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Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines

The cell nucleus is a highly organized structure and plays an important role in gene regulation. Understanding the mechanisms that sustain this organization is therefore essential for understanding genome function. Centromeric regions (CRs) of chromosomes have been known for years to adopt specific...

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Autores principales: Ollion, Jean, Loll, François, Cochennec, Julien, Boudier, Thomas, Escudé, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571307/
https://www.ncbi.nlm.nih.gov/pubmed/25947134
http://dx.doi.org/10.1091/mbc.E14-05-1002
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author Ollion, Jean
Loll, François
Cochennec, Julien
Boudier, Thomas
Escudé, Christophe
author_facet Ollion, Jean
Loll, François
Cochennec, Julien
Boudier, Thomas
Escudé, Christophe
author_sort Ollion, Jean
collection PubMed
description The cell nucleus is a highly organized structure and plays an important role in gene regulation. Understanding the mechanisms that sustain this organization is therefore essential for understanding genome function. Centromeric regions (CRs) of chromosomes have been known for years to adopt specific nuclear positioning patterns, but the significance of this observation is not yet completely understood. Here, using a combination of fluorescence in situ hybridization and immunochemistry on fixed human cells and high-throughput imaging, we directly and quantitatively investigated the nuclear positioning of specific human CRs. We observe differential attraction of individual CRs toward both the nuclear border and the nucleoli, the former being enhanced in nonproliferating cells and the latter being enhanced in proliferating cells. Similar positioning patterns are observed in two different lymphoblastoid cell lines. Moreover, the positioning of CRs differs from that of noncentromeric regions, and CRs display specific orientations within chromosome territories. These results suggest the existence of not-yet-characterized mechanisms that drive the nuclear positioning of CRs and therefore pave the way toward a better understanding of how CRs affect nuclear organization.
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spelling pubmed-45713072015-09-29 Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines Ollion, Jean Loll, François Cochennec, Julien Boudier, Thomas Escudé, Christophe Mol Biol Cell Articles The cell nucleus is a highly organized structure and plays an important role in gene regulation. Understanding the mechanisms that sustain this organization is therefore essential for understanding genome function. Centromeric regions (CRs) of chromosomes have been known for years to adopt specific nuclear positioning patterns, but the significance of this observation is not yet completely understood. Here, using a combination of fluorescence in situ hybridization and immunochemistry on fixed human cells and high-throughput imaging, we directly and quantitatively investigated the nuclear positioning of specific human CRs. We observe differential attraction of individual CRs toward both the nuclear border and the nucleoli, the former being enhanced in nonproliferating cells and the latter being enhanced in proliferating cells. Similar positioning patterns are observed in two different lymphoblastoid cell lines. Moreover, the positioning of CRs differs from that of noncentromeric regions, and CRs display specific orientations within chromosome territories. These results suggest the existence of not-yet-characterized mechanisms that drive the nuclear positioning of CRs and therefore pave the way toward a better understanding of how CRs affect nuclear organization. The American Society for Cell Biology 2015-07-01 /pmc/articles/PMC4571307/ /pubmed/25947134 http://dx.doi.org/10.1091/mbc.E14-05-1002 Text en © 2015 Ollion et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Ollion, Jean
Loll, François
Cochennec, Julien
Boudier, Thomas
Escudé, Christophe
Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines
title Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines
title_full Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines
title_fullStr Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines
title_full_unstemmed Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines
title_short Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines
title_sort proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571307/
https://www.ncbi.nlm.nih.gov/pubmed/25947134
http://dx.doi.org/10.1091/mbc.E14-05-1002
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