Cargando…

Electrochemical Characterization of Protein Adsorption onto YNGRT-Au and VLGXE-Au Surfaces

The adsorption of the proteins CD13, mucin and bovine serum albumin on VLGXE-Au and YNGRT-Au interfaces was monitored by electrochemical impedance spectroscopy in the presence of [Fe(CN)(6)](3−/4−). The hydrophobicity of the Au surface was tailored using specific peptides, blocking agents and diluen...

Descripción completa

Detalles Bibliográficos
Autores principales: Trzeciakiewicz, Hanna, Esteves-Villanueva, Jose, Soudy, Rania, Kaur, Kamaljit, Martic-Milne, Sanela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570378/
https://www.ncbi.nlm.nih.gov/pubmed/26262621
http://dx.doi.org/10.3390/s150819429
Descripción
Sumario:The adsorption of the proteins CD13, mucin and bovine serum albumin on VLGXE-Au and YNGRT-Au interfaces was monitored by electrochemical impedance spectroscopy in the presence of [Fe(CN)(6)](3−/4−). The hydrophobicity of the Au surface was tailored using specific peptides, blocking agents and diluents. The combination of blocking agents (ethanolamine or n-butylamine) and diluents (hexanethiol or 2-mercaptoethanol) was used to prepare various peptide-modified Au surfaces. Protein adsorption onto the peptide-Au surfaces modified with the combination of n-butylamine and hexanethiol produced a dramatic decrease in the charge transfer resistance, R(ct), for all three proteins. In contrast, polar peptide-surfaces induced a minimal change in R(ct) for all three proteins. Furthermore, an increase in R(ct) was observed with CD13 (an aminopeptidase overexpressed in certain cancers) in comparison to the other proteins when the VLGXE-Au surface was modified with n-butylamine as a blocking agent. The electrochemical data indicated that protein adsorption may be modulated by tailoring the peptide sequence on Au surfaces and that blocking agents and diluents play a key role in promoting or preventing protein adsorption. The peptide-Au platform may also be used for targeting cancer biomarkers with designer peptides.