Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique

The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested fo...

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Autores principales: Laguna, Maríafe, Holgado, Miguel, Hernandez, Ana L., Santamaría, Beatriz, Lavín, Alvaro, Soria, Javier, Suarez, Tatiana, Bardina, Carlota, Jara, Mónica, Sanza, Francisco J., Casquel, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570398/
https://www.ncbi.nlm.nih.gov/pubmed/26287192
http://dx.doi.org/10.3390/s150819819
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author Laguna, Maríafe
Holgado, Miguel
Hernandez, Ana L.
Santamaría, Beatriz
Lavín, Alvaro
Soria, Javier
Suarez, Tatiana
Bardina, Carlota
Jara, Mónica
Sanza, Francisco J.
Casquel, Rafael
author_facet Laguna, Maríafe
Holgado, Miguel
Hernandez, Ana L.
Santamaría, Beatriz
Lavín, Alvaro
Soria, Javier
Suarez, Tatiana
Bardina, Carlota
Jara, Mónica
Sanza, Francisco J.
Casquel, Rafael
author_sort Laguna, Maríafe
collection PubMed
description The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.
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spelling pubmed-45703982015-09-17 Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique Laguna, Maríafe Holgado, Miguel Hernandez, Ana L. Santamaría, Beatriz Lavín, Alvaro Soria, Javier Suarez, Tatiana Bardina, Carlota Jara, Mónica Sanza, Francisco J. Casquel, Rafael Sensors (Basel) Article The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique. MDPI 2015-08-13 /pmc/articles/PMC4570398/ /pubmed/26287192 http://dx.doi.org/10.3390/s150819819 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Laguna, Maríafe
Holgado, Miguel
Hernandez, Ana L.
Santamaría, Beatriz
Lavín, Alvaro
Soria, Javier
Suarez, Tatiana
Bardina, Carlota
Jara, Mónica
Sanza, Francisco J.
Casquel, Rafael
Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
title Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
title_full Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
title_fullStr Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
title_full_unstemmed Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
title_short Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
title_sort antigen-antibody affinity for dry eye biomarkers by label free biosensing. comparison with the elisa technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570398/
https://www.ncbi.nlm.nih.gov/pubmed/26287192
http://dx.doi.org/10.3390/s150819819
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