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Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell

BACKGROUND AND PURPOSE: Dysregulated miRNAs play an important role in many malignant tumors. However, elucidating the roles of miRNAs in cancer biology, especially in epithelial cancers, remains an ongoing process. In this study, we identified the differentially expressed miR-645 in the progressing...

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Autores principales: Sun, Qiang, Chen, Shuai, Zhao, Xue, Yan, Ming, Fang, Zheng, Wang, Haibin, Zhao, Junfang, Sun, Minglei, Han, Xinguang, Chen, Wantao, Li, Xinming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573489/
https://www.ncbi.nlm.nih.gov/pubmed/26388702
http://dx.doi.org/10.1186/s12935-015-0238-5
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author Sun, Qiang
Chen, Shuai
Zhao, Xue
Yan, Ming
Fang, Zheng
Wang, Haibin
Zhao, Junfang
Sun, Minglei
Han, Xinguang
Chen, Wantao
Li, Xinming
author_facet Sun, Qiang
Chen, Shuai
Zhao, Xue
Yan, Ming
Fang, Zheng
Wang, Haibin
Zhao, Junfang
Sun, Minglei
Han, Xinguang
Chen, Wantao
Li, Xinming
author_sort Sun, Qiang
collection PubMed
description BACKGROUND AND PURPOSE: Dysregulated miRNAs play an important role in many malignant tumors. However, elucidating the roles of miRNAs in cancer biology, especially in epithelial cancers, remains an ongoing process. In this study, we identified the differentially expressed miR-645 in the progressing of head and neck squamous cell carcinoma (HNSCC) and investigated its biological function. METHODS: The association between clinicopathological parameters and the expression levels of the candidated miRNAs were analyzed by using the Kaplan–Meier survival analysis. The cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells. RESULTS: In the 34 HNSCC tissues with lymph node metastasis, the expression level of miR-645 was 0.54 ± 0.12, and the expression level was 0.22 ± 0.05 in the 28 tissues with non lymph node metastasis (p = 0.017). In patients with HNSCC, higher level of miR-645 expression significantly correlates with worse overall survival (p = 0.04). Ectopic expression of miR-645 promoted cell invasion and migration. CONCLUSIONS: miR-645 play a key role in cell invasion and metastasis and their expression correlates with overall survival in the patients with HNSCC.
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spelling pubmed-45734892015-09-19 Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell Sun, Qiang Chen, Shuai Zhao, Xue Yan, Ming Fang, Zheng Wang, Haibin Zhao, Junfang Sun, Minglei Han, Xinguang Chen, Wantao Li, Xinming Cancer Cell Int Primary Research BACKGROUND AND PURPOSE: Dysregulated miRNAs play an important role in many malignant tumors. However, elucidating the roles of miRNAs in cancer biology, especially in epithelial cancers, remains an ongoing process. In this study, we identified the differentially expressed miR-645 in the progressing of head and neck squamous cell carcinoma (HNSCC) and investigated its biological function. METHODS: The association between clinicopathological parameters and the expression levels of the candidated miRNAs were analyzed by using the Kaplan–Meier survival analysis. The cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells. RESULTS: In the 34 HNSCC tissues with lymph node metastasis, the expression level of miR-645 was 0.54 ± 0.12, and the expression level was 0.22 ± 0.05 in the 28 tissues with non lymph node metastasis (p = 0.017). In patients with HNSCC, higher level of miR-645 expression significantly correlates with worse overall survival (p = 0.04). Ectopic expression of miR-645 promoted cell invasion and migration. CONCLUSIONS: miR-645 play a key role in cell invasion and metastasis and their expression correlates with overall survival in the patients with HNSCC. BioMed Central 2015-09-17 /pmc/articles/PMC4573489/ /pubmed/26388702 http://dx.doi.org/10.1186/s12935-015-0238-5 Text en © Sun et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Primary Research
Sun, Qiang
Chen, Shuai
Zhao, Xue
Yan, Ming
Fang, Zheng
Wang, Haibin
Zhao, Junfang
Sun, Minglei
Han, Xinguang
Chen, Wantao
Li, Xinming
Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell
title Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell
title_full Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell
title_fullStr Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell
title_full_unstemmed Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell
title_short Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell
title_sort dysregulated mir-645 affects the proliferation and invasion of head and neck cancer cell
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573489/
https://www.ncbi.nlm.nih.gov/pubmed/26388702
http://dx.doi.org/10.1186/s12935-015-0238-5
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