Cargando…

A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats

OBJECTIVES: This study was performed to analyze the single-dose oral toxicity of the super key (processed sulfur). METHODS: All experiments were conducted at Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investig...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jinhee, Lee, Jongcheol, Kim, Sungchul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KOREAN PHARMACOPUNCTURE INSTITUTE 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573809/
https://www.ncbi.nlm.nih.gov/pubmed/26392913
http://dx.doi.org/10.3831/KPI.2015.18.026
_version_ 1782390522416988160
author Kim, Jinhee
Lee, Jongcheol
Kim, Sungchul
author_facet Kim, Jinhee
Lee, Jongcheol
Kim, Sungchul
author_sort Kim, Jinhee
collection PubMed
description OBJECTIVES: This study was performed to analyze the single-dose oral toxicity of the super key (processed sulfur). METHODS: All experiments were conducted at Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate the oral toxicity of super key We administered it orally to Sprague-Dawley (SD) rats. The SD rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of super key 500 mg/kg, 1,000 mg/kg and 2,000 mg/kg were administered to the experimental groups, and a dose of normal saline solution, 10 mL/kg, was administered to the control group. We examined the survival rates, weights, clinical signs, gross findings and necropsy findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. (Approval number: A01-14018). RESULTS: No deaths or abnormalities occurred in any of the four groups. Although slight decreases in the weights of some female rats were noted, no significant changes in weights or differences in the gross findings between the control group and the experimental groups were observed. To check for abnormalities in organs, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs. CONCLUSION: The results of this research showed that administration of 500 ─ 2,000 mg/kg of super key did not cause any changes in the weights or in the results of necropsy examinations. Neither did it result in any mortalities. The above findings suggest that treatment with super key is relatively safe. Further studies on this subject are needed to yield more concrete evidence.
format Online
Article
Text
id pubmed-4573809
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher KOREAN PHARMACOPUNCTURE INSTITUTE
record_format MEDLINE/PubMed
spelling pubmed-45738092015-09-18 A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats Kim, Jinhee Lee, Jongcheol Kim, Sungchul J Pharmacopuncture Original Article OBJECTIVES: This study was performed to analyze the single-dose oral toxicity of the super key (processed sulfur). METHODS: All experiments were conducted at Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate the oral toxicity of super key We administered it orally to Sprague-Dawley (SD) rats. The SD rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of super key 500 mg/kg, 1,000 mg/kg and 2,000 mg/kg were administered to the experimental groups, and a dose of normal saline solution, 10 mL/kg, was administered to the control group. We examined the survival rates, weights, clinical signs, gross findings and necropsy findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. (Approval number: A01-14018). RESULTS: No deaths or abnormalities occurred in any of the four groups. Although slight decreases in the weights of some female rats were noted, no significant changes in weights or differences in the gross findings between the control group and the experimental groups were observed. To check for abnormalities in organs, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs. CONCLUSION: The results of this research showed that administration of 500 ─ 2,000 mg/kg of super key did not cause any changes in the weights or in the results of necropsy examinations. Neither did it result in any mortalities. The above findings suggest that treatment with super key is relatively safe. Further studies on this subject are needed to yield more concrete evidence. KOREAN PHARMACOPUNCTURE INSTITUTE 2015-09 /pmc/articles/PMC4573809/ /pubmed/26392913 http://dx.doi.org/10.3831/KPI.2015.18.026 Text en Copyright ©2015, KOREAN PHARMACOPUNCTURE INSTITUTE http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Jinhee
Lee, Jongcheol
Kim, Sungchul
A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats
title A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats
title_full A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats
title_fullStr A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats
title_full_unstemmed A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats
title_short A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats
title_sort study on the single-dose oral toxicity of super key in sprague-dawley rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573809/
https://www.ncbi.nlm.nih.gov/pubmed/26392913
http://dx.doi.org/10.3831/KPI.2015.18.026
work_keys_str_mv AT kimjinhee astudyonthesingledoseoraltoxicityofsuperkeyinspraguedawleyrats
AT leejongcheol astudyonthesingledoseoraltoxicityofsuperkeyinspraguedawleyrats
AT kimsungchul astudyonthesingledoseoraltoxicityofsuperkeyinspraguedawleyrats
AT kimjinhee studyonthesingledoseoraltoxicityofsuperkeyinspraguedawleyrats
AT leejongcheol studyonthesingledoseoraltoxicityofsuperkeyinspraguedawleyrats
AT kimsungchul studyonthesingledoseoraltoxicityofsuperkeyinspraguedawleyrats