Systematic Evaluation of Bioorthogonal Reactions in Live Cells with Clickable HaloTag Ligands: Implications for Intracellular Imaging

[Image: see text] Bioorthogonal reactions, including the strain-promoted azide–alkyne cycloaddition (SPAAC) and inverse electron demand Diels–Alder (iEDDA) reactions, have become increasingly popular for live-cell imaging applications. However, the stability and reactivity of reagents has never been...

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Detalles Bibliográficos
Autores principales: Murrey, Heather E., Judkins, Joshua C., am Ende, Christopher W., Ballard, T. Eric, Fang, Yinzhi, Riccardi, Keith, Di, Li, Guilmette, Edward R., Schwartz, Joel W., Fox, Joseph M., Johnson, Douglas S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572613/
https://www.ncbi.nlm.nih.gov/pubmed/26270632
http://dx.doi.org/10.1021/jacs.5b06847

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