Cargando…
RIG-I contributes to the innate immune response after cerebral ischemia
BACKGROUND: Focal cerebral ischemia induces an inflammatory response that when exacerbated contributes to deleterious outcomes. The molecular basis regarding the regulation of the innate immune response after focal cerebral ischemia remains poorly understood. METHODS: In this study we examined the e...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570046/ https://www.ncbi.nlm.nih.gov/pubmed/26379474 http://dx.doi.org/10.1186/s12950-015-0101-4 |
_version_ | 1782390150864568320 |
---|---|
author | Brand, Frank J. de Rivero Vaccari, Juan Carlos Mejias, Nancy H. Alonso, Ofelia F. de Rivero Vaccari, Juan Pablo |
author_facet | Brand, Frank J. de Rivero Vaccari, Juan Carlos Mejias, Nancy H. Alonso, Ofelia F. de Rivero Vaccari, Juan Pablo |
author_sort | Brand, Frank J. |
collection | PubMed |
description | BACKGROUND: Focal cerebral ischemia induces an inflammatory response that when exacerbated contributes to deleterious outcomes. The molecular basis regarding the regulation of the innate immune response after focal cerebral ischemia remains poorly understood. METHODS: In this study we examined the expression of retinoic acid-inducible gene (RIG)-like receptor-I (RIG-I) and its involvement in regulating inflammation after ischemia in the brain of rats subjected to middle cerebral artery occlusion (MCAO). In addition, we studied the regulation of RIG-I after oxygen glucose deprivation (OGD) in astrocytes in culture. RESULTS: In this study we show that in the hippocampus of rats, RIG-I and IFN-α are elevated after MCAO. Consistent with these results was an increased in RIG-I and IFN-α after OGD in astrocytes in culture. These data are consistent with immunohistochemical analysis of hippocampal sections, indicating that in GFAP-positive cells there was an increase in RIG-I after MCAO. In addition, in this study we have identified n-propyl gallate as an inhibitor of IFN-α signaling in astrocytes. CONCLUSION: Our findings suggest a role for RIG-I in contributing to the innate immune response after focal cerebral ischemia. |
format | Online Article Text |
id | pubmed-4570046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45700462015-09-16 RIG-I contributes to the innate immune response after cerebral ischemia Brand, Frank J. de Rivero Vaccari, Juan Carlos Mejias, Nancy H. Alonso, Ofelia F. de Rivero Vaccari, Juan Pablo J Inflamm (Lond) Research BACKGROUND: Focal cerebral ischemia induces an inflammatory response that when exacerbated contributes to deleterious outcomes. The molecular basis regarding the regulation of the innate immune response after focal cerebral ischemia remains poorly understood. METHODS: In this study we examined the expression of retinoic acid-inducible gene (RIG)-like receptor-I (RIG-I) and its involvement in regulating inflammation after ischemia in the brain of rats subjected to middle cerebral artery occlusion (MCAO). In addition, we studied the regulation of RIG-I after oxygen glucose deprivation (OGD) in astrocytes in culture. RESULTS: In this study we show that in the hippocampus of rats, RIG-I and IFN-α are elevated after MCAO. Consistent with these results was an increased in RIG-I and IFN-α after OGD in astrocytes in culture. These data are consistent with immunohistochemical analysis of hippocampal sections, indicating that in GFAP-positive cells there was an increase in RIG-I after MCAO. In addition, in this study we have identified n-propyl gallate as an inhibitor of IFN-α signaling in astrocytes. CONCLUSION: Our findings suggest a role for RIG-I in contributing to the innate immune response after focal cerebral ischemia. BioMed Central 2015-09-14 /pmc/articles/PMC4570046/ /pubmed/26379474 http://dx.doi.org/10.1186/s12950-015-0101-4 Text en © Brand et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Brand, Frank J. de Rivero Vaccari, Juan Carlos Mejias, Nancy H. Alonso, Ofelia F. de Rivero Vaccari, Juan Pablo RIG-I contributes to the innate immune response after cerebral ischemia |
title | RIG-I contributes to the innate immune response after cerebral ischemia |
title_full | RIG-I contributes to the innate immune response after cerebral ischemia |
title_fullStr | RIG-I contributes to the innate immune response after cerebral ischemia |
title_full_unstemmed | RIG-I contributes to the innate immune response after cerebral ischemia |
title_short | RIG-I contributes to the innate immune response after cerebral ischemia |
title_sort | rig-i contributes to the innate immune response after cerebral ischemia |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570046/ https://www.ncbi.nlm.nih.gov/pubmed/26379474 http://dx.doi.org/10.1186/s12950-015-0101-4 |
work_keys_str_mv | AT brandfrankj rigicontributestotheinnateimmuneresponseaftercerebralischemia AT deriverovaccarijuancarlos rigicontributestotheinnateimmuneresponseaftercerebralischemia AT mejiasnancyh rigicontributestotheinnateimmuneresponseaftercerebralischemia AT alonsoofeliaf rigicontributestotheinnateimmuneresponseaftercerebralischemia AT deriverovaccarijuanpablo rigicontributestotheinnateimmuneresponseaftercerebralischemia |