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Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis
Hypersensitivity pneumonitis (HP) is an immune mediated lung disease induced by the repeated inhalation of a wide variety of antigens. Bird-related hypersensitivity pneumonitis (BRHP) is one of the most common forms of HP in human and results from the inhalation of avian antigens. The findings of a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569356/ https://www.ncbi.nlm.nih.gov/pubmed/26367130 http://dx.doi.org/10.1371/journal.pone.0137978 |
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author | Ishizuka, Masahiro Miyazaki, Yasunari Masuo, Masahiro Suhara, Kozo Tateishi, Tomoya Yasui, Makito Inase, Naohiko |
author_facet | Ishizuka, Masahiro Miyazaki, Yasunari Masuo, Masahiro Suhara, Kozo Tateishi, Tomoya Yasui, Makito Inase, Naohiko |
author_sort | Ishizuka, Masahiro |
collection | PubMed |
description | Hypersensitivity pneumonitis (HP) is an immune mediated lung disease induced by the repeated inhalation of a wide variety of antigens. Bird-related hypersensitivity pneumonitis (BRHP) is one of the most common forms of HP in human and results from the inhalation of avian antigens. The findings of a recent clinical analysis suggest that in addition to Th1 factors, the levels of interleukin(IL)-17 and IL-17-associated transcripts are increased in the setting of HP, and that both IL-17A and neutrophils are crucial for the development of pulmonary inflammation in murine models of HP. Our objectives were to investigate the roles of IL-17A and neutrophils in granuloma-forming inflammation in an acute HP model. We developed a mouse model of acute BRHP using pigeon dropping extract. We evaluated the process of granuloma formation and the roles of both IL-17A and neutrophils in a model. We found that the neutralization of IL-17A by the antibody attenuated granuloma formation and the recruitment of neutrophils, and also decreased the expression level of chemokine(C-X-C motif) ligand 5 (CXCL5) in the acute HP model. We confirmed that most of the neutrophils in the acute HP model exhibited immunoreactivity to the anti-IL-17 antibody. We have identified the central roles of both IL-17A and neutrophils in the pathogenesis of granuloma formation in acute HP. We have also assumed that neutrophils are an important source of IL-17A in an acute HP model, and that the IL-17A-CXCL5 pathway may be responsible for the recruitment of neutrophils. |
format | Online Article Text |
id | pubmed-4569356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45693562015-09-18 Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis Ishizuka, Masahiro Miyazaki, Yasunari Masuo, Masahiro Suhara, Kozo Tateishi, Tomoya Yasui, Makito Inase, Naohiko PLoS One Research Article Hypersensitivity pneumonitis (HP) is an immune mediated lung disease induced by the repeated inhalation of a wide variety of antigens. Bird-related hypersensitivity pneumonitis (BRHP) is one of the most common forms of HP in human and results from the inhalation of avian antigens. The findings of a recent clinical analysis suggest that in addition to Th1 factors, the levels of interleukin(IL)-17 and IL-17-associated transcripts are increased in the setting of HP, and that both IL-17A and neutrophils are crucial for the development of pulmonary inflammation in murine models of HP. Our objectives were to investigate the roles of IL-17A and neutrophils in granuloma-forming inflammation in an acute HP model. We developed a mouse model of acute BRHP using pigeon dropping extract. We evaluated the process of granuloma formation and the roles of both IL-17A and neutrophils in a model. We found that the neutralization of IL-17A by the antibody attenuated granuloma formation and the recruitment of neutrophils, and also decreased the expression level of chemokine(C-X-C motif) ligand 5 (CXCL5) in the acute HP model. We confirmed that most of the neutrophils in the acute HP model exhibited immunoreactivity to the anti-IL-17 antibody. We have identified the central roles of both IL-17A and neutrophils in the pathogenesis of granuloma formation in acute HP. We have also assumed that neutrophils are an important source of IL-17A in an acute HP model, and that the IL-17A-CXCL5 pathway may be responsible for the recruitment of neutrophils. Public Library of Science 2015-09-14 /pmc/articles/PMC4569356/ /pubmed/26367130 http://dx.doi.org/10.1371/journal.pone.0137978 Text en © 2015 Ishizuka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ishizuka, Masahiro Miyazaki, Yasunari Masuo, Masahiro Suhara, Kozo Tateishi, Tomoya Yasui, Makito Inase, Naohiko Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis |
title | Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis |
title_full | Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis |
title_fullStr | Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis |
title_full_unstemmed | Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis |
title_short | Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis |
title_sort | interleukin-17a and neutrophils in a murine model of bird-related hypersensitivity pneumonitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569356/ https://www.ncbi.nlm.nih.gov/pubmed/26367130 http://dx.doi.org/10.1371/journal.pone.0137978 |
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