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Transcriptome Analysis Reveals Key Flavonoid 3′-Hydroxylase and Flavonoid 3′,5′-Hydroxylase Genes in Affecting the Ratio of Dihydroxylated to Trihydroxylated Catechins in Camellia sinensis

The ratio of dihydroxylated to trihydroxylated catechins (RDTC) is an important indicator of tea quality and biochemical marker for the study of genetic diversity. It is reported to be under genetic control but the underlying mechanism is not well understood. Flavonoid 3′-hydroxylase (F3′H) and flav...

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Autores principales: Wei, Kang, Wang, Liyuan, Zhang, Chengcai, Wu, Liyun, Li, Hailin, Zhang, Fen, Cheng, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569414/
https://www.ncbi.nlm.nih.gov/pubmed/26367395
http://dx.doi.org/10.1371/journal.pone.0137925
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author Wei, Kang
Wang, Liyuan
Zhang, Chengcai
Wu, Liyun
Li, Hailin
Zhang, Fen
Cheng, Hao
author_facet Wei, Kang
Wang, Liyuan
Zhang, Chengcai
Wu, Liyun
Li, Hailin
Zhang, Fen
Cheng, Hao
author_sort Wei, Kang
collection PubMed
description The ratio of dihydroxylated to trihydroxylated catechins (RDTC) is an important indicator of tea quality and biochemical marker for the study of genetic diversity. It is reported to be under genetic control but the underlying mechanism is not well understood. Flavonoid 3′-hydroxylase (F3′H) and flavonoid 3′,5′-hydroxylase (F3′5′H) are key enzymes involved in the formation of dihydroxylated and trihydroxylated catechins. The transcriptome and HPLC analysis of tea samples from Longjing43 and Zhonghuang2 under control and shading treatment were performed to assess the F3′H and F3′5′H genes that might affect RDTC. A total of 74.7 million reads of mRNA seq (2×101bp) data were generated. After de novo assembly, 109,909 unigenes were obtained, and 39,982 of them were annotated using 7 public databases. Four key F3′H and F3′5′H genes (including CsF3′5′H1, CsF3′H1, CsF3′H2 and CsF3′H3) were identified to be closely correlated with RDTC. Shading treatment had little effect on RDTC, which was attributed to the stable expression of these key F3′H and F3′5′H genes. The correlation of the coexpression of four key genes and RDTC was further confirmed among 13 tea varieties by real time PCR and HPLC analysis. The coexpression of three F3′H genes and a F3′5′H gene may play a key role in affecting RDTC in Camellia sinensis. The current results may establish valuable foundation for further research about the mechanism controlling catechin composition in tea.
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spelling pubmed-45694142015-09-18 Transcriptome Analysis Reveals Key Flavonoid 3′-Hydroxylase and Flavonoid 3′,5′-Hydroxylase Genes in Affecting the Ratio of Dihydroxylated to Trihydroxylated Catechins in Camellia sinensis Wei, Kang Wang, Liyuan Zhang, Chengcai Wu, Liyun Li, Hailin Zhang, Fen Cheng, Hao PLoS One Research Article The ratio of dihydroxylated to trihydroxylated catechins (RDTC) is an important indicator of tea quality and biochemical marker for the study of genetic diversity. It is reported to be under genetic control but the underlying mechanism is not well understood. Flavonoid 3′-hydroxylase (F3′H) and flavonoid 3′,5′-hydroxylase (F3′5′H) are key enzymes involved in the formation of dihydroxylated and trihydroxylated catechins. The transcriptome and HPLC analysis of tea samples from Longjing43 and Zhonghuang2 under control and shading treatment were performed to assess the F3′H and F3′5′H genes that might affect RDTC. A total of 74.7 million reads of mRNA seq (2×101bp) data were generated. After de novo assembly, 109,909 unigenes were obtained, and 39,982 of them were annotated using 7 public databases. Four key F3′H and F3′5′H genes (including CsF3′5′H1, CsF3′H1, CsF3′H2 and CsF3′H3) were identified to be closely correlated with RDTC. Shading treatment had little effect on RDTC, which was attributed to the stable expression of these key F3′H and F3′5′H genes. The correlation of the coexpression of four key genes and RDTC was further confirmed among 13 tea varieties by real time PCR and HPLC analysis. The coexpression of three F3′H genes and a F3′5′H gene may play a key role in affecting RDTC in Camellia sinensis. The current results may establish valuable foundation for further research about the mechanism controlling catechin composition in tea. Public Library of Science 2015-09-14 /pmc/articles/PMC4569414/ /pubmed/26367395 http://dx.doi.org/10.1371/journal.pone.0137925 Text en © 2015 Wei et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wei, Kang
Wang, Liyuan
Zhang, Chengcai
Wu, Liyun
Li, Hailin
Zhang, Fen
Cheng, Hao
Transcriptome Analysis Reveals Key Flavonoid 3′-Hydroxylase and Flavonoid 3′,5′-Hydroxylase Genes in Affecting the Ratio of Dihydroxylated to Trihydroxylated Catechins in Camellia sinensis
title Transcriptome Analysis Reveals Key Flavonoid 3′-Hydroxylase and Flavonoid 3′,5′-Hydroxylase Genes in Affecting the Ratio of Dihydroxylated to Trihydroxylated Catechins in Camellia sinensis
title_full Transcriptome Analysis Reveals Key Flavonoid 3′-Hydroxylase and Flavonoid 3′,5′-Hydroxylase Genes in Affecting the Ratio of Dihydroxylated to Trihydroxylated Catechins in Camellia sinensis
title_fullStr Transcriptome Analysis Reveals Key Flavonoid 3′-Hydroxylase and Flavonoid 3′,5′-Hydroxylase Genes in Affecting the Ratio of Dihydroxylated to Trihydroxylated Catechins in Camellia sinensis
title_full_unstemmed Transcriptome Analysis Reveals Key Flavonoid 3′-Hydroxylase and Flavonoid 3′,5′-Hydroxylase Genes in Affecting the Ratio of Dihydroxylated to Trihydroxylated Catechins in Camellia sinensis
title_short Transcriptome Analysis Reveals Key Flavonoid 3′-Hydroxylase and Flavonoid 3′,5′-Hydroxylase Genes in Affecting the Ratio of Dihydroxylated to Trihydroxylated Catechins in Camellia sinensis
title_sort transcriptome analysis reveals key flavonoid 3′-hydroxylase and flavonoid 3′,5′-hydroxylase genes in affecting the ratio of dihydroxylated to trihydroxylated catechins in camellia sinensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569414/
https://www.ncbi.nlm.nih.gov/pubmed/26367395
http://dx.doi.org/10.1371/journal.pone.0137925
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