Cargando…

The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue

BACKGROUND: I t is established that adipose-derived stem cells (ADSCs) produce and secrete cytokines/growth factors that antagonize mucosal injury. However, the exact molecular basis underlying the treatment effects exerted by ADSCs is ill understood, and whether ADSCs cooperate with adipose tissue...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Xiao, Li, Liang, Wang, Cheng, Liu, Yang, Chen, Chong, Yan, Junling, Ding, Hong, Tang, Su-yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574024/
https://www.ncbi.nlm.nih.gov/pubmed/26388989
http://dx.doi.org/10.1186/s13578-015-0045-7
_version_ 1782390551900848128
author Xu, Xiao
Li, Liang
Wang, Cheng
Liu, Yang
Chen, Chong
Yan, Junling
Ding, Hong
Tang, Su-yang
author_facet Xu, Xiao
Li, Liang
Wang, Cheng
Liu, Yang
Chen, Chong
Yan, Junling
Ding, Hong
Tang, Su-yang
author_sort Xu, Xiao
collection PubMed
description BACKGROUND: I t is established that adipose-derived stem cells (ADSCs) produce and secrete cytokines/growth factors that antagonize mucosal injury. However, the exact molecular basis underlying the treatment effects exerted by ADSCs is ill understood, and whether ADSCs cooperate with adipose tissue particles to improve mucosal function in patients with empty nose syndrome (ENS) has not been explored. We investigated the impact of ADSCs on nasal mucosa, the associated mechanisms, and their use in the treatment of patients with ENS. RESULTS: The nasal endoscope and mucociliary clearance assessments were significantly improved (P < 0.05) in patients with (n = 28) and without (n = 2) a rudimentary turbinate that received ADSCs combined with fat granules transplantation. Patients experienced a significant improvement in nasal obstruction and nasal mucociliary clearance after nasal turbinate angioplasty (P < 0.05). H&E staining, Masson’s staining, and AB-PAS staining confirmed that inflammation was significantly reduced, collagenous fibers became aligned, fewer deposits were observed, and the mucosal proteins generated from caliciform cells increased following treatment. After a 14-day incubation period, ADSCs developed a polygonal cobblestone shape characteristic of human epithelial cells. Furthermore, immunohistochemical analysis revealed the presence of epithelial markers such as cytokeratin-7, and cytokeratin-19. Western blot analysis showed the presence of specific epithelial cell markers including cytokeratin-7, cytokeratin-14 and cytokeratin-19 in these epithelial like cells (ELC); these markers had low expression levels of ADSCs. CONCLUSIONS: The reconstruction of mucosal function by nasal turbinate angioplasty combined with ADSCs and autologous adipose tissue particle transplantation significantly improved the symptoms of patients with ENS. This is a new procedure that will improve mucosal restoration treatment options in patients with ENS. Furthermore, we undertook preliminary explorations of the underlying mechanisms involved, and found that transplantation of ADSCs could induce epithelial cells to improve mucosa function in patients with ENS in the micro-environment of injection areas.
format Online
Article
Text
id pubmed-4574024
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-45740242015-09-19 The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue Xu, Xiao Li, Liang Wang, Cheng Liu, Yang Chen, Chong Yan, Junling Ding, Hong Tang, Su-yang Cell Biosci Research BACKGROUND: I t is established that adipose-derived stem cells (ADSCs) produce and secrete cytokines/growth factors that antagonize mucosal injury. However, the exact molecular basis underlying the treatment effects exerted by ADSCs is ill understood, and whether ADSCs cooperate with adipose tissue particles to improve mucosal function in patients with empty nose syndrome (ENS) has not been explored. We investigated the impact of ADSCs on nasal mucosa, the associated mechanisms, and their use in the treatment of patients with ENS. RESULTS: The nasal endoscope and mucociliary clearance assessments were significantly improved (P < 0.05) in patients with (n = 28) and without (n = 2) a rudimentary turbinate that received ADSCs combined with fat granules transplantation. Patients experienced a significant improvement in nasal obstruction and nasal mucociliary clearance after nasal turbinate angioplasty (P < 0.05). H&E staining, Masson’s staining, and AB-PAS staining confirmed that inflammation was significantly reduced, collagenous fibers became aligned, fewer deposits were observed, and the mucosal proteins generated from caliciform cells increased following treatment. After a 14-day incubation period, ADSCs developed a polygonal cobblestone shape characteristic of human epithelial cells. Furthermore, immunohistochemical analysis revealed the presence of epithelial markers such as cytokeratin-7, and cytokeratin-19. Western blot analysis showed the presence of specific epithelial cell markers including cytokeratin-7, cytokeratin-14 and cytokeratin-19 in these epithelial like cells (ELC); these markers had low expression levels of ADSCs. CONCLUSIONS: The reconstruction of mucosal function by nasal turbinate angioplasty combined with ADSCs and autologous adipose tissue particle transplantation significantly improved the symptoms of patients with ENS. This is a new procedure that will improve mucosal restoration treatment options in patients with ENS. Furthermore, we undertook preliminary explorations of the underlying mechanisms involved, and found that transplantation of ADSCs could induce epithelial cells to improve mucosa function in patients with ENS in the micro-environment of injection areas. BioMed Central 2015-09-17 /pmc/articles/PMC4574024/ /pubmed/26388989 http://dx.doi.org/10.1186/s13578-015-0045-7 Text en © Xu et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xu, Xiao
Li, Liang
Wang, Cheng
Liu, Yang
Chen, Chong
Yan, Junling
Ding, Hong
Tang, Su-yang
The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue
title The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue
title_full The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue
title_fullStr The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue
title_full_unstemmed The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue
title_short The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue
title_sort expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574024/
https://www.ncbi.nlm.nih.gov/pubmed/26388989
http://dx.doi.org/10.1186/s13578-015-0045-7
work_keys_str_mv AT xuxiao theexpansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT liliang theexpansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT wangcheng theexpansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT liuyang theexpansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT chenchong theexpansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT yanjunling theexpansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT dinghong theexpansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT tangsuyang theexpansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT xuxiao expansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT liliang expansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT wangcheng expansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT liuyang expansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT chenchong expansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT yanjunling expansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT dinghong expansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue
AT tangsuyang expansionofautologousadiposederivedstemcellsinvitroforthefunctionalreconstructionofnasalmucosaltissue