Cargando…
A Novel Targeted Screening Tool for Hypogammaglobulinemia: Measurement of Serum Immunoglobulin (IgG, IgM, IgA) Levels from Dried Blood Spots (Ig-DBS Assay)
PURPOSE: To develop an assay to quantify serum immunoglobulin (IgG, IgM, IgA) levels using dried blood spots (DBS) obtained on collection cards to be used as a tool for targeted screening for hypogammaglobulinemia. METHODS: DBS samples, along with simultaneous serum samples, were collected from 107...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572045/ https://www.ncbi.nlm.nih.gov/pubmed/26275445 http://dx.doi.org/10.1007/s10875-015-0184-y |
_version_ | 1782390383081160704 |
---|---|
author | Yel, Leman Rabbat, Christopher J. Cunningham-Rundles, Charlotte Orange, Jordan S. Torgerson, Troy R. Verbsky, James W. Wang, Yeong Fu, Maoyong Robins, Terry S. Edwards, Marc S. Nymann-Andersen, Jesper |
author_facet | Yel, Leman Rabbat, Christopher J. Cunningham-Rundles, Charlotte Orange, Jordan S. Torgerson, Troy R. Verbsky, James W. Wang, Yeong Fu, Maoyong Robins, Terry S. Edwards, Marc S. Nymann-Andersen, Jesper |
author_sort | Yel, Leman |
collection | PubMed |
description | PURPOSE: To develop an assay to quantify serum immunoglobulin (IgG, IgM, IgA) levels using dried blood spots (DBS) obtained on collection cards to be used as a tool for targeted screening for hypogammaglobulinemia. METHODS: DBS samples, along with simultaneous serum samples, were collected from 107 healthy individuals (11 months to 57 years of age). After eluting proteins from DBS, IgG, IgM, and IgA were quantified by an enzyme-linked immunosorbent assay (ELISA). The Ig-DBS assay was validated through calibration curve performance, intra- and inter-assay precision, accuracy, specificity, selectivity, and linearity. The ELISA measurements were compared with serum Ig levels obtained using a standard nephelometry assay on serum samples collected simultaneously with the DBS samples and the results of the two assays were correlated. The stability of IgG, IgM, and IgA in the DBS was tested at room temperature, 36° to 38 °C, 2 to 8 °C, and −25 to −40 °C, from 4 to 14 days. RESULTS: The Ig-DBS assay demonstrated precision, accuracy, specificity, selectivity, and linearity. Using the identified correlation coefficients of 0.834 for IgG, 0.789 for IgM, and 0.918 for IgA, the standard nephelometry-based normal reference ranges for all 3 serum Ig isotypes could be used with the Ig-DBS assay in individuals ≥16 years of age. The DBS samples were stable for 14 days at room temperature in a closed polyethylene bag. CONCLUSIONS: The Ig-DBS assay is both sensitive and accurate for quantification of serum immunoglobulins. Samples are sufficiently stable at ambient temperature to allow for convenient shipping and analysis at a centralized laboratory. This assay therefore presents a new option for screening patients ≥16 years of age for hypogammaglobulinemia in any setting. |
format | Online Article Text |
id | pubmed-4572045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-45720452015-09-23 A Novel Targeted Screening Tool for Hypogammaglobulinemia: Measurement of Serum Immunoglobulin (IgG, IgM, IgA) Levels from Dried Blood Spots (Ig-DBS Assay) Yel, Leman Rabbat, Christopher J. Cunningham-Rundles, Charlotte Orange, Jordan S. Torgerson, Troy R. Verbsky, James W. Wang, Yeong Fu, Maoyong Robins, Terry S. Edwards, Marc S. Nymann-Andersen, Jesper J Clin Immunol Original Research PURPOSE: To develop an assay to quantify serum immunoglobulin (IgG, IgM, IgA) levels using dried blood spots (DBS) obtained on collection cards to be used as a tool for targeted screening for hypogammaglobulinemia. METHODS: DBS samples, along with simultaneous serum samples, were collected from 107 healthy individuals (11 months to 57 years of age). After eluting proteins from DBS, IgG, IgM, and IgA were quantified by an enzyme-linked immunosorbent assay (ELISA). The Ig-DBS assay was validated through calibration curve performance, intra- and inter-assay precision, accuracy, specificity, selectivity, and linearity. The ELISA measurements were compared with serum Ig levels obtained using a standard nephelometry assay on serum samples collected simultaneously with the DBS samples and the results of the two assays were correlated. The stability of IgG, IgM, and