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Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice
BACKGROUND: To examine the circulating microRNA (miRNA) expression profile in a mouse model of diet-induced obesity (DIO) with subsequent weight reduction achieved via low-fat diet (LFD) feeding. RESULTS: Eighteen C57BL/6NCrl male mice were divided into three subgroups: (1) control, mice were fed a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571067/ https://www.ncbi.nlm.nih.gov/pubmed/26377847 http://dx.doi.org/10.1186/s12864-015-1896-3 |
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author | Hsieh, Ching-Hua Rau, Cheng-Shyuan Wu, Shao-Chun Yang, Johnson Chia-Shen Wu, Yi-Chan Lu, Tsu-Hsiang Tzeng, Siou-Ling Wu, Chia-Jung Lin, Chia-Wei |
author_facet | Hsieh, Ching-Hua Rau, Cheng-Shyuan Wu, Shao-Chun Yang, Johnson Chia-Shen Wu, Yi-Chan Lu, Tsu-Hsiang Tzeng, Siou-Ling Wu, Chia-Jung Lin, Chia-Wei |
author_sort | Hsieh, Ching-Hua |
collection | PubMed |
description | BACKGROUND: To examine the circulating microRNA (miRNA) expression profile in a mouse model of diet-induced obesity (DIO) with subsequent weight reduction achieved via low-fat diet (LFD) feeding. RESULTS: Eighteen C57BL/6NCrl male mice were divided into three subgroups: (1) control, mice were fed a standard AIN-76A (fat: 11.5 kcal %) diet for 12 weeks; (2) DIO, mice were fed a 58 kcal % high-fat diet (HFD) for 12 weeks; and (3) DIO + LFD, mice were fed a HFD for 8 weeks to induce obesity and then switched to a 10.5 kcal % LFD for 4 weeks. A switch to LFD feeding led to decreases in body weight, adiposity, and blood glucose levels in DIO mice. Microarray analysis of miRNA using The Mouse & Rat miRNA OneArray® v4 system revealed significant alterations in the expression of miRNAs in DIO and DIO + LFD mice. Notably, 23 circulating miRNAs (mmu-miR-16, mmu-let-7i, mmu-miR-26a, mmu-miR-17, mmu-miR-107, mmu-miR-195, mmu-miR-20a, mmu-miR-25, mmu-miR-15b, mmu-miR-15a, mmu-let-7b, mmu-let-7a, mmu-let-7c, mmu-miR-103, mmu-let-7f, mmu-miR-106a, mmu-miR-106b, mmu-miR-93, mmu-miR-23b, mmu-miR-21, mmu-miR-30b, mmu-miR-221, and mmu-miR-19b) were significantly downregulated in DIO mice but upregulated in DIO + LFD mice. Target prediction and function annotation of associated genes revealed that these genes were predominantly involved in metabolic, insulin signaling, and adipocytokine signaling pathways that directly link the pathophysiological changes associated with obesity and weight reduction. CONCLUSIONS: These results imply that obesity-related reductions in the expression of circulating miRNAs could be reversed through changes in metabolism associated with weight reduction achieved through LFD feeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-1896-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4571067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45710672015-09-17 Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice Hsieh, Ching-Hua Rau, Cheng-Shyuan Wu, Shao-Chun Yang, Johnson Chia-Shen Wu, Yi-Chan Lu, Tsu-Hsiang Tzeng, Siou-Ling Wu, Chia-Jung Lin, Chia-Wei BMC Genomics Research Article BACKGROUND: To examine the circulating microRNA (miRNA) expression profile in a mouse model of diet-induced obesity (DIO) with subsequent weight reduction achieved via low-fat diet (LFD) feeding. RESULTS: Eighteen C57BL/6NCrl male mice were divided into three subgroups: (1) control, mice were fed a standard AIN-76A (fat: 11.5 kcal %) diet for 12 weeks; (2) DIO, mice were fed a 58 kcal % high-fat diet (HFD) for 12 weeks; and (3) DIO + LFD, mice were fed a HFD for 8 weeks to induce obesity and then switched to a 10.5 kcal % LFD for 4 weeks. A switch to LFD feeding led to decreases in body weight, adiposity, and blood glucose levels in DIO mice. Microarray analysis of miRNA using The Mouse & Rat miRNA OneArray® v4 system revealed significant alterations in the expression of miRNAs in DIO and DIO + LFD mice. Notably, 23 circulating miRNAs (mmu-miR-16, mmu-let-7i, mmu-miR-26a, mmu-miR-17, mmu-miR-107, mmu-miR-195, mmu-miR-20a, mmu-miR-25, mmu-miR-15b, mmu-miR-15a, mmu-let-7b, mmu-let-7a, mmu-let-7c, mmu-miR-103, mmu-let-7f, mmu-miR-106a, mmu-miR-106b, mmu-miR-93, mmu-miR-23b, mmu-miR-21, mmu-miR-30b, mmu-miR-221, and mmu-miR-19b) were significantly downregulated in DIO mice but upregulated in DIO + LFD mice. Target prediction and function annotation of associated genes revealed that these genes were predominantly involved in metabolic, insulin signaling, and adipocytokine signaling pathways that directly link the pathophysiological changes associated with obesity and weight reduction. CONCLUSIONS: These results imply that obesity-related reductions in the expression of circulating miRNAs could be reversed through changes in metabolism associated with weight reduction achieved through LFD feeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-1896-3) contains supplementary material, which is available to authorized users. BioMed Central 2015-09-16 /pmc/articles/PMC4571067/ /pubmed/26377847 http://dx.doi.org/10.1186/s12864-015-1896-3 Text en © Hsieh et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Hsieh, Ching-Hua Rau, Cheng-Shyuan Wu, Shao-Chun Yang, Johnson Chia-Shen Wu, Yi-Chan Lu, Tsu-Hsiang Tzeng, Siou-Ling Wu, Chia-Jung Lin, Chia-Wei Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice |
title | Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice |
title_full | Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice |
title_fullStr | Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice |
title_full_unstemmed | Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice |
title_short | Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice |
title_sort | weight-reduction through a low-fat diet causes differential expression of circulating micrornas in obese c57bl/6 mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571067/ https://www.ncbi.nlm.nih.gov/pubmed/26377847 http://dx.doi.org/10.1186/s12864-015-1896-3 |
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