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T-cell receptor Vβ repertoire of CD8(+) T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil

In human cutaneous leishmaniasis (CL), the immune response is mainly mediated by T-cells. The role of CD8(+) T-lymphocytes, which are related to healing or deleterious functions, in affecting clinical outcome is controversial. The aim of this study was to evaluate T-cell receptor diversity in late-d...

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Autores principales: Ferraz, Raquel, Cunha, Clarissa Ferreira, Pimentel, Maria Inês, Lyra, Marcelo Rosandiski, Schubach, Armando Oliveira, de Mendonça, Sérgio Coutinho Furtado, Da-Cruz, Alda Maria, Bertho, Alvaro Luiz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569821/
https://www.ncbi.nlm.nih.gov/pubmed/26107186
http://dx.doi.org/10.1590/0074-02760150039
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author Ferraz, Raquel
Cunha, Clarissa Ferreira
Pimentel, Maria Inês
Lyra, Marcelo Rosandiski
Schubach, Armando Oliveira
de Mendonça, Sérgio Coutinho Furtado
Da-Cruz, Alda Maria
Bertho, Alvaro Luiz
author_facet Ferraz, Raquel
Cunha, Clarissa Ferreira
Pimentel, Maria Inês
Lyra, Marcelo Rosandiski
Schubach, Armando Oliveira
de Mendonça, Sérgio Coutinho Furtado
Da-Cruz, Alda Maria
Bertho, Alvaro Luiz
author_sort Ferraz, Raquel
collection PubMed
description In human cutaneous leishmaniasis (CL), the immune response is mainly mediated by T-cells. The role of CD8(+) T-lymphocytes, which are related to healing or deleterious functions, in affecting clinical outcome is controversial. The aim of this study was to evaluate T-cell receptor diversity in late-differentiated effector (LDE) and memory CD8(+) T-cell subsets in order to create a profile of specific clones engaged in deleterious or protective CL immune responses. Healthy subjects, patients with active disease (PAD) and clinically cured patients were enrolled in the study. Total CD8(+) T-lymphocytes showed a disturbance in the expression of the Vβ2, Vβ9, Vβ13.2, Vβ18 and Vβ23 families. The analyses of CD8(+)T-lymphocyte subsets showed high frequencies of LDE CD8(+)T-lymphocytes expressing Vβ12 and Vβ22 in PAD, as well as effector-memory CD8(+) T-cells expressing Vβ22. We also observed low frequencies of effector and central-memory CD8(+) T-cells expressing Vβ2 in PAD, which correlated with a greater lesion size. Particular Vβ expansions point to CD8(+) T-cell clones that are selected during CL immune responses, suggesting that CD8(+) T-lymphocytes expressing Vβ12 or Vβ22 are involved in a LDE response and that Vβ2 contractions in memory CD8(+)T-cells are associated with larger lesions.
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spelling pubmed-45698212015-09-15 T-cell receptor Vβ repertoire of CD8(+) T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil Ferraz, Raquel Cunha, Clarissa Ferreira Pimentel, Maria Inês Lyra, Marcelo Rosandiski Schubach, Armando Oliveira de Mendonça, Sérgio Coutinho Furtado Da-Cruz, Alda Maria Bertho, Alvaro Luiz Mem Inst Oswaldo Cruz Articles In human cutaneous leishmaniasis (CL), the immune response is mainly mediated by T-cells. The role of CD8(+) T-lymphocytes, which are related to healing or deleterious functions, in affecting clinical outcome is controversial. The aim of this study was to evaluate T-cell receptor diversity in late-differentiated effector (LDE) and memory CD8(+) T-cell subsets in order to create a profile of specific clones engaged in deleterious or protective CL immune responses. Healthy subjects, patients with active disease (PAD) and clinically cured patients were enrolled in the study. Total CD8(+) T-lymphocytes showed a disturbance in the expression of the Vβ2, Vβ9, Vβ13.2, Vβ18 and Vβ23 families. The analyses of CD8(+)T-lymphocyte subsets showed high frequencies of LDE CD8(+)T-lymphocytes expressing Vβ12 and Vβ22 in PAD, as well as effector-memory CD8(+) T-cells expressing Vβ22. We also observed low frequencies of effector and central-memory CD8(+) T-cells expressing Vβ2 in PAD, which correlated with a greater lesion size. Particular Vβ expansions point to CD8(+) T-cell clones that are selected during CL immune responses, suggesting that CD8(+) T-lymphocytes expressing Vβ12 or Vβ22 are involved in a LDE response and that Vβ2 contractions in memory CD8(+)T-cells are associated with larger lesions. Instituto Oswaldo Cruz, Ministério da Saúde 2015-08 /pmc/articles/PMC4569821/ /pubmed/26107186 http://dx.doi.org/10.1590/0074-02760150039 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Ferraz, Raquel
Cunha, Clarissa Ferreira
Pimentel, Maria Inês
Lyra, Marcelo Rosandiski
Schubach, Armando Oliveira
de Mendonça, Sérgio Coutinho Furtado
Da-Cruz, Alda Maria
Bertho, Alvaro Luiz
T-cell receptor Vβ repertoire of CD8(+) T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil
title T-cell receptor Vβ repertoire of CD8(+) T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil
title_full T-cell receptor Vβ repertoire of CD8(+) T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil
title_fullStr T-cell receptor Vβ repertoire of CD8(+) T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil
title_full_unstemmed T-cell receptor Vβ repertoire of CD8(+) T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil
title_short T-cell receptor Vβ repertoire of CD8(+) T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil
title_sort t-cell receptor vβ repertoire of cd8(+) t-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of rio de janeiro, brazil
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569821/
https://www.ncbi.nlm.nih.gov/pubmed/26107186
http://dx.doi.org/10.1590/0074-02760150039
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