Comparative studies for the biotechnological production of l-Lysine by immobilized cells of wild-type Corynebacterium glutamicum ATCC 13032 and mutant MH 20-22 B

Establishing a cost and time efficient approach for bioprocess optimization is desired but is challenging. In the present work, we have addressed the effectiveness of using immobilized cells for aerobic processes, behaviour of immobilized cells, optimization and upstream bioprocess analysis for the production of lysine by immobilized cells of Corynebacterium glutamicum ATCC 13032 and MH 20-22 B in stirred tank bioreactor. Optimized operational conditions for maximal yield and productivity were determined with six parameters i.e., pH, temperature, fermentation time, airflow rate, glucose concentration and aeration rate. With the obtained results, it was evident that the optimum values for the upstream parameters viz., fermentation time, pH, temperature, glucose concentration, air flow rate and agitation rate are 96 h, 7.5, 30 °C, 90 g/l, 1.0 vvm and 200 rpm for both immobilized cells of C. glutamicum ATCC 13032 and MH 20-22 B. Immobilized cells of C. glutamicum MH 20-22 B, which is a leucine auxotroph has yielded more l-lysine compare to the immobilized cells of wild type strain C. glutamicum ATCC 13032.