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In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions

The goal of this research was to study the long-term impact of medical interventions on circulating tumor cell (CTC) dynamics. We have explored whether tumor compression, punch biopsy or tumor resection cause dissemination of CTCs into peripheral blood circulation using in vivo fluorescent flow cyto...

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Autores principales: Juratli, Mazen A., Siegel, Eric R., Nedosekin, Dmitry A., Sarimollaoglu, Mustafa, Jamshidi-Parsian, Azemat, Cai, Chengzhong, Menyaev, Yulian A., Suen, James Y., Galanzha, Ekaterina I., Zharov, Vladimir P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569172/
https://www.ncbi.nlm.nih.gov/pubmed/26367280
http://dx.doi.org/10.1371/journal.pone.0137613
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author Juratli, Mazen A.
Siegel, Eric R.
Nedosekin, Dmitry A.
Sarimollaoglu, Mustafa
Jamshidi-Parsian, Azemat
Cai, Chengzhong
Menyaev, Yulian A.
Suen, James Y.
Galanzha, Ekaterina I.
Zharov, Vladimir P.
author_facet Juratli, Mazen A.
Siegel, Eric R.
Nedosekin, Dmitry A.
Sarimollaoglu, Mustafa
Jamshidi-Parsian, Azemat
Cai, Chengzhong
Menyaev, Yulian A.
Suen, James Y.
Galanzha, Ekaterina I.
Zharov, Vladimir P.
author_sort Juratli, Mazen A.
collection PubMed
description The goal of this research was to study the long-term impact of medical interventions on circulating tumor cell (CTC) dynamics. We have explored whether tumor compression, punch biopsy or tumor resection cause dissemination of CTCs into peripheral blood circulation using in vivo fluorescent flow cytometry and breast cancer-bearing mouse model inoculated with MDA-MB-231-Luc2-GFP cells in the mammary gland. Two weeks after tumor inoculation, three groups of mice were the subject of the following interventions: (1) tumor compression for 15 minutes using 400 g weight to approximate the pressure during mammography; (2) punch biopsy; or (3) surgery. The CTC dynamics were determined before, during and six weeks after these interventions. An additional group of tumor-bearing mice was used as control and did not receive an intervention. The CTC dynamics in all mice were monitored weekly for eight weeks after tumor inoculation. We determined that tumor compression did not significantly affect CTC dynamics, either during the procedure itself (P = 0.28), or during the 6-week follow-up. In the punch biopsy group, we observed a significant increase in CTC immediately after the biopsy (P = 0.02), and the rate stayed elevated up to six weeks after the procedure in comparison to the tumor control group. The CTCs in the group of mice that received a tumor resection disappeared immediately after the surgery (P = 0.03). However, CTC recurrence in small numbers was detected during six weeks after the surgery. In the future, to prevent these side effects of medical interventions, the defined dynamics of intervention-induced CTCs may be used as a basis for initiation of aggressive anti-CTC therapy at time-points of increasing CTC number.
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spelling pubmed-45691722015-09-18 In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions Juratli, Mazen A. Siegel, Eric R. Nedosekin, Dmitry A. Sarimollaoglu, Mustafa Jamshidi-Parsian, Azemat Cai, Chengzhong Menyaev, Yulian A. Suen, James Y. Galanzha, Ekaterina I. Zharov, Vladimir P. PLoS One Research Article The goal of this research was to study the long-term impact of medical interventions on circulating tumor cell (CTC) dynamics. We have explored whether tumor compression, punch biopsy or tumor resection cause dissemination of CTCs into peripheral blood circulation using in vivo fluorescent flow cytometry and breast cancer-bearing mouse model inoculated with MDA-MB-231-Luc2-GFP cells in the mammary gland. Two weeks after tumor inoculation, three groups of mice were the subject of the following interventions: (1) tumor compression for 15 minutes using 400 g weight to approximate the pressure during mammography; (2) punch biopsy; or (3) surgery. The CTC dynamics were determined before, during and six weeks after these interventions. An additional group of tumor-bearing mice was used as control and did not receive an intervention. The CTC dynamics in all mice were monitored weekly for eight weeks after tumor inoculation. We determined that tumor compression did not significantly affect CTC dynamics, either during the procedure itself (P = 0.28), or during the 6-week follow-up. In the punch biopsy group, we observed a significant increase in CTC immediately after the biopsy (P = 0.02), and the rate stayed elevated up to six weeks after the procedure in comparison to the tumor control group. The CTCs in the group of mice that received a tumor resection disappeared immediately after the surgery (P = 0.03). However, CTC recurrence in small numbers was detected during six weeks after the surgery. In the future, to prevent these side effects of medical interventions, the defined dynamics of intervention-induced CTCs may be used as a basis for initiation of aggressive anti-CTC therapy at time-points of increasing CTC number. Public Library of Science 2015-09-14 /pmc/articles/PMC4569172/ /pubmed/26367280 http://dx.doi.org/10.1371/journal.pone.0137613 Text en © 2015 Juratli et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Juratli, Mazen A.
Siegel, Eric R.
Nedosekin, Dmitry A.
Sarimollaoglu, Mustafa
Jamshidi-Parsian, Azemat
Cai, Chengzhong
Menyaev, Yulian A.
Suen, James Y.
Galanzha, Ekaterina I.
Zharov, Vladimir P.
In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions
title In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions
title_full In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions
title_fullStr In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions
title_full_unstemmed In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions
title_short In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions
title_sort in vivo long-term monitoring of circulating tumor cells fluctuation during medical interventions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569172/
https://www.ncbi.nlm.nih.gov/pubmed/26367280
http://dx.doi.org/10.1371/journal.pone.0137613
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