooven, Luc Rincón-Rosales, Reiner |
author_sort | Ruíz-Valdiviezo, Víctor Manuel |
collection | PubMed |
description | Native rhizobia are ideal for use as commercial legume inoculants. The characteristics of the carrier used to store the inoculants are important for the survival and symbiotic potential of the rhizobia. The objective of this study was to investigate the effects of peat (PEAT), perlite sugarcane bagasse (PSB), carboxymethyl cellulose plus starch (CMCS), and yeast extract mannitol supplemented with mannitol (YEMM) on the survival, nodulation potential and N(2) fixation capacity of the native strains Sinorhizobium mexicanum ITTG R7(T) and Rhizobium calliandrae LBP2-1(T) and of the reference strain Rhizobium etli CFN42(T). A factorial design (4 × 3) with four repetitions was used to determine the symbiotic potential of the rhizobial strains. The survival of the strains was higher for PEAT (46% for strain LBP2-1(T), 167% for strain CFN42(T) and 219% for strain ITTG R7(T)) than for the other carriers after 240 days, except for CFN42(T) kept on CMCS (225%). All the strains kept on the different carriers effectively nodulated common bean, with the lowest number of nodules found (5 nodules) when CFN42(T) was kept on CMCS and with the highest number of nodules found (28 nodules) when ITTG R7(T) was kept on PSB. The nitrogenase activity was the highest for ITTG R7(T) kept on PEAT (4911 μmol C(2)H(4) per fresh weight nodule h(−1)); however, no activity was found when the strains were kept on YEMM. Thus, the survival and symbiotic potential of the rhizobia depended on the carrier used to store them. |
format | Online Article Text |
id | pubmed-4568871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-45688712015-09-25 Symbiotic potential and survival of native rhizobia kept on different carriers Ruíz-Valdiviezo, Víctor Manuel Canseco, Lucía María Cristina Ventura Suárez, Luis Antonio Castillo Gutiérrez-Miceli, Federico Antonio Dendooven, Luc Rincón-Rosales, Reiner Braz J Microbiol Environmental Microbiology Native rhizobia are ideal for use as commercial legume inoculants. The characteristics of the carrier used to store the inoculants are important for the survival and symbiotic potential of the rhizobia. The objective of this study was to investigate the effects of peat (PEAT), perlite sugarcane bagasse (PSB), carboxymethyl cellulose plus starch (CMCS), and yeast extract mannitol supplemented with mannitol (YEMM) on the survival, nodulation potential and N(2) fixation capacity of the native strains Sinorhizobium mexicanum ITTG R7(T) and Rhizobium calliandrae LBP2-1(T) and of the reference strain Rhizobium etli CFN42(T). A factorial design (4 × 3) with four repetitions was used to determine the symbiotic potential of the rhizobial strains. The survival of the strains was higher for PEAT (46% for strain LBP2-1(T), 167% for strain CFN42(T) and 219% for strain ITTG R7(T)) than for the other carriers after 240 days, except for CFN42(T) kept on CMCS (225%). All the strains kept on the different carriers effectively nodulated common bean, with the lowest number of nodules found (5 nodules) when CFN42(T) was kept on CMCS and with the highest number of nodules found (28 nodules) when ITTG R7(T) was kept on PSB. The nitrogenase activity was the highest for ITTG R7(T) kept on PEAT (4911 μmol C(2)H(4) per fresh weight nodule h(−1)); however, no activity was found when the strains were kept on YEMM. Thus, the survival and symbiotic potential of the rhizobia depended on the carrier used to store them. Sociedade Brasileira de Microbiologia 2015-07-01 /pmc/articles/PMC4568871/ /pubmed/26413054 http://dx.doi.org/10.1590/S1517-838246320140541 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Environmental Microbiology Ruíz-Valdiviezo, Víctor Manuel Canseco, Lucía María Cristina Ventura Suárez, Luis Antonio Castillo Gutiérrez-Miceli, Federico Antonio Dendooven, Luc Rincón-Rosales, Reiner Symbiotic potential and survival of native rhizobia kept on different carriers |
title | Symbiotic potential and survival of native rhizobia kept on different
carriers |
title_full | Symbiotic potential and survival of native rhizobia kept on different
carriers |
title_fullStr | Symbiotic potential and survival of native rhizobia kept on different
carriers |
title_full_unstemmed | Symbiotic potential and survival of native rhizobia kept on different
carriers |
title_short | Symbiotic potential and survival of native rhizobia kept on different
carriers |
title_sort | symbiotic potential and survival of native rhizobia kept on different
carriers |
topic | Environmental Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568871/ https://www.ncbi.nlm.nih.gov/pubmed/26413054 http://dx.doi.org/10.1590/S1517-838246320140541 |
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