Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour cri...

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Detalles Bibliográficos
Autores principales: Santos, Jefferson José da Silva, Magalhães, Tereza, Silva, José Valter Joaquim, da Silva, Andréa Nazaré Monteiro Rangel, Cordeiro, Marli Tenório, Gil, Laura Helena Vega Gonzales
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569833/
https://www.ncbi.nlm.nih.gov/pubmed/26200712
http://dx.doi.org/10.1590/0074-02760150053
Descripción
Sumario:Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.

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