IgA in the DBS was tested at room temperature, 36° to 38 °C, 2 to 8 °C, and −25 to −40 °C, from 4 to 14 days. RESULTS: The Ig-DBS assay demonstrated precision, accuracy, specificity, selectivity, and linearity. Using the identified correlation coefficients of 0.834 for IgG, 0.789 for IgM, and 0.918 for IgA, the standard nephelometry-based normal reference ranges for all 3 serum Ig isotypes could be used with the Ig-DBS assay in individuals ≥16 years of age. The DBS samples were stable for 14 days at room temperature in a closed polyethylene bag. CONCLUSIONS: The Ig-DBS assay is both sensitive and accurate for quantification of serum immunoglobulins. Samples are sufficiently stable at ambient temperature to allow for convenient shipping and analysis at a centralized laboratory. This assay therefore presents a new option for screening patients ≥16 years of age for hypogammaglobulinemia in any setting. Springer US 2015-08-01 2015 /pmc/articles/PMC4572045/ /pubmed/26275445 http://dx.doi.org/10.1007/s10875-015-0184-y Text en © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Research Yel, Leman Rabbat, Christopher J. Cunningham-Rundles, Charlotte Orange, Jordan S. Torgerson, Troy R. Verbsky, James W. Wang, Yeong Fu, Maoyong Robins, Terry S. Edwards, Marc S. Nymann-Andersen, Jesper A Novel Targeted Screening Tool for Hypogammaglobulinemia: Measurement of Serum Immunoglobulin (IgG, IgM, IgA) Levels from Dried Blood Spots (Ig-DBS Assay) |
title | A Novel Targeted Screening Tool for Hypogammaglobulinemia: Measurement of Serum Immunoglobulin (IgG, IgM, IgA) Levels from Dried Blood Spots (Ig-DBS Assay) |
title_full | A Novel Targeted Screening Tool for Hypogammaglobulinemia: Measurement of Serum Immunoglobulin (IgG, IgM, IgA) Levels from Dried Blood Spots (Ig-DBS Assay) |
title_fullStr | A Novel Targeted Screening Tool for Hypogammaglobulinemia: Measurement of Serum Immunoglobulin (IgG, IgM, IgA) Levels from Dried Blood Spots (Ig-DBS Assay) |
title_full_unstemmed | A Novel Targeted Screening Tool for Hypogammaglobulinemia: Measurement of Serum Immunoglobulin (IgG, IgM, IgA) Levels from Dried Blood Spots (Ig-DBS Assay) |
title_short | A Novel Targeted Screening Tool for Hypogammaglobulinemia: Measurement of Serum Immunoglobulin (IgG, IgM, IgA) Levels from Dried Blood Spots (Ig-DBS Assay) |
title_sort | novel targeted screening tool for hypogammaglobulinemia: measurement of serum immunoglobulin (igg, igm, iga) levels from dried blood spots (ig-dbs assay) |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572045/ https://www.ncbi.nlm.nih.gov/pubmed/26275445 http://dx.doi.org/10.1007/s10875-015-0184-y |
work_keys_str_mv | AT yelleman anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT rabbatchristopherj anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT cunninghamrundlescharlotte anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT orangejordans anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT torgersontroyr anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT verbskyjamesw anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT wangyeong anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT fumaoyong anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT robinsterrys anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT edwardsmarcs anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT nymannandersenjesper anoveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT yelleman noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT rabbatchristopherj noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT cunninghamrundlescharlotte noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT orangejordans noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT torgersontroyr noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT verbskyjamesw noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT wangyeong noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT fumaoyong noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT robinsterrys noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT edwardsmarcs noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay AT nymannandersenjesper noveltargetedscreeningtoolforhypogammaglobulinemiameasurementofserumimmunoglobuliniggigmigalevelsfromdriedbloodspotsigdbsassay |