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Keratinolytic abilities of Micrococcus luteus from poultry waste

Keratinolytic microorganisms have become the subject of scientific interest due to their ability to biosynthesize specific keratinases and their prospective application in keratinic waste management. Among several bacterial classes, actinobacteria remain one of the most important sources of keratin-...

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Autores principales: Laba, Wojciech, Choinska, Anna, Rodziewicz, Anna, Piegza, Michal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568876/
https://www.ncbi.nlm.nih.gov/pubmed/26413049
http://dx.doi.org/10.1590/S1517-838246320140098
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author Laba, Wojciech
Choinska, Anna
Rodziewicz, Anna
Piegza, Michal
author_facet Laba, Wojciech
Choinska, Anna
Rodziewicz, Anna
Piegza, Michal
author_sort Laba, Wojciech
collection PubMed
description Keratinolytic microorganisms have become the subject of scientific interest due to their ability to biosynthesize specific keratinases and their prospective application in keratinic waste management. Among several bacterial classes, actinobacteria remain one of the most important sources of keratin-degrading strains, however members of the Micrococcaceae family are rarely scrutinized in regard to their applicatory keratinolytic potential. The tested Micrococcus sp. B1pz isolate from poultry feather waste was identified as M. luteus. The strain, grown in the medium with 1–2% chicken feathers and a yeast extract supplement, produced keratinases of 32 KU and lower level of proteases, 6 PU. It was capable to effectively decompose feathers or “soft” keratin of stratum corneum, in contrast to other “hard” hair-type keratins. The produced keratinolytic enzymes were mainly a combination of alkaline serine or thiol proteases, active at the optimum pH 9.4, 55 °C. Four main protease fractions of 62, 185, 139 and 229 kDa were identified in the crude culture fluid. The research on the auxiliary role of reducing factors revealed that reducing sulfur compounds could be applied in keratinolysis enhancement during enzymatic digestion of keratin, rather than in culture conditions. The presented M. luteus isolate exhibits a significant keratinolytic potential, which determines its feasible applicatory capacity towards biodegradation of poultry by-products or formulation of keratin-based feed components.
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spelling pubmed-45688762015-09-25 Keratinolytic abilities of Micrococcus luteus from poultry waste Laba, Wojciech Choinska, Anna Rodziewicz, Anna Piegza, Michal Braz J Microbiol Environmental Microbiology Keratinolytic microorganisms have become the subject of scientific interest due to their ability to biosynthesize specific keratinases and their prospective application in keratinic waste management. Among several bacterial classes, actinobacteria remain one of the most important sources of keratin-degrading strains, however members of the Micrococcaceae family are rarely scrutinized in regard to their applicatory keratinolytic potential. The tested Micrococcus sp. B1pz isolate from poultry feather waste was identified as M. luteus. The strain, grown in the medium with 1–2% chicken feathers and a yeast extract supplement, produced keratinases of 32 KU and lower level of proteases, 6 PU. It was capable to effectively decompose feathers or “soft” keratin of stratum corneum, in contrast to other “hard” hair-type keratins. The produced keratinolytic enzymes were mainly a combination of alkaline serine or thiol proteases, active at the optimum pH 9.4, 55 °C. Four main protease fractions of 62, 185, 139 and 229 kDa were identified in the crude culture fluid. The research on the auxiliary role of reducing factors revealed that reducing sulfur compounds could be applied in keratinolysis enhancement during enzymatic digestion of keratin, rather than in culture conditions. The presented M. luteus isolate exhibits a significant keratinolytic potential, which determines its feasible applicatory capacity towards biodegradation of poultry by-products or formulation of keratin-based feed components. Sociedade Brasileira de Microbiologia 2015-07-01 /pmc/articles/PMC4568876/ /pubmed/26413049 http://dx.doi.org/10.1590/S1517-838246320140098 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Environmental Microbiology
Laba, Wojciech
Choinska, Anna
Rodziewicz, Anna
Piegza, Michal
Keratinolytic abilities of Micrococcus luteus from poultry waste
title Keratinolytic abilities of Micrococcus luteus from poultry waste
title_full Keratinolytic abilities of Micrococcus luteus from poultry waste
title_fullStr Keratinolytic abilities of Micrococcus luteus from poultry waste
title_full_unstemmed Keratinolytic abilities of Micrococcus luteus from poultry waste
title_short Keratinolytic abilities of Micrococcus luteus from poultry waste
title_sort keratinolytic abilities of micrococcus luteus from poultry waste
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568876/
https://www.ncbi.nlm.nih.gov/pubmed/26413049
http://dx.doi.org/10.1590/S1517-838246320140098
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