Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour cri...

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Autores principales: Santos, Jefferson José da Silva, Magalhães, Tereza, Silva, José Valter Joaquim, da Silva, Andréa Nazaré Monteiro Rangel, Cordeiro, Marli Tenório, Gil, Laura Helena Vega Gonzales
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569833/
https://www.ncbi.nlm.nih.gov/pubmed/26200712
http://dx.doi.org/10.1590/0074-02760150053
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author Santos, Jefferson José da Silva
Magalhães, Tereza
Silva, José Valter Joaquim
da Silva, Andréa Nazaré Monteiro Rangel
Cordeiro, Marli Tenório
Gil, Laura Helena Vega Gonzales
author_facet Santos, Jefferson José da Silva
Magalhães, Tereza
Silva, José Valter Joaquim
da Silva, Andréa Nazaré Monteiro Rangel
Cordeiro, Marli Tenório
Gil, Laura Helena Vega Gonzales
author_sort Santos, Jefferson José da Silva
collection PubMed
description Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.
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spelling pubmed-45698332015-09-15 Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil Santos, Jefferson José da Silva Magalhães, Tereza Silva, José Valter Joaquim da Silva, Andréa Nazaré Monteiro Rangel Cordeiro, Marli Tenório Gil, Laura Helena Vega Gonzales Mem Inst Oswaldo Cruz Short Communication Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons. Instituto Oswaldo Cruz, Ministério da Saúde 2015-08 /pmc/articles/PMC4569833/ /pubmed/26200712 http://dx.doi.org/10.1590/0074-02760150053 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Santos, Jefferson José da Silva
Magalhães, Tereza
Silva, José Valter Joaquim
da Silva, Andréa Nazaré Monteiro Rangel
Cordeiro, Marli Tenório
Gil, Laura Helena Vega Gonzales
Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_full Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_fullStr Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_full_unstemmed Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_short Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
title_sort full-length infectious clone of a low passage dengue virus serotype 2 from brazil
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569833/
https://www.ncbi.nlm.nih.gov/pubmed/26200712
http://dx.doi.org/10.1590/0074-02760150053
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