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High-performance probes for light and electron microscopy

We describe an engineered family of highly antigenic molecules based on GFP-like fluorescent proteins. These molecules contain numerous copies of peptide epitopes and simultaneously bind IgG antibodies at each location. These “spaghetti monster” fluorescent proteins (smFPs) distribute well in neuron...

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Detalles Bibliográficos
Autores principales: Viswanathan, Sarada, Williams, Megan E., Bloss, Erik B., Stasevich, Timothy J., Speer, Colenso M., Nern, Aljoscha, Pfeiffer, Barret D., Hooks, Bryan M., Li, Wei-Ping, English, Brian P., Tian, Teresa, Henry, Gilbert L., Macklin, John J., Patel, Ronak, Gerfen, Charles R., Zhuang, Xiaowei, Wang, Yalin, Rubin, Gerald M., Looger, Loren L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573404/
https://www.ncbi.nlm.nih.gov/pubmed/25915120
http://dx.doi.org/10.1038/nmeth.3365