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Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life

Nicotine exposure has been associated with an increased likelihood of developing attention deficit hyperactivity disorder (ADHD) in offspring of mothers who smoked during pregnancy. The goal of this study was to determine if exposure to E-cigarette nicotine vapors during late prenatal and early post...

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Autores principales: Smith, Dani, Aherrera, Angela, Lopez, Armando, Neptune, Enid, Winickoff, Jonathan P., Klein, Jonathan D., Chen, Gang, Lazarus, Philip, Collaco, Joseph M., McGrath-Morrow, Sharon A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570802/
https://www.ncbi.nlm.nih.gov/pubmed/26372012
http://dx.doi.org/10.1371/journal.pone.0137953
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author Smith, Dani
Aherrera, Angela
Lopez, Armando
Neptune, Enid
Winickoff, Jonathan P.
Klein, Jonathan D.
Chen, Gang
Lazarus, Philip
Collaco, Joseph M.
McGrath-Morrow, Sharon A.
author_facet Smith, Dani
Aherrera, Angela
Lopez, Armando
Neptune, Enid
Winickoff, Jonathan P.
Klein, Jonathan D.
Chen, Gang
Lazarus, Philip
Collaco, Joseph M.
McGrath-Morrow, Sharon A.
author_sort Smith, Dani
collection PubMed
description Nicotine exposure has been associated with an increased likelihood of developing attention deficit hyperactivity disorder (ADHD) in offspring of mothers who smoked during pregnancy. The goal of this study was to determine if exposure to E-cigarette nicotine vapors during late prenatal and early postnatal life altered behavior in adult mice. METHODS: Timed-pregnant C57BL/6J mice were exposed to 2.4% nicotine in propylene glycol (PG) or 0% nicotine /PG once a day from gestational day 15 until delivery. After delivery, offspring and mothers were exposed to E-cigarette vapors for an additional 14 days from postnatal day 2 through 16. Following their last exposure serum cotinine levels were measured in female juvenile mice. Male mice underwent behavioral testing at 14 weeks of age to assess sensorimotor, affective, and cognitive functional domains. RESULTS: Adult male mice exposed to 2.4% nicotine/PG E-cigarette vapors had significantly more head dips in the zero maze test and higher levels of rearing activity in the open field test compared to 0% nicotine/PG exposed mice and untreated controls. In the water maze test after reversal training, the 2.4% nicotine/PG mice spent more than 25% of time in the new location whereas the other groups did not. CONCLUSION: Adult male mice exhibited increased levels of activity in the zero maze and open field tests when exposed to E-cigarette vapor containing nicotine during late prenatal and early postnatal life. These findings indicate that nicotine exposure from E-cigarettes may cause persistent behavioral changes when exposure occurs during a period of rapid brain growth.
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spelling pubmed-45708022015-09-18 Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life Smith, Dani Aherrera, Angela Lopez, Armando Neptune, Enid Winickoff, Jonathan P. Klein, Jonathan D. Chen, Gang Lazarus, Philip Collaco, Joseph M. McGrath-Morrow, Sharon A. PLoS One Research Article Nicotine exposure has been associated with an increased likelihood of developing attention deficit hyperactivity disorder (ADHD) in offspring of mothers who smoked during pregnancy. The goal of this study was to determine if exposure to E-cigarette nicotine vapors during late prenatal and early postnatal life altered behavior in adult mice. METHODS: Timed-pregnant C57BL/6J mice were exposed to 2.4% nicotine in propylene glycol (PG) or 0% nicotine /PG once a day from gestational day 15 until delivery. After delivery, offspring and mothers were exposed to E-cigarette vapors for an additional 14 days from postnatal day 2 through 16. Following their last exposure serum cotinine levels were measured in female juvenile mice. Male mice underwent behavioral testing at 14 weeks of age to assess sensorimotor, affective, and cognitive functional domains. RESULTS: Adult male mice exposed to 2.4% nicotine/PG E-cigarette vapors had significantly more head dips in the zero maze test and higher levels of rearing activity in the open field test compared to 0% nicotine/PG exposed mice and untreated controls. In the water maze test after reversal training, the 2.4% nicotine/PG mice spent more than 25% of time in the new location whereas the other groups did not. CONCLUSION: Adult male mice exhibited increased levels of activity in the zero maze and open field tests when exposed to E-cigarette vapor containing nicotine during late prenatal and early postnatal life. These findings indicate that nicotine exposure from E-cigarettes may cause persistent behavioral changes when exposure occurs during a period of rapid brain growth. Public Library of Science 2015-09-15 /pmc/articles/PMC4570802/ /pubmed/26372012 http://dx.doi.org/10.1371/journal.pone.0137953 Text en © 2015 Smith et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Smith, Dani
Aherrera, Angela
Lopez, Armando
Neptune, Enid
Winickoff, Jonathan P.
Klein, Jonathan D.
Chen, Gang
Lazarus, Philip
Collaco, Joseph M.
McGrath-Morrow, Sharon A.
Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life
title Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life
title_full Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life
title_fullStr Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life
title_full_unstemmed Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life
title_short Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life
title_sort adult behavior in male mice exposed to e-cigarette nicotine vapors during late prenatal and early postnatal life
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570802/
https://www.ncbi.nlm.nih.gov/pubmed/26372012
http://dx.doi.org/10.1371/journal.pone.0137953
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