Characterization of Francisella species isolated from the cooling water of an air conditioning system

Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition, these bacteria grow on cysteine-supplem...

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Autores principales: Gu, Quan, Li, Xunde, Qu, Pinghua, Hou, Shuiping, Li, Juntao, Atwill, Edward R., Chen, Shouyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Genetics and Molecular Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568874/
https://www.ncbi.nlm.nih.gov/pubmed/26413079
http://dx.doi.org/10.1590/S1517-838246320140465
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author Gu, Quan
Li, Xunde
Qu, Pinghua
Hou, Shuiping
Li, Juntao
Atwill, Edward R.
Chen, Shouyi
author_facet Gu, Quan
Li, Xunde
Qu, Pinghua
Hou, Shuiping
Li, Juntao
Atwill, Edward R.
Chen, Shouyi
author_sort Gu, Quan
collection PubMed
description Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition, these bacteria grow on cysteine-supplemented media at 20 °C to 40 °C with an optimal growth temperature of 30 °C. Analysis of 16S rRNA gene sequences revealed that these strains belong to the genus Francisella. Biochemical tests and phylogenetic and BLAST analyses of 16S rRNA, rpoB and sdhA genes indicated that one strain was very similar to Francisella philomiragia and that the other strains were identical or highly similar to the Francisella guangzhouensis sp. nov. strain 08HL01032 we previously described. Biochemical and molecular characteristics of these strains demonstrated that multiple Francisella species exist in air conditioning systems.
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spelling pubmed-45688742015-09-25 Characterization of Francisella species isolated from the cooling water of an air conditioning system Gu, Quan Li, Xunde Qu, Pinghua Hou, Shuiping Li, Juntao Atwill, Edward R. Chen, Shouyi Braz J Microbiol Genetics and Molecular Microbiology Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition, these bacteria grow on cysteine-supplemented media at 20 °C to 40 °C with an optimal growth temperature of 30 °C. Analysis of 16S rRNA gene sequences revealed that these strains belong to the genus Francisella. Biochemical tests and phylogenetic and BLAST analyses of 16S rRNA, rpoB and sdhA genes indicated that one strain was very similar to Francisella philomiragia and that the other strains were identical or highly similar to the Francisella guangzhouensis sp. nov. strain 08HL01032 we previously described. Biochemical and molecular characteristics of these strains demonstrated that multiple Francisella species exist in air conditioning systems. Sociedade Brasileira de Microbiologia 2015-07-01 /pmc/articles/PMC4568874/ /pubmed/26413079 http://dx.doi.org/10.1590/S1517-838246320140465 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Genetics and Molecular Microbiology
Gu, Quan
Li, Xunde
Qu, Pinghua
Hou, Shuiping
Li, Juntao
Atwill, Edward R.
Chen, Shouyi
Characterization of Francisella species isolated from the cooling water of an air conditioning system
title Characterization of Francisella species isolated from the cooling water of an air conditioning system
title_full Characterization of Francisella species isolated from the cooling water of an air conditioning system
title_fullStr Characterization of Francisella species isolated from the cooling water of an air conditioning system
title_full_unstemmed Characterization of Francisella species isolated from the cooling water of an air conditioning system
title_short Characterization of Francisella species isolated from the cooling water of an air conditioning system
title_sort characterization of francisella species isolated from the cooling water of an air conditioning system
topic Genetics and Molecular Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568874/
https://www.ncbi.nlm.nih.gov/pubmed/26413079
http://dx.doi.org/10.1590/S1517-838246320140465
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