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Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines
The cell nucleus is a highly organized structure and plays an important role in gene regulation. Understanding the mechanisms that sustain this organization is therefore essential for understanding genome function. Centromeric regions (CRs) of chromosomes have been known for years to adopt specific...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571307/ https://www.ncbi.nlm.nih.gov/pubmed/25947134 http://dx.doi.org/10.1091/mbc.E14-05-1002 |
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author | Ollion, Jean Loll, François Cochennec, Julien Boudier, Thomas Escudé, Christophe |
author_facet | Ollion, Jean Loll, François Cochennec, Julien Boudier, Thomas Escudé, Christophe |
author_sort | Ollion, Jean |
collection | PubMed |
description | The cell nucleus is a highly organized structure and plays an important role in gene regulation. Understanding the mechanisms that sustain this organization is therefore essential for understanding genome function. Centromeric regions (CRs) of chromosomes have been known for years to adopt specific nuclear positioning patterns, but the significance of this observation is not yet completely understood. Here, using a combination of fluorescence in situ hybridization and immunochemistry on fixed human cells and high-throughput imaging, we directly and quantitatively investigated the nuclear positioning of specific human CRs. We observe differential attraction of individual CRs toward both the nuclear border and the nucleoli, the former being enhanced in nonproliferating cells and the latter being enhanced in proliferating cells. Similar positioning patterns are observed in two different lymphoblastoid cell lines. Moreover, the positioning of CRs differs from that of noncentromeric regions, and CRs display specific orientations within chromosome territories. These results suggest the existence of not-yet-characterized mechanisms that drive the nuclear positioning of CRs and therefore pave the way toward a better understanding of how CRs affect nuclear organization. |
format | Online Article Text |
id | pubmed-4571307 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-45713072015-09-29 Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines Ollion, Jean Loll, François Cochennec, Julien Boudier, Thomas Escudé, Christophe Mol Biol Cell Articles The cell nucleus is a highly organized structure and plays an important role in gene regulation. Understanding the mechanisms that sustain this organization is therefore essential for understanding genome function. Centromeric regions (CRs) of chromosomes have been known for years to adopt specific nuclear positioning patterns, but the significance of this observation is not yet completely understood. Here, using a combination of fluorescence in situ hybridization and immunochemistry on fixed human cells and high-throughput imaging, we directly and quantitatively investigated the nuclear positioning of specific human CRs. We observe differential attraction of individual CRs toward both the nuclear border and the nucleoli, the former being enhanced in nonproliferating cells and the latter being enhanced in proliferating cells. Similar positioning patterns are observed in two different lymphoblastoid cell lines. Moreover, the positioning of CRs differs from that of noncentromeric regions, and CRs display specific orientations within chromosome territories. These results suggest the existence of not-yet-characterized mechanisms that drive the nuclear positioning of CRs and therefore pave the way toward a better understanding of how CRs affect nuclear organization. The American Society for Cell Biology 2015-07-01 /pmc/articles/PMC4571307/ /pubmed/25947134 http://dx.doi.org/10.1091/mbc.E14-05-1002 Text en © 2015 Ollion et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Ollion, Jean Loll, François Cochennec, Julien Boudier, Thomas Escudé, Christophe Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines |
title | Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines |
title_full | Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines |
title_fullStr | Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines |
title_full_unstemmed | Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines |
title_short | Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines |
title_sort | proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571307/ https://www.ncbi.nlm.nih.gov/pubmed/25947134 http://dx.doi.org/10.1091/mbc.E14-05-1002 |
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