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Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis

Hypersensitivity pneumonitis (HP) is an immune mediated lung disease induced by the repeated inhalation of a wide variety of antigens. Bird-related hypersensitivity pneumonitis (BRHP) is one of the most common forms of HP in human and results from the inhalation of avian antigens. The findings of a...

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Autores principales: Ishizuka, Masahiro, Miyazaki, Yasunari, Masuo, Masahiro, Suhara, Kozo, Tateishi, Tomoya, Yasui, Makito, Inase, Naohiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569356/
https://www.ncbi.nlm.nih.gov/pubmed/26367130
http://dx.doi.org/10.1371/journal.pone.0137978
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author Ishizuka, Masahiro
Miyazaki, Yasunari
Masuo, Masahiro
Suhara, Kozo
Tateishi, Tomoya
Yasui, Makito
Inase, Naohiko
author_facet Ishizuka, Masahiro
Miyazaki, Yasunari
Masuo, Masahiro
Suhara, Kozo
Tateishi, Tomoya
Yasui, Makito
Inase, Naohiko
author_sort Ishizuka, Masahiro
collection PubMed
description Hypersensitivity pneumonitis (HP) is an immune mediated lung disease induced by the repeated inhalation of a wide variety of antigens. Bird-related hypersensitivity pneumonitis (BRHP) is one of the most common forms of HP in human and results from the inhalation of avian antigens. The findings of a recent clinical analysis suggest that in addition to Th1 factors, the levels of interleukin(IL)-17 and IL-17-associated transcripts are increased in the setting of HP, and that both IL-17A and neutrophils are crucial for the development of pulmonary inflammation in murine models of HP. Our objectives were to investigate the roles of IL-17A and neutrophils in granuloma-forming inflammation in an acute HP model. We developed a mouse model of acute BRHP using pigeon dropping extract. We evaluated the process of granuloma formation and the roles of both IL-17A and neutrophils in a model. We found that the neutralization of IL-17A by the antibody attenuated granuloma formation and the recruitment of neutrophils, and also decreased the expression level of chemokine(C-X-C motif) ligand 5 (CXCL5) in the acute HP model. We confirmed that most of the neutrophils in the acute HP model exhibited immunoreactivity to the anti-IL-17 antibody. We have identified the central roles of both IL-17A and neutrophils in the pathogenesis of granuloma formation in acute HP. We have also assumed that neutrophils are an important source of IL-17A in an acute HP model, and that the IL-17A-CXCL5 pathway may be responsible for the recruitment of neutrophils.
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spelling pubmed-45693562015-09-18 Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis Ishizuka, Masahiro Miyazaki, Yasunari Masuo, Masahiro Suhara, Kozo Tateishi, Tomoya Yasui, Makito Inase, Naohiko PLoS One Research Article Hypersensitivity pneumonitis (HP) is an immune mediated lung disease induced by the repeated inhalation of a wide variety of antigens. Bird-related hypersensitivity pneumonitis (BRHP) is one of the most common forms of HP in human and results from the inhalation of avian antigens. The findings of a recent clinical analysis suggest that in addition to Th1 factors, the levels of interleukin(IL)-17 and IL-17-associated transcripts are increased in the setting of HP, and that both IL-17A and neutrophils are crucial for the development of pulmonary inflammation in murine models of HP. Our objectives were to investigate the roles of IL-17A and neutrophils in granuloma-forming inflammation in an acute HP model. We developed a mouse model of acute BRHP using pigeon dropping extract. We evaluated the process of granuloma formation and the roles of both IL-17A and neutrophils in a model. We found that the neutralization of IL-17A by the antibody attenuated granuloma formation and the recruitment of neutrophils, and also decreased the expression level of chemokine(C-X-C motif) ligand 5 (CXCL5) in the acute HP model. We confirmed that most of the neutrophils in the acute HP model exhibited immunoreactivity to the anti-IL-17 antibody. We have identified the central roles of both IL-17A and neutrophils in the pathogenesis of granuloma formation in acute HP. We have also assumed that neutrophils are an important source of IL-17A in an acute HP model, and that the IL-17A-CXCL5 pathway may be responsible for the recruitment of neutrophils. Public Library of Science 2015-09-14 /pmc/articles/PMC4569356/ /pubmed/26367130 http://dx.doi.org/10.1371/journal.pone.0137978 Text en © 2015 Ishizuka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ishizuka, Masahiro
Miyazaki, Yasunari
Masuo, Masahiro
Suhara, Kozo
Tateishi, Tomoya
Yasui, Makito
Inase, Naohiko
Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis
title Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis
title_full Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis
title_fullStr Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis
title_full_unstemmed Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis
title_short Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis
title_sort interleukin-17a and neutrophils in a murine model of bird-related hypersensitivity pneumonitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569356/
https://www.ncbi.nlm.nih.gov/pubmed/26367130
http://dx.doi.org/10.1371/journal.pone.0137978
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