The proteome of mouse vestibular hair bundles over development

Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundl...

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Autores principales: Krey, Jocelyn F., Sherman, Nicholas E., Jeffery, Erin D, Choi, Dongseok, Barr-Gillespie, Peter G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570149/
https://www.ncbi.nlm.nih.gov/pubmed/26401315
http://dx.doi.org/10.1038/sdata.2015.47
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author Krey, Jocelyn F.
Sherman, Nicholas E.
Jeffery, Erin D
Choi, Dongseok
Barr-Gillespie, Peter G.
author_facet Krey, Jocelyn F.
Sherman, Nicholas E.
Jeffery, Erin D
Choi, Dongseok
Barr-Gillespie, Peter G.
author_sort Krey, Jocelyn F.
collection PubMed
description Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using MaxQuant and label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricle; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data, which are available via ProteomeXchange with identifier PXD002167, will be useful for examining the proteins present in mammalian vestibular bundles and how their concentrations change over development.
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spelling pubmed-45701492015-09-23 The proteome of mouse vestibular hair bundles over development Krey, Jocelyn F. Sherman, Nicholas E. Jeffery, Erin D Choi, Dongseok Barr-Gillespie, Peter G. Sci Data Data Descriptor Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using MaxQuant and label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricle; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data, which are available via ProteomeXchange with identifier PXD002167, will be useful for examining the proteins present in mammalian vestibular bundles and how their concentrations change over development. Nature Publishing Group 2015-09-15 /pmc/articles/PMC4570149/ /pubmed/26401315 http://dx.doi.org/10.1038/sdata.2015.47 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse.
spellingShingle Data Descriptor
Krey, Jocelyn F.
Sherman, Nicholas E.
Jeffery, Erin D
Choi, Dongseok
Barr-Gillespie, Peter G.
The proteome of mouse vestibular hair bundles over development
title The proteome of mouse vestibular hair bundles over development
title_full The proteome of mouse vestibular hair bundles over development
title_fullStr The proteome of mouse vestibular hair bundles over development
title_full_unstemmed The proteome of mouse vestibular hair bundles over development
title_short The proteome of mouse vestibular hair bundles over development
title_sort proteome of mouse vestibular hair bundles over development
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570149/
https://www.ncbi.nlm.nih.gov/pubmed/26401315
http://dx.doi.org/10.1038/sdata.2015.47
